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Antibiotic Susceptibility And Phylogenetic Analyses For The Origins And Serotypes Of Listeria Monocytogenes Strains Isolated From Ice Cream And Cream Cakes, Orkun Babacan

Turkish Journal of Veterinary & Animal Sciences

Listeria monocytogenes is a zoonotic bacterium which also infects humans. The aim of this study was to isolate this organism from cream cakes and ice cream and obtain 16S rRNA and hlyA gene sequences from isolates in order to perform phylogenetic analyses. Serotypes and antibiotic susceptibility were also determined. The cream cake and ice cream samples were examined for L. monocytogenes according to ISO 11290-1 and using the mini Vidas LMO 2 kit procedure. Antibiotic susceptibilities were investigated using the disc diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the Clinical and Laboratory Standards Institute …

Detection Of The Contamination Sources Of Listeria Monocytogenes In Pickled White Cheese Production Process Line And Genotyping With The Pulsed-Field Gel Electrophoresis Method, Ni̇hat Telli̇, Ahmet Güner, Ferda Soyer Dönmez, Özgün Öykü Özdemi̇r

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the contamination sources, serotyping profiles, and antibiotic resistance patterns of Listeria monocytogenes isolated during the production of pickled white cheese. The genetic-relatedness of the isolates to EGD SLCC (5835) (1/2a, lineage II) and ATCC (13932) (4b, lineage I) reference strains was also determined with pulsed-field gel electrophoresis (PFGE) as a result of digestions with AscI and ApaI enzymes. Samples were collected from 16 different points in the production process of 4 different plants at 3 different times. Among the 192 samples examined, 17 (8.85%) were determined to be contaminated with Listeria spp. Three isolates …

Serotyping And Antibiotic Susceptibility Of Listeria Monocytogenes Isolatedfrom Ready-To-Eat Foods In Samsun, Turkey, Göknur Terzi̇, Ali̇ Gücükoğlu, Özgür Çadirci, Tolga Uyanik, Mustafa Ali̇şarli

Turkish Journal of Veterinary & Animal Sciences

The objective of the present study was to assess the presence of Listeria monocytogenes and to investigate its serotyping and antibiotic resistance in ready-to-eat (RTE) foods. A total of 100 RTE foods, including 25 stuffed mussels, 25 ezme samples (a Turkish-style tomato dip/condiment), 25 fried spiced livers, and 25 mayonnaise-based salads were obtained from retail shops and supermarkets in Samsun, Turkey. Samples were analyzed using the standard procedure EN ISO 11290-1 and isolates of L. monocytogenes were confirmed for the presence of the hemolysin gene (hylA) by polymerase chain reaction. L. monocytogenes was identified in 1 of 25 (4%) mayonnaise-based …

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Michael Greger, MD, FACLM

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.

Listeria Monocytogenes In Mussels (Mytilus Galloprovincialis) Harvested From North Aegean Coastal Area, Nikolaos Soultos, Eleni Iossifidou, Amin Ambrahim, Evdoxios Psomas, Ioannis Tzavaras, Demetrios Koutsopoulos, Thomai Lazou

Turkish Journal of Veterinary & Animal Sciences

One hundred and two samples of mussels (Mytilus galloprovincialis), harvested from approved shellfish coastal water in northern Greece, were screened for the presence and antimicrobial resistance of Listeria monocytogenes. Listeria spp. were isolated according to International Organization for Standardization method 11290-1: 1996/FDAM 1: 2004(E) and identified using a multiplex polymerase chain reaction (PCR) system. The serovar identity of L. monocytogenes isolates was also determined with a multiplex PCR assay. The antimicrobial profile of the isolates was determined by the disk diffusion method. Listeria spp. were present in 8 of 102 samples tested (8%) and only 1 (1%) yielded L. monocytogenes. …

Prevalence Of Listeria Monocytogenes In Chicken Production Chain In Thailand, Sasitorn Kanarat, Wongkwan Jitnupong, Jananya Sukhapesna

The Thai Journal of Veterinary Medicine

This study was conducted from 2004 to 2009 to investigate the prevalence of Listeria monocytogenes in thechicken production chain in Thailand from primary production stages to processing plants. A total of 14,670 sampleswere taken from 43 breeder farms, 32 hatcheries, 1331 broiler farms, 22 slaughterhouses and 22 ready-to-eat (RTE)chicken products from processing plants. Various types of samples were collected: soiled litter, water, and chickenfeed from breeder and broiler farms; swabs and mecomium from hatcheries; cloacal swabs and fresh frozen chickenmeat from slaughterhouses; and RTE chicken products from processing plants. This study showed that there was noL. monocytogenes contamination in the …

Prevalence And Antibiotic Resistance Of Listeria Spp. Isolated From Ready-To-Eat Foods In Ankara, Ufuk Tansel Şi̇reli̇, Ali̇ Gücükoğlu

Turkish Journal of Veterinary & Animal Sciences

In this study the presence of Listeria spp. is tested in 100 ready-to-eat food samples purchased from different stores and traditional food shops in the province of Ankara. The tested materials were 20 each of the following: mayonnaise based salad, kadınbudu köfte (fried meatball), fried liver, rice stuffed mussel, and green salad. Microbiological analyzes showed that 13 of 100 salad samples (13%) were contaminated with Listeria spp. while 10 of 100 salad samples (10%) were contaminated with Listeria monocytogenes. Among these, 4 of 20 mayonnaise based salad samples (20%), 1 of 20 fried liver (5%), 1 of 20 rice stuffed …

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Transgenesis Collection

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.

The Seroprevalence Of Listeria Monocytogenes In Sport Horses Bred In Ankara Province, H. Zeynep Güçlü, K. Zafer Karaer, Cahi̇t Babür, Selçuk Kiliç

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the seroprevalence of Listeria monocytogenes in horses bred for sport in the province of Ankara. Sera collected from 100 clinically healthy horses were tested for the presence of L. monocytogenes antibodies using the Osebold absorption test. Agglutination titers >= 1:100 were accepted as positive results and a seropositivity rate was determined. Of the 100 serum samples, 62 were seropositive for L. monocytogenes antibodies detected at dilutions of 1:100 (n = 29, 46.7%), 1:200 (n = 31, 50%), and 1:400 (n = 2, 3.2%).

Comparison Of Polymerase Chain Reaction And Conventional Methods For The Diagnosis Of Listeria Monocytogenes In Stuffed Mussels, Ergün Ö. Göksoy, Şükrü Kirkan, Osman Kaya

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.

Detection Of Listeria Monocytogenes By Using Pcr In Helix Pomatia, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya

Turkish Journal of Veterinary & Animal Sciences

The detection of Listeria monocytogenes in Helix pomatia, both raw and cooked is presented here. Polymerase chain reaction (PCR) is a rapid procedure with both sensitivity and specificity for quick detection and identification of Listeria monocytogenes from raw and cooked Helix pomatia. A total of 30 bags (10 g each) of H. pomatia samples were investigated for the Listeria monocytogenes inlB gene with the PCR method. PCR amplification products demonstrated that 18 of the 30 samples showed positive reactions to Listeria monocytogenes in the PCR. All PCR positive samples showed specific amplification of the 343 bp fragment for Listeria monocytogenes.

Thermal Resistance Of Listeria Monocytogenes In İnegöl Meatballs, G. Ece Soyutemi̇z, Fi̇gen Çeti̇nkaya

Turkish Journal of Veterinary & Animal Sciences

This study was performed to determine the thermal resistance of L. monocytogenes at different heat treatments in İnegöl meatballs. İnegöl meatballs were divided into three groups. In group I, meatballs were inoculated with 8.0x10^2, 1.9x10^3, 6.0x10^3 and 2.4x10^4 cfu/g L. monocytogenes and then they were cooked at an internal temperature of 85 °C for 4 min. Group II meatballs were inoculated with 1.0x10^2, 2.0x10^2, 8.0x10^2, 5.6x10^5 and 2.0x10^6 cfu/g L. monocytogenes and cooked at an internal temperature of 80 °C for 4 min. In group III, the meatballs were inoculated with 5.6x10^5 and 2.0x10^6 cfu/g L. monocytogenes and cooked at …

Isolation Of Listeria Monocytogenes From Fish Intestines And Rapd Analysis, Hasan Basri̇ Ertaş, Engi̇n Şeker

Turkish Journal of Veterinary & Animal Sciences

One hundred and fifty fish caught from Keban Dam Lake were examined for the presence of Listeria monocytogenes. A random amplified polymorphic DNA (RAPD) assay using a random primer was performed to detect genetic variations among L. monocytogenes isolates. The selective enrichment procedure followed by selective isolation was used for listeria isolation from fish intestines. Ten (6.6%) L. monocytogenes were isolated and identified from fish intestines. Two different band profiles were obtained by the RAPD analysis of the isolates. The results indicated that the isolates might have originated from different sources, and fish contaminated with L. monocytogenes may cause serious …

Effect Of Lactobacillus Sakei Lb706 On Behavior Of Listeria Monocytogenes In Vacuum-Packed Rainbow Trout Fillets, Şükri̇ye Aras Hi̇sar, Güzi̇n Kaban, Olcay Hi̇sar, Telat Yanik, Mükerrem Kaya

Turkish Journal of Veterinary & Animal Sciences

The effect of a bacteriocin-producing strain, Lactobacillus sakei Lb706, on the behavior of Listeria monocytogenes in rainbow trout fillets stored under vacuum at 4 ºC for 14 days and at 10 ºC for 5 days was investigated. At 4 ºC, growth of L. monocytogenes was significantly inhibited (P < 0.05) by L. sakei Lb706 during 10 days of storage while bacteriocin-negative Lb706-B did not affect the growth of L. monocytogenes during 14 days of storage. In the presence of the sakacin A-producing strain of L. sakei (Lb706), the growth of L. monocytogenes was significantly inhibited (P < 0.05) in the first 3 days of storage at 10 ºC, after which its count increased to 10^7 CFU/g. The control group and the group containing L. sakei Lb706-B showed similar growth patterns of L. monocytogenes. Significant increases (P < 0.05) were observed in all groups in terms of psychrotrophic bacteria counts. No significant (P > 0.05) changes in pH values during storage at 4 ºC and 10 ºC were determined. Consequently, the results of the present study showed that L. sakei Lb706 can be used as a protective culture in freshwater reared rainbow trout, especially for preventing possible contaminations of Gram-positive food-borne pathogenic bacteria such as L. …

Faecal Carriage Of Listeria Monocytogenes In Stray Dogs In Bursa Province, Turkey, A. Levent Kocabiyik, Cengi̇z Çeti̇n, Cüneyt Özakin

Turkish Journal of Veterinary & Animal Sciences

The faecal carriage rate of Listeria monocytogenes in stray dogs in Bursa province, Turkey, was determined from 82 faecal samples by bacteriological methods. L. monocytogenes was isolated from only one (1.22%) of the samples examined. The isolate was serotyped as 1/2b. It was concluded that stray dogs could be a reservoir of the organism as well as a source of human listeriosis in Turkey. This is the first report on the isolation of L. monocytogenes from a dog in this country.

Listeria Monocytogenes, Yersinia Enterocolitica And Salmonella Enteritidis In Quail Eggs, Özlem Turgay

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the presence of Listeria monocytogenes, Yersinia enterocolitica and Salmonella enteritidis in 123 liquid whole quail eggs. The method suggested by USDA-FSIS was used for the isolation and identification of L. monocytogenes. S. enteritidis was identified and sero-grouped by co-agglutination test and slide agglutination test. Y. enterocolitica was isolated in Trypticase-Soy Broth, with bile-oxalate-sorbose medium for enrichment. Both enrichment cultures were plated on Yersinia Selective Agar Base containing cefsulodin, irgasan and novobiocin (CIN Agar). No Yersinia was detected among the natural flora of the quail eggs. Seven (5.69%) of 123 whole quail eggs …

A Study On Survival Of Listeria Monocytogenes During Manufacture And Ripening Of Kashar Cheese, Fi̇gen Çeti̇nkaya, G. Ece Soyutemi̇z

Turkish Journal of Veterinary & Animal Sciences

This study was performed to determine the survival of Listeria monocytogenes during the manufacture and ripening of Kashar, a popular cheese in Turkey. Raw milk was inoculated at 4 different levels with L. monocytogenes serotype 4b (ca. 3, 4, 5, and 6 log cfu ml^{-1} for group 1, 2, 3 and 4 cheeses, respectively) and made into Kashar cheese using the traditional technique. Following dry salting for 10 days at 18 ± 2 ºC, the Kashar cheese was ripened for 20 days at the same temperature and then stored at 5 ± 1 ºC for 3 months. Samples of milk, …

The Effects Of Starter Cultures And Different Nitrite Doses On The Growth Of Listeria Monocytogenes In Sucuk Production, Mükerrem Kaya, Hüsnü Yusuf Gökalp

Turkish Journal of Veterinary & Animal Sciences

The effects of different nitrite doses (50, 100, 150 and 200 ppm NaNO_2) and starter culture (Lactobacillus plantarum + Staphylococcus carnosus, a commercial preparation) on the growth of Listeria monocytogenes in sucuk (Turkish dry fermented sausage) were investigated. The initial inoculation of the L. monocytogenes was 10^4 cfu/g for each sucuk dough mix. At the beginning of the ripening period in sucuk samples containing different amounts of NaNO_2 and without starter culture, L. monocytogenes growth was observed. On the 3^{rd} day of ripening the L. monocytogenes count was over 10^7 cfu/g. No growth was detected in the sucuks containing starter …

Investigation On The Viability Span Of Listeria Monocytogenes During The Ripening Of Brined Şavak Cheese, Bahri̇ Patir, Aba Müslüm Güven

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the viability span of listeria monocytogenes during the ripening of brined şavak white cheese which is produced by and has a significant role in the nutrition of people living in Elazığ, Tunceli, Bingöl and Erzincan. Ewes milk was inoculated with various strains (4b, 1/2c and 1/2a) of listeria monocytogenes at the rate of 1.00x10^3 - 1.35x10^6 cells/ml and then şavak cheese was produced from the milk. The cheese samples were analysed after 0, 15, 30, 60, 90 and 120 days of ripening for the numbers of listeria monocytogenes with the direct plating …

Incidence And Serotype Distribution Of Listeria Monocytogenes In Frozen Broiler Carcasses, İrfan Erol, Ufuk Tansel Şi̇reli̇

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the presence of Listeria spp. and the serotype distribution of L. monocytogenes in fifty frozen broiler carcass samples obtained from different producers. USDA/FSIS suggested method was used for the isolation and identification of Listeria spp. Overall, 5 different Listeria species could be isolated from 94 % of frozen broiler carcasses. L. innocua was the most prevalent species with 90 % (45/50) followed by L. monocytogenes with 30 % (15/50), L. welshimeri with % 8 (4/50), L. grayi with 2 % (1/50) and L. murrayi with % 2 (1/50) respectively. According to the analysis there …

Effect Of Bacteriocinogenic Starter Cultures On The Growth Of Listeria Monocytogenes In Turkish Fermented Sausage (Sucuk), İrfan Erol, T. Haluk Çeli̇k, Ufuk Tansel Şi̇reli̇, Haydar Özdemi̇r

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was the inhibition of L. monocytogenes (SLCC 9488) serotype 4b, indicated great importance in foodborne listeriosis, by using bacteriocin producer starter cultures as Pediococcus acidilactici PAC 1.0 and Lactobacillus sake Lb 706 in fermented Turkish sausage (sucuk), ripened at 20° and 25°C. For this purpose, 4 groups were constituted in each experimental sucuk producing as following. All of these groups were inoculated with L. monocytogenes at the range of 7.0 - 2.9x10^5 cfu/g, and the other groups except control group following starter cultures were added. 1.Group : Included only L. monocytogenes at the range of …

Ovine Listeriosis : A Newley Diagnosed Cause Of Infectious Abortion And Lamb Losses In Western Australia, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

LISTERIOSIS is an infectious disease occurring in animals and man and is caused by the bacterium Listeria monocytogenes.

It is worldwide in distribution and has been reported in 28 species of animals and 11 species of birds.

This disease was recently diagnosed in Western Australia for the first time when it was found to be a cause of abortion and perinatal lamb losses in sheep.