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Full-Text Articles in Medicine and Health Sciences
Transient Silencing Of A Type Iv P-Type Atpase Results In Decreased Glut4 Translo-Cation And Altered Signaling Of Mapk And Pi3k Pathways, Sarah Hurst, Nolan Hoffman, Jeffrey Elmendorf, Madhu S. Dhar
Transient Silencing Of A Type Iv P-Type Atpase Results In Decreased Glut4 Translo-Cation And Altered Signaling Of Mapk And Pi3k Pathways, Sarah Hurst, Nolan Hoffman, Jeffrey Elmendorf, Madhu S. Dhar
Madhu S Dhar
SBN: 978-14775549-6-8
Transient Silencing Of A Type Iv P-Type Atpase, Atp10c, Results In Decreased Glucose Uptake In C2c12 Myotubes, S E. Hurst, S C. Minkin, J Biggerstaff, Madhu S. Dhar
Transient Silencing Of A Type Iv P-Type Atpase, Atp10c, Results In Decreased Glucose Uptake In C2c12 Myotubes, S E. Hurst, S C. Minkin, J Biggerstaff, Madhu S. Dhar
Madhu S Dhar
Atp10c is a strong candidate gene for diet-induced obesity and type 2 diabetes. To identify molecular and cellular targets of ATP10C, Atp10c expression was altered in vitro in C2C12 skeletal muscle myotubes by transient transfection with an Atp10c-specific siRNA. Glucose uptake assays revealed that insulin stimulation caused a significant 2.54-fold decrease in 2-deoxyglucose uptake in transfected cells coupled with a significant upregulation of native mitogen-activated protein kinases (MAPKs), p38, and p44/42. Additionally, glucose transporter-1 (GLUT1) was significantly upregulated; no changes in glucose transporter-4 (GLUT4) expression were observed. The involvement of MAPKs was confirmed using the specific inhibitor SB203580, which downregulated …