Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Ovarian Function And Cigarette Smoking In The Biocycle Study, Brian W. Whitcomb, Sara D. Bodach, Sunni L. Mumford, Neil J. Perkins, Maurizio Trevisan, Jean Wactawski-Wende, Aiyi Liu, Enrique F. Schisterman
Ovarian Function And Cigarette Smoking In The Biocycle Study, Brian W. Whitcomb, Sara D. Bodach, Sunni L. Mumford, Neil J. Perkins, Maurizio Trevisan, Jean Wactawski-Wende, Aiyi Liu, Enrique F. Schisterman
Brian W. Whitcomb
Cigarette smoking has been implicated in reproductive outcomes including delayed conception, but mechanisms underlying these associations remain unclear. One potential mechanism is the effect of cigarette smoking on reproductive hormones; however, studies evaluating associations between smoking and hormone levels are complicated by variability of hormones and timing of specimen collection. We evaluated smoking among women participating in the BioCycle Study, a longitudinal study of menstrual cycle function in healthy, premenopausal, regularly menstruating women (n = 259). Fertility monitors were used to help guide timing of specimen collection. Serum levels of oestradiol, progesterone, follicle-stimulating hormone (FSH), luteinising hormone (LH) and total …
Treatment Of Batch In The Detection, Calibration, And Quantification Of Immunoassays In Large-Scale Epidemiologic Studies, Brian W. Whitcomb, Neil J. Perkins, Paul S. Albert, Enrique F. Schisterman
Treatment Of Batch In The Detection, Calibration, And Quantification Of Immunoassays In Large-Scale Epidemiologic Studies, Brian W. Whitcomb, Neil J. Perkins, Paul S. Albert, Enrique F. Schisterman
Brian W. Whitcomb
Many laboratory assays measure biomarkers via a 2-stage process. Direct measurement yields relative measures that are subsequently transformed to the unit of interest by using a calibration experiment. The calibration experiment is performed within the main experiment and uses a validation set for which true values are known and relative values are measured by assays to estimate the relation between relative and absolute values. Immunoassays, polymerase chain reaction, and chromatographic approaches are among assays performed in this manner. METHODS: For studies with multiple batches, data from more than a single calibration experiment are available. Conventionally, calibration of assays based on …