Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Control Of Electrochemical And Ferryloxy Formation Kinetics Of Cyt P450s In Polyion Films By Heme Iron Spin State And Secondary Structure, Sadagopan Krishnan, Amila Abeykoon, John B. Schenkman, James F. Rusling
Control Of Electrochemical And Ferryloxy Formation Kinetics Of Cyt P450s In Polyion Films By Heme Iron Spin State And Secondary Structure, Sadagopan Krishnan, Amila Abeykoon, John B. Schenkman, James F. Rusling
UCHC Articles - Research
Voltammetry of cytochrome P450 (cyt P450) enzymes in ultrathin films with polyions was related for the first time to electronic and secondary structure. Heterogeneous electron transfer (hET) rate constants for reduction of the cyt P450s depended on heme iron spin state, with low spin cyt P450cam giving a value 40-fold larger than high spin human cyt P450 1A2, with mixed spin human P450 cyt 2E1 at an intermediate value. Asymmetric reduction–oxidation peak separations with increasing scan rates were explained by simulations featuring faster oxidation than reduction. Results are consistent with a square scheme in which oxidized and reduced forms of …
Retention Of Polarization Signatures In Shg Microscopy Of Scattering Tissues Through Optical Clearing, Oleg Nadiarnykh, Paul J. Campagnola
Retention Of Polarization Signatures In Shg Microscopy Of Scattering Tissues Through Optical Clearing, Oleg Nadiarnykh, Paul J. Campagnola
UCHC Articles - Research
Polarization responses in Second Harmonic Generation (SHG) imaging microscopy are a valuable method to quantify aspects of tissue structure, and may be a means to differentiate normal and diseased tissues. Due to multiple scattering, the polarization data is lost in turbid tissues. Here we investigate if this information can be retained through the use of optical clearing which greatly reduces the scattering coefficient and increases the corresponding mean free path. To this end, we have measured the SHG intensity as a function of laser polarization and the SHG signal anisotropy in murine tendon and striated muscle over a depth range …