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Improved Detection By Ensemble-Decision Aliquot Ranking Of Circulating Tumor Cells With Low Numbers Of A Targeted Surface Antigen, Eleanor S. Johnson, Robbyn K. Anand, Daniel T. Chiu
Improved Detection By Ensemble-Decision Aliquot Ranking Of Circulating Tumor Cells With Low Numbers Of A Targeted Surface Antigen, Eleanor S. Johnson, Robbyn K. Anand, Daniel T. Chiu
Robbyn Anand
Circulating tumor cells (CTCs) are shed from a solid tumor into the bloodstream and can seed new metastases. CTCs hold promise for cancer diagnosis and prognosis and to increase our understanding of the metastatic process. However, their low numbers in blood and varied phenotypic characteristics make their detection and isolation difficult. One source of heterogeneity among CTCs is molecular: When they leave the primary tumor, these cells must undergo a molecular transition, which increases their mobility and chance of survival in the blood. During this molecular transition, the cells lose some of their epithelial character, which is manifested by the …
New Generation Of Ensemble-Decision Aliquot Ranking Based On Simplified Microfluidic Components For Large-Capacity Trapping Of Circulating Tumor Cells, Mengxia Zhao, Wyatt C. Nelson, Bingchuan Wei, Perry G. Schiro, Bejan M. Hakimi, Eleanor S. Johnson, Robbyn K. Anand, Grace S. Gyurkey, Lisa M. White, Samuel H. Whiting, Andrew L. Coveler, Daniel T. Chiu
New Generation Of Ensemble-Decision Aliquot Ranking Based On Simplified Microfluidic Components For Large-Capacity Trapping Of Circulating Tumor Cells, Mengxia Zhao, Wyatt C. Nelson, Bingchuan Wei, Perry G. Schiro, Bejan M. Hakimi, Eleanor S. Johnson, Robbyn K. Anand, Grace S. Gyurkey, Lisa M. White, Samuel H. Whiting, Andrew L. Coveler, Daniel T. Chiu
Robbyn Anand
Ensemble-decision aliquot ranking (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. Here, we report the next generation of eDAR, where we designed and optimized a new hydrodynamic switching scheme for the active sorting step in eDAR, which provided fast cell sorting with an improved reproducibility and stability. The microfluidic chip was also simplified by incorporating a functional area for subsequent purification using microslits fabricated by standard lithography method. Using the reported second generation of eDAR, we were able to analyze 1 mL of whole-blood samples in 12.5 min, with a 95% recovery …