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Full-Text Articles in Medicine and Health Sciences
Extraction And Quantitative Determination Of Bile Acids In Feces, Armaghan Shafaei, Joanna Rees, Claus T. Christophersen, Amanda Devine, David Broadhurst, Mary C. Boyce
Extraction And Quantitative Determination Of Bile Acids In Feces, Armaghan Shafaei, Joanna Rees, Claus T. Christophersen, Amanda Devine, David Broadhurst, Mary C. Boyce
Research outputs 2014 to 2021
© 2021 Elsevier B.V. With rapid advances in gut microbiome research, fecal bile acids are increasingly being monitored as potential biomarkers of diet related disease susceptibility. As such, rapid, robust and reliable methods for their analysis are of increasing importance. Herein is described a simple extraction method for the analysis of bile acids in feces suitable for subsequent quantification by liquid chromatography and tandem mass spectrometry. A C18 column separated the analytes with excellent peak shape and retention time repeatability maintained across 800 injections. The intra-day and inter-day precision and accuracy was greater than 80%. Recoveries ranged from 83.58 to …
Data Supporting Development And Validation Of Liquid Chromatography Tandem Mass Spectrometry Method For The Quantitative Determination Of Bile Acids In Feces, Armaghan Shafaei, Joanna Rees, Claus T. Christophersen, Amanda Devine, David Broadhurst, Mary C. Boyce
Data Supporting Development And Validation Of Liquid Chromatography Tandem Mass Spectrometry Method For The Quantitative Determination Of Bile Acids In Feces, Armaghan Shafaei, Joanna Rees, Claus T. Christophersen, Amanda Devine, David Broadhurst, Mary C. Boyce
Research outputs 2014 to 2021
Measuring bile acids in feces has an important role in disease prevention, diagnosis, treatment, and can be considered a measure of health status. Therefore, the primary aim was to develop a sensitive, robust, and high throughput liquid chromatography tandem mass spectrometry method with minimal sample preparation for quantitative determination of bile acids in human feces applicable to large cohorts. Due to the chemical diversity of bile acids, their wide concentration range in feces, and the complexity of feces itself, developing a sensitive and selective analytical method for bile acids is challenging. A simple extraction method using methanol suitable for subsequent …