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Virus-Triggered Ubiquitination Of Traf3/6 By Ciap1/2 Is Essential For Induction Of Interferon- &Beta (Ifn-&Beta) And Cellular Antiviral Response, Ai-Ping Mao, Shu Li, Bo Zhong, Ying Li, Jie Yan, Qi Li, Chengwen Teng Pharm.D.. Ph.D, Hong-Bing Shu

Faculty Publications

Viral infection causes activation of transcription factors NF-κB and IRF3, which collaborate to induce type I interferons (IFNs) and cellular antiviral response. Here we show that knockdown of the E3 ubiquitin ligases cIAP1 and cIAP2 markedly inhibited virus-triggered activation of IRF3 and NF-κB as well as IFN-β induction. Knockdown of cIAP1 and cIAP2 also inhibited cytoplasmic dsRNA-triggered cellular antiviral response. Endogenous coimmunoprecipitation experiments indicated that viral infection caused recruitment of cIAP1 and cIAP2 to TRAF3, TRAF6, and VISA. Furthermore, we demonstrated that cIAP1- and cIAP2-mediated virus-triggered ubiquitination of TRAF3 and TRAF6. These findings suggest that virus-triggered ubiquitination of TRAF3 and …