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Full-Text Articles in Medicine and Health Sciences
The Role Of Histone H4 Biotinylation In The Structure Of Nucleosomes., Nina A. Filenko, Carol Kolar, John T. West, S. Abbie Smith, Yousef I. Hassan, Gloria E.O. Borgstahl, Janos Zempleni, Yuri L. Lyubchenko
The Role Of Histone H4 Biotinylation In The Structure Of Nucleosomes., Nina A. Filenko, Carol Kolar, John T. West, S. Abbie Smith, Yousef I. Hassan, Gloria E.O. Borgstahl, Janos Zempleni, Yuri L. Lyubchenko
Journal Articles: Pharmaceutical Sciences
BACKGROUND: Post-translational modifications of histones play important roles in regulating nucleosome structure and gene transcription. It has been shown that biotinylation of histone H4 at lysine-12 in histone H4 (K12Bio-H4) is associated with repression of a number of genes. We hypothesized that biotinylation modifies the physical structure of nucleosomes, and that biotin-induced conformational changes contribute to gene silencing associated with histone biotinylation.
METHODOLOGY/PRINCIPAL FINDINGS: To test this hypothesis we used atomic force microscopy to directly analyze structures of nucleosomes formed with biotin-modified and non-modified H4. The analysis of the AFM images revealed a 13% increase in the length of DNA …