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Impact Of Fibronectin Knockout On Proliferation And Differentiation Of Human Infrapatellar Fat Pad-Derived Stem Cells, Yiming Wang, Yawen Fu, Zuoqin Yan, Xiao-Bing Zhang, Ming Pei
Impact Of Fibronectin Knockout On Proliferation And Differentiation Of Human Infrapatellar Fat Pad-Derived Stem Cells, Yiming Wang, Yawen Fu, Zuoqin Yan, Xiao-Bing Zhang, Ming Pei
Faculty & Staff Scholarship
Fibronectin plays an essential role in tissue development and regeneration. However, the effects of fibronectin knockout (FN1-KO) on stem cells’ proliferation and differentiation remain unknown. In this study, CRISPR/Cas9 generated FN1-KO in human infrapatellar fat pad-derived stem cells (IPFSCs) was evaluated for proliferation ability including cell cycle and surface markers as well as stemness gene expression and for differentiation capacity including chondrogenic and adipogenic differentiation. High passage IPFSCs were also evaluated for proliferation and differentiation capacity after expansion on decellularized ECM (dECM) deposited by FN1-KO cells. Successful FN1-KO in IPFSCs was confirmed by Sanger sequencing and Inference of CRISPR Edits …