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Full-Text Articles in Medicine and Health Sciences
Micrornas: The Short Link Between Cancer And Rt-Induced Dna Damage Response., Christopher M Wright, Tu Dan, Adam Dicker Md, Phd, Nicole L Simone
Micrornas: The Short Link Between Cancer And Rt-Induced Dna Damage Response., Christopher M Wright, Tu Dan, Adam Dicker Md, Phd, Nicole L Simone
Department of Radiation Oncology Faculty Papers
No abstract provided.
A Novel Radiation-Induced P53 Mutation Is Not Implicated In Radiation Resistance Via A Dominant-Negative Effect., Yunguang Sun, Carey Jeanne Myers, Adam Dicker Md, Phd, Bo Lu, Md
A Novel Radiation-Induced P53 Mutation Is Not Implicated In Radiation Resistance Via A Dominant-Negative Effect., Yunguang Sun, Carey Jeanne Myers, Adam Dicker Md, Phd, Bo Lu, Md
Department of Radiation Oncology Faculty Papers
Understanding the mutations that confer radiation resistance is crucial to developing mechanisms to subvert this resistance. Here we describe the creation of a radiation resistant cell line and characterization of a novel p53 mutation. Treatment with 20 Gy radiation was used to induce mutations in the H460 lung cancer cell line; radiation resistance was confirmed by clonogenic assay. Limited sequencing was performed on the resistant cells created and compared to the parent cell line, leading to the identification of a novel mutation (del) at the end of the DNA binding domain of p53. Levels of p53, phospho-p53, p21, total caspase …