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Two Alkaline Phosphatase Genes Are Expressed During Early Development In The Mouse Embryo, Ann C. Hahnel, Daniel A. Rappolee, Jose Luis Millan, Thomas Manes, Carol A. Ziomek, Nicoleta G. Theodosiou, Zena Werb, Roger A. Pedersen, Gilbert A. Schultz

Department of Obstetrics and Gynecology Research Publications

Alkaline phosphatase (AP) activity is stage specific in mouse embryos and may be associated with compaction and separation of trophectoderm from inner cell mass in preimplantation development. We previously sequenced a cDNA and two mouse AP genes that could contribute to the AP activity in embryos. Oligonucleotide primers were constructed from the three sequences and used in the reverse transcription-polymerase chain reaction technique to establish that two of the three AP isozymes are transcribed during preimplantation development. The predominant transcript (E-AP) is from a gene highly homologous to the human tissue-specific APs, but different from the mouse intestinal AP. Tissue …