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Molecular, Cellular, and Tissue Engineering
Wayne State University Associated BioMed Central Scholarship
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Detailed Characterization Of The Mouse Embryonic Stem Cell Transcriptome Reveals Novel Genes And Intergenic Splicing Associated With Pluripotency, Galih Kunarso, Kee-Yew Wong, Lawrence W. Stanton, Leonard Lipovich
Detailed Characterization Of The Mouse Embryonic Stem Cell Transcriptome Reveals Novel Genes And Intergenic Splicing Associated With Pluripotency, Galih Kunarso, Kee-Yew Wong, Lawrence W. Stanton, Leonard Lipovich
Wayne State University Associated BioMed Central Scholarship
Abstract
Background
Transcriptional control of embryonic stem (ES) cell pluripotency has been a subject of intense study. Transcriptional regulators including Oct4 (Oct3/4 index), Sox2 and Nanog are fundamental for maintaining the undifferentiated state. However, the ES cell transcriptome is not limited to their targets, and exhibits considerable complexity when assayed with microarray, MPSS, cDNA/EST sequencing, and SAGE technologies. To identify novel genes associated with pluripotency, we globally searched for ES transcripts not corresponding to known genes, validated their sequences, determined their expression profiles, and employed RNAi to test their function.
Results
Gene Identification Signature (GIS) analysis, a SAGE derivative distinguished …