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Use Of Bioluminescent Imaging To Assay The Transplantation Of Immortalized Human Fetal Hepatocytes Into Mice., Moon Seok Choi, Andreea M Catana, Jian Wu, Young Seok Kim, Sang Jeong Yoon, Alexander D Borowsky, Sanjiv S Gambhir, Sanjeev Gupta, Mark A Zern
Use Of Bioluminescent Imaging To Assay The Transplantation Of Immortalized Human Fetal Hepatocytes Into Mice., Moon Seok Choi, Andreea M Catana, Jian Wu, Young Seok Kim, Sang Jeong Yoon, Alexander D Borowsky, Sanjiv S Gambhir, Sanjeev Gupta, Mark A Zern
jian duan wu
Noninvasive serial monitoring of the fate of transplanted cells would be invaluable to evaluate the potential therapeutic use of human hepatocyte transplantation. Therefore, we assessed the feasibility of bioluminescent imaging using double or triple fusion lentiviral vectors in a NOD-SCID mouse model transplanted with immortalized human fetal hepatocytes. Lentiviral vectors driven by the CMV promoter were constructed carrying reporter genes: firefly luciferase and green fluorescence protein with or without herpes simplex virus type 1 thymidine kinase. Human fetal hepatocytes immortalized by telomerase reconstitution (FH-hTERT) were successfully transduced with either of these fusion vectors. Two million stably transduced cells selected by …