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Full-Text Articles in Medicine and Health Sciences
From The Dinner Pot To Smoking Pot; How A Better Understanding Of Cannabidiol In Crayfish Could Alleviate Anxiety And Modulate Hunger, Christopher Michael Timmer
From The Dinner Pot To Smoking Pot; How A Better Understanding Of Cannabidiol In Crayfish Could Alleviate Anxiety And Modulate Hunger, Christopher Michael Timmer
Masters Theses
Anxiety affects approximately 1/3 of the US population and presents in many different forms, ranging from social to panic disorders. It also presents with high comorbidity for other mental disorders. One treatment is Selective Serotonin Reuptake Inhibitors (SSRIs) which allow for increased activation of serotonin (5-HT) receptors. SSRIs come with an extensive list of side effects, which can fail to maintain quality of life. Cannabidiol (CBD) is a cannabis derived compound which has been shown to decrease anxiety by activation of multiple subtype 5-HT amine receptors. CBD has few side effects, is not psychoactive, and exhibits anti-psychotic properties. The current …
Microbial Experience Influences Tumor- Infiltrating T Lymphocytes, Hanna Groeber
Microbial Experience Influences Tumor- Infiltrating T Lymphocytes, Hanna Groeber
Masters Theses
Immune cells, including T cells, have been used for anti-cancer therapy with varying degrees of success. One potential reason for immunotherapy failures in clinical trials may be that typical specific pathogen free (SPF) mice do not accurately replicate human microbial experience, which has important influence on shaping the adaptive immune response. Recently, several previous studies have shown that the immune system of SPF mice more closely resembles newborn human immunity, whereas immune systems from mice exposed to diverse pathogens more closely reflect adult human immunity.
To study the impact of microbial experience on the immune response, we have adopted a …
The Determination Of Phosphorylation Of Nato3 By Pka, Miranda M. Regenold
The Determination Of Phosphorylation Of Nato3 By Pka, Miranda M. Regenold
Masters Theses
One of the leading regulators of neuronal cell differentiation in the CNS is the family of basic helix–loop–helix (bHLH) transcription factors. One of these proteins, Nato3, is associated with the formation of dopaminergic neurons. Transcription factors can be regulated by kinase activity, and in order to detect the associated change of phosphorylation of Nato3, we have generated Nato3 with specific epitope tags that allow for the detection and isolation of the Nato3 protein. Through subcloning techniques and a successful transformation of the Nato3 gene with the sequences of the 3X-Flag epitope and the Myc epitope into the pcDNA3.0 vector, we …