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The Determination Of Tamoxifen Citrate In Human Blood Serum And Semen, Karimdad Tahmoresi
The Determination Of Tamoxifen Citrate In Human Blood Serum And Semen, Karimdad Tahmoresi
Chemistry & Biochemistry Theses & Dissertations
High performance liquid chromatography (HPLC)methodologies for the determination of tamoxifen and its metabolites 4-hydroxytamoxifen and n-desmethyltamoxifen, and using cis-tamoxifen as an internal standard have been developed.
Clinical analyses of tamoxifen and its metabolites in human serum and semen, were performed as follows: The analytes were extracted from serum and semen with hexane, the hexane evaporated, the dried extract then reconstituted with methanol, followed by reverse phase high performance liquid chromatography.
Between-run coefficients of variation (CV'S) for tamoxifen and n-desmethyltamoxifen for serum sample analyses, (n = 15) were, 4% and 13% at mean (SD) concentration of 140.2 (6.2) and 71.8 (9.8) …
A Batch Method For The Isolation Of Immunogenically Pure Igg, Iga, And Igm From Human Sera, Samuel Christopher Powell
A Batch Method For The Isolation Of Immunogenically Pure Igg, Iga, And Igm From Human Sera, Samuel Christopher Powell
Chemistry & Biochemistry Theses & Dissertations
A method is described for preparing an enriched fraction of the three major antibodies, immunoglobulins IgG, IgA and IgM, from normal human serum by a batch method. Selective precipitation of these proteins was carried out in successive steps using polyethylene glycol (PEG) of average molecular weight 6,000 at concentrations of 7%, 12% and 14% (16) . Zinc sulfate (10 mM) was used to remove highly immunogenic alpha2 macroglobulin from the IgM fraction. IgA, IgM and IgG were further purified by adsorption methods using DEAE-cellulose. Quantitation and qualitation of the enriched fractions showed nearly complete separation of each of the …