Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Cif Is Negatively Regulated By The Tetr Family Repressor Cifr, Daniel P. Maceachran, Bruce A. Stanton, George A. O'Toole
Cif Is Negatively Regulated By The Tetr Family Repressor Cifr, Daniel P. Maceachran, Bruce A. Stanton, George A. O'Toole
Dartmouth Scholarship
We previously reported that the novel Pseudomonas aeruginosa toxin Cif is capable of decreasing apical membrane expression of the cystic fibrosis transmembrane conductance regulator (CFTR). We further demonstrated that Cif is capable of degrading the synthetic epoxide hydrolase (EH) substrate S-NEPC [(2S,3S)-trans-3-phenyl-2-oxiranylmethyl 4-nitrophenol carbonate], suggesting that Cif may be reducing apical membrane expression of CFTR via its EH activity. Here we report that Cif is capable of degrading the xenobiotic epoxide epibromohydrin (EBH) to its vicinal diol 3-bromo-1,2-propanediol. We also demonstrate that this epoxide is a potent inducer of cif gene expression. We show that the predicted TetR family transcriptional …
Genetic Evidence For An Alternative Citrate-Dependent Biofilm Formation Pathway In Staphylococcus Aureus That Is Dependent On Fibronectin Binding Proteins And The Grars Two-Component Regulatory System, Robert M. Q. Shanks, Michael A. Meehl, Kimberly M. Brothers, Raquel M. Martinez, Niles P. Donegan, Martha L. Graber, Ambrose L. Cheung, George A. O'Toole
Genetic Evidence For An Alternative Citrate-Dependent Biofilm Formation Pathway In Staphylococcus Aureus That Is Dependent On Fibronectin Binding Proteins And The Grars Two-Component Regulatory System, Robert M. Q. Shanks, Michael A. Meehl, Kimberly M. Brothers, Raquel M. Martinez, Niles P. Donegan, Martha L. Graber, Ambrose L. Cheung, George A. O'Toole
Dartmouth Scholarship
We reported previously that low concentrations of sodium citrate strongly promote biofilm formation by Staphylococcus aureus laboratory strains and clinical isolates. Here, we show that citrate promotes biofilm formation via stimulating both cell-to-surface and cell-to-cell interactions. Citrate-stimulated biofilm formation is independent of the ica locus, and in fact, citrate represses polysaccharide adhesin production. We show that fibronectin binding proteins FnbA and FnbB and the global regulator SarA, which positively regulates fnbA and fnbB gene expression, are required for citrate's positive effects on biofilm formation, and citrate also stimulates fnbA and fnbB gene expression. Biofilm formation is also stimulated by several …