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Full-Text Articles in Medicine and Health Sciences
Development Of A Human Viable Heavy Chain Antibody Against The Alpha Folate Receptor As A Molecule For Targeted Application By Phage Display Technology, Nattihda Parakasikron
Development Of A Human Viable Heavy Chain Antibody Against The Alpha Folate Receptor As A Molecule For Targeted Application By Phage Display Technology, Nattihda Parakasikron
Chulalongkorn University Theses and Dissertations (Chula ETD)
Alpha folate receptor (FRα) is currently under investigation as a target for the treatment of patients with non-small-cell lung cancer (NSCLC), since it is highly expressed in tumor cells but is largely absent in normal tissue. In this study, a novel human variable domain of a heavy-chain (VH) antibody fragment specific to FRα was enriched and selected by phage bio-planning. The positive phage clone (3A102 VH) is specifically bound to FRα and also cross-reacted with FRβ, as tested by ELISA. Clone 3A102 VH was then successfully expressed as a soluble protein in an E. coli shuffle strain. The obtained soluble …
Designing An Extracorporeal Plasma Filtration Column With Specific Binding To Dengue Virions, Sasiwimon Thonghong
Designing An Extracorporeal Plasma Filtration Column With Specific Binding To Dengue Virions, Sasiwimon Thonghong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Dengue infection is one of the major public health concerns and no specific treatment is currently available. An extracorporeal system is one of the alternative treatment in severe dengue patients with impending shock. However, the commercial immunoadsorption devices were size-exclusive, non-selective designed for multipurpose thus not specific for dengue syndrome. This study focuses on establishing the flavivirus-specific (4G2) antibody entrapped into the alginate bead. Results showed that the viral titer reduced more than 90 % after 3 h running on a simulated circuit quantified by reverse transcription qPCR and plaque titration assay. The experiments were repeated with two biological replicates …
Endotoxemia From Nsaids-Induced Gut-Leakage Enhanced Lupus Characteristics In Fc Gamma Receptor Iib Deficient Lupus Mice, Thansita Bhunyakarnjanarat
Endotoxemia From Nsaids-Induced Gut-Leakage Enhanced Lupus Characteristics In Fc Gamma Receptor Iib Deficient Lupus Mice, Thansita Bhunyakarnjanarat
Chulalongkorn University Theses and Dissertations (Chula ETD)
High dose of non-steroidal anti-inflammatory drugs (NSAIDs), the common analgesia, might induce lupus activity through NSAIDs-induced gastrointestinal permeability defect (gut leakage) that causes endotoxemia. Indomethacin (25 mg/day) was orally administered for 7 days in 24-week-old Fc gamma receptor IIb deficient (FcgRIIb-/-) mice, an asymptomatic lupus model, and age-matched wild-type (WT) mice. The severity of indomethacin-induced enteropathy in FcgRIIb-/- mice was higher than WT mice as demonstrated by intestinal injury (histology, immune-deposition and intestinal cytokines), gut leakage (FITC-dextran assay and endotoxemia) and serum cytokines. In addition, higher responses against endotoxemia in indomethacin-administered FcgRIIb-/- mice was also supported by the prominent responses …
Impact Of Bacterial Dna In Severity Of Sepsis, Warerat Kaewduangduen
Impact Of Bacterial Dna In Severity Of Sepsis, Warerat Kaewduangduen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Bacterial sepsis can be caused by intestinal damage, leading to the passage of viable bacteria and their components into the circulatory system. Bacterial-free DNA in the blood during sepsis can be derived from bacterial death and intestinal translocation of free DNA in the gut contents. The presence of bacterial DNA in combination with lipopolysaccharide (LPS) can lead to more serious outcomes of sepsis. To explore the impact of bacterial-free DNA in sepsis, animal and macrophage experiments were performed. Firstly, the appearance of bacterial-free DNA and bacteriome in the blood was presented in mice with cecal ligation and puncture sepsis. Secondly, …
The Role Of Neutrophils Against Pythium Insidiosum, Apichaya Sriwarom
The Role Of Neutrophils Against Pythium Insidiosum, Apichaya Sriwarom
Chulalongkorn University Theses and Dissertations (Chula ETD)
Pythium insidiosum (P. insidiosum) is an oomycetes fungus-like microorganism causing pythiosis infection in humans. The zoospores (infective stage) of this pathogen contaminate aquatic environments such as moist soil and swampy area, which is a natural habitiat. Humans are infected with P. insidiosum via cutaneous route which is the main entry of the zoospores. Recent studies have shown that patients with pythiosis shows high morbidity and mortality rates particularly those with hematologic diseases. As neutrophils are the first line of immune defense during fungal infections, this study therefore investigated the activity of neutrophils in response to P. insidiosum. The neutrophil killing …
Mechanisms Of Colistin Resistance And The Antimicrobial Effects Of Antibiotic And Adjuvant Combination On Colistin-Resistant Klebsiella Pneumoniae, Aye Mya Sithu Shein
Mechanisms Of Colistin Resistance And The Antimicrobial Effects Of Antibiotic And Adjuvant Combination On Colistin-Resistant Klebsiella Pneumoniae, Aye Mya Sithu Shein
Chulalongkorn University Theses and Dissertations (Chula ETD)
The prevalence of colistin-resistant Klebsiella pneumoniae (ColRkp) has progressively increased globally. The purposes of this study were to characterize the molecular mechanisms responsible for colistin resistance among ColRkp clinical isolates in Thailand, to determine the expression levels of virulence factors associated with ColRkp clinical isolates, and to evaluate in vitro and in vivo synergistic activities of novel combination of antibiotic and adjuvant against ColRkp clinical isolates. A total of 165 carbapenem-resistant K. pneumoniae clinical isolates were obtained from King Chulalongkorn Memorial Hospital between 2016 and 2021. We discovered a rising trend of ColRkp displaying extensively drug-resistant (XDR) and pandrug-resistant (PDR) …
A Comparative Study Of Gut Microbiota In The Healthy Elderly And The Elderly With Colorectal Cancer, Nutta Iadsee
A Comparative Study Of Gut Microbiota In The Healthy Elderly And The Elderly With Colorectal Cancer, Nutta Iadsee
Chulalongkorn University Theses and Dissertations (Chula ETD)
Colorectal cancer is one of the third most common cancer worldwide. Dysbiosis of the human gut microbiota has been linked to sporadic colorectal cancer (CRC). This study aimed to compare the gut microbiota profile of a total of 80 Thai volunteers, who were above 50 years old, among 25 CRC patients, 33 adenoma patients, and 22 healthy controls (HC). The 16S rRNA sequencing was utilized to characterize the gut microbiome in both mucosal tissue and stool samples. Moreover, absolute quantitative PCR (qPCR) assay was conducted to quantify six CRC-associated bacteria including Fusobacterium nucleatum (FN), Parvimonas micra (PM), colibactin positive strains …
Comparative Response Of Dendritic Cells To Pathogenic And Non-Pathogenic Leptospira Spp., Pratomporn Krangvichian
Comparative Response Of Dendritic Cells To Pathogenic And Non-Pathogenic Leptospira Spp., Pratomporn Krangvichian
Chulalongkorn University Theses and Dissertations (Chula ETD)
Leptospirosis is a zoonotic disease caused by pathogenic Leptospira species, which consist of three groups and contain 4 subclades: pathogenic strains (P1), intermediate strains (P2), and non-pathogenic strains (S1 and S2). Infection of pathogenic leptospires occurs through contact with contaminated environments. Then, leptospires present in the bloodstream disseminate to target organs. Nowadays, the knowledge of the immune response to pathogenic and non-pathogenic leptospires during early infection is still limited. Due to the limitation of a susceptible host, immunological reagents for hamsters and strains of mice are limited and not available. Dendritic cells are the first cells to encounter leptospires found …
The Effect Of Xylitol And Erythritol In The Presence Of Sucrose On Single And Mixed-Species Biofilm Of Early Colonizer And Cariogenic Streptococci, Panadda Yuapibanrak
The Effect Of Xylitol And Erythritol In The Presence Of Sucrose On Single And Mixed-Species Biofilm Of Early Colonizer And Cariogenic Streptococci, Panadda Yuapibanrak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Dental caries is caused by multiple factors. An essential factor is sucrose. Dental caries is the result of an imbalance between early colonizers and cariogenic bacteria in biofilms. Sugar alcohols, xylitol and erythritol, become more popular because they do not cause cavities and are also effective at preventing caries. In everyday life, however, sucrose consumption is unavoidable. Thus, the aim of this study was to investigate the effect of xylitol and erythritol in the presence of sucrose on single- and mixed-species biofilm of early colonizers and cariogenic bacteria. Bacteria were cultured in broth containing 1% sucrose compared to broth containing …
The Synergistic Activity Of Antibiotic Combinations Against Colistin-Resistant Acinetobacter Baumannii Clinical Isolates And Its Mechanisms Related To Colistin Resistance, Sukrit Srisakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
The increasing development of colistin resistance in Acinetobacter baumannii clinical isolates is a global concern. Due to the limitation of effective antibiotics, the antibiotic combination therapies are the alternative choices. Here, we investigated the prevalence of colistin resistant A. baumannii (CoR-AB) clinical isolates and their resistance mechanisms, and examined the in vitro synergistic activities of the antibiotic combinations against CoR-AB. Among 341 carbapenem-resistant Acinetobacter spp., 317 (92.0%) were identified as A. baumannii by gyrB multiplex PCR. The rate of colistin resistance was 15.1% in A. baumannii isolates with MIC range of 0.125 – 64 mg/L. A total of 30 representative …