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Full-Text Articles in Medicine and Health Sciences
Application Of Phage Display Technology For The Production Of Antibodies Againststreptococcus Suis Serotype 2, Pattarawadee Sulong
Application Of Phage Display Technology For The Production Of Antibodies Againststreptococcus Suis Serotype 2, Pattarawadee Sulong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Streptococcus suis (S. suis) serotype 2 infection is problematic in the swine industry and responsible for most cases of human infection worldwide. Since current multiplex PCR cannot differentiate between serotypes 2 and 1/2, then serotype-specific antibodies (Abs) are required for serotype identification to confirm S. suis serotype 2 infection. This study aimed to generate Abs specific to S. suis serotype 2 by phage display from a human heavy chain variable domain (VH) antibody library. For biopanning, whole cells of S. suis serotype 2 were used as the target antigen. With increasing selection stringency, we could select the VH Abs that …
Molecular Detection Of Viable Mycobacterium Tuberculosis Complex In Clinical Specimens, Suthidee Petsong
Molecular Detection Of Viable Mycobacterium Tuberculosis Complex In Clinical Specimens, Suthidee Petsong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Tuberculosis (TB) is the global health concern because of the rising incidence and mortality. TB is caused by Mycobacterium tuberculosis complex (MTBC) and transmitted via inhalation of infectious droplet nuclei circulating in the air. The World Health Organization (WHO) recommends the use of Acid-fast (AFB) smear microscopy, MTBC culture, and molecular detection of MTBC DNA for diagnosis of TB. The response to treatment should be acquired as soon as possible to reduce disease transmission and drug resistance. Although MTBC culture is a gold standard, it is laborious, expensive, and may take up to 8 weeks. The AFB staining and molecular …
Detection Of Leptospires By Rpa-Nalfia And Crispr-Cas12a, Sirawit Jirawannaporn
Detection Of Leptospires By Rpa-Nalfia And Crispr-Cas12a, Sirawit Jirawannaporn
Chulalongkorn University Theses and Dissertations (Chula ETD)
The key barrier in leptospirosis diagnosis is a lack of available sensitive point-of-care testing. Therefore, we aimed to develop and validate nucleic acid lateral flow immunoassay (NALFIA) and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 12a (CRISPR/Cas12a) platform combined with isothermal amplification to detect leptospires from extracted patients' DNA samples. A recombinase polymerase amplification (RPA)-NALFIA and RPA-CRISPR/Cas12a assay was designed to detect the LipL32, SecY and lfb1 genes of pathogenic Leptospira spp. The RPA-NALFIA targeting LipL32 observed the LOD at 105 copies/reaction. In comparison, the RPA-CRISPR/Cas12a targeting LipL32 and SecY demonstrated a limit of detection (LOD) of 100 copies/reaction, …
The Influence Of Polycyclic Aromatic Hydrocarbons In Fcgriib -/- Lupus Mice, Kanyarat Udompornpitak
The Influence Of Polycyclic Aromatic Hydrocarbons In Fcgriib -/- Lupus Mice, Kanyarat Udompornpitak
Chulalongkorn University Theses and Dissertations (Chula ETD)
The activation of aryl hydrocarbon receptor (Ahr) through polycyclic aromatic hydrocarbons (PAHs), the major components of particulate matter at 2.5 micron (PM2.5) in the polluted air, might aggravate inflammation and lupus activity. Hence, 1,4-chrysenequinone (1,4-CQ), a substance in the PAHs group, was tested in a lupus model from FcgRIIb deficiency (FcgRIIb-/-) using macrophages and mice. Although the activation by 1,4-CQ alone was unable to induce inflammation, the pre-conditioning by lipopolysaccharide (LPS), a representative inflammatory-activator, before 1,4-CQ (LPS/1,4-CQ) induced more predominant inflammation in macrophages when compared with LPS or 1,4-CQ activation alone as determined by supernatant cytokines (TNF-α, IL-6 and IL-10). …
Synergistic Activities Of Antibiotic Combinations Against Carbapenem-Resistant Klebsiella Pneumoniae And Mechanisms Of Carbapenem Resistance In Klebsiella Pneumoniae Clinical Isolates., Netchanok Muhummudaree
Synergistic Activities Of Antibiotic Combinations Against Carbapenem-Resistant Klebsiella Pneumoniae And Mechanisms Of Carbapenem Resistance In Klebsiella Pneumoniae Clinical Isolates., Netchanok Muhummudaree
Chulalongkorn University Theses and Dissertations (Chula ETD)
Carbapenem-resistant Klebsiella pneumoniae (CRKP) has been increasing reported worldwide. The aims of this study were to investigate the mechanisms of carbapenem resistance in K. pneumoniae Thai isolates and study the synergistic activities of antibiotic combinations against CRKP isolates. Two-hundred and forty CRKP clinical isolates were obtained from patients at King Chulalongkorn Memorial Hospital between September 2016 and April 2020. The antibiotic susceptibility testing was determined by agar dilution to imipenem, meropenem, amikacin, fosfomycin, ciprofloxacin and by broth microdilution to colistin. The prevalence rates of resistance to imipenem, meropenem, ciprofloxacin, fosfomycin, amikacin, and colistin were 90.42%, 91.67%, 95.84%, 41.25%, 20%, and …
Role Of Chondrocyte Antigen Presentation In Osteoarthritis, Panjana Sengprasert
Role Of Chondrocyte Antigen Presentation In Osteoarthritis, Panjana Sengprasert
Chulalongkorn University Theses and Dissertations (Chula ETD)
Osteoarthritis (OA) is an inflammatory disorder characterized by degeneration of joint components, especially the cartilage. Chondrocytes are found in articular cartilage and play a role in cartilage production and degradation. Several studies found that chondrocytes not only control the cartilage metabolism, but also play a role in immune responses. However, the role of chondrocytes in the pathogenesis of OA is still unclear. In this study, we investigated the responses of OA chondrocytes after stimulating with IFN-γ and proteoglycan aggrecan and also determined antigen presentation function of chondrocytes to present proteoglycan aggrecan peptide to T cells. We found that OA chondrocyte …
Characterization Of Carbon Storage Regulator A (Csra) Homolog As A Global Gene Regulator In Leptospira Spp., Theerapat Phoka
Characterization Of Carbon Storage Regulator A (Csra) Homolog As A Global Gene Regulator In Leptospira Spp., Theerapat Phoka
Chulalongkorn University Theses and Dissertations (Chula ETD)
Leptospira spp. consist of non-pathogenic and pathogenic species. The bacteria can survive in diverse environments and therefore need mechanisms of rapid adaptation to environmental changes. However, molecular mechanisms of stress response in Leptospira are not fully understood. Carbon Storage Regulator A (CsrA) is a well-characterized, conserved, post-transcriptional global regulator that plays a critical role in response to environmental changes in many bacteria. CsrA has been reported to regulate several pathways such as glycogen metabolism, motility, biofilm formation, and virulence-associated genes. Based on available genomics data of Leptospira spp., we found putative csrA homolog, approximately 50% similarity with csrA of E. …
The Differential Dendritic Cell Properties In Response To Candida Albicans And Non-Albican Candida Β-Glucan, Truc Thi Huong Dinh
The Differential Dendritic Cell Properties In Response To Candida Albicans And Non-Albican Candida Β-Glucan, Truc Thi Huong Dinh
Chulalongkorn University Theses and Dissertations (Chula ETD)
Increased mortality and antifungal drug resistance of non-albicans Candidiasis patients have become an alarming in health problems nowadays, whereas profound knowledge of their pathogenesis mechanisms is still limited. The immune responses of innate and adaptive immunity play a crucial role in protecting from Candida infections. Dendritic cells (DCs) are the most notable because of their roles in bridging an innate immunity and specific adaptive immunity to eliminate fungal invasion. It is little known about DC functions in non-albicans Candida (NACs) infections. Besides, there is less study on evaluating the DC properties upon the interaction between DCs and NACs-derived ß-glucans. Therefore, …
Neutrophil Extracellular Traps (Nets) Formation Of Fcgr2b Deficient Mice In Lupus Mouse Model With Ischemic Reperfusion Injury, Wilasinee Saisorn
Neutrophil Extracellular Traps (Nets) Formation Of Fcgr2b Deficient Mice In Lupus Mouse Model With Ischemic Reperfusion Injury, Wilasinee Saisorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Acute kidney injury (AKI) caused by renal ischemic reperfusion (I/R) is the most prevalent cause of morbidity and mortality in patients. Neutrophil extracellular traps (NETs) are important for the progression of lupus nephritis after renal I/R injury. Because neutrophils are the first immune cell that respond to tissue damage, neutrophils might be an important cell that determines renal I/R injury. Additionally, NET components, including cell-free DNA, histone, nucleosome, and cytoplasmic protein compartment release the auto-antigens that accelerate lupus disease activity. Therefore, this project aimed to compare NETs formation in several internal organs following renal I/R injury in Fcgr2b deficient (Fcgr2b-/-) …
The Antiviral Activity Of Lichen Metabolites Against Dengue Virus, Naphat Loeanurit
The Antiviral Activity Of Lichen Metabolites Against Dengue Virus, Naphat Loeanurit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Dengue fever is one of the mosquito-borne infectious diseases globally. Currently, no specific treatment was established. Depsidones and depsides are one of the most common lichen secondary metabolites produced for the organism’s survival in extreme environments. These compounds have been reported as antivirals against the other virus in Flaviviridae family, hepatitis C virus. In this study, depsidones and depsides were explored for anti-dengue efficacy and cellular toxicity. One depsidone, VK-0014, and two depsides, TT-031 and TT-032, exhibited an anti-DENV2 with effective concentration (EC50) of 17.42 ± 3.21, 2.43 ± 0.19, and 0.91 ± 0.15 µM, respectively. Cytotoxicity concentration (CC50) in …