Medicine and Health Sciences Commons^™

Evaluation Of Stratified Antibiograms For Use In Laboratory And Antimicrobial Stewardship, Linsey Donner

Theses & Dissertations

Antibiograms are critical for choosing empiric antimicrobial therapy. Cumulative antibiograms, which aggregate susceptibility data, can mask differences within specific patient subsets or clinical syndromes. This dissertation was done to determine if antibiotic susceptibilities showed substantial differences when comparing stratified antibiograms to cumulative antibiograms.

Antibiotic susceptibility data was retrospectively obtained from Nebraska Medicine January 1, 2017 – December 31, 2019 for Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecalis. The University of Nebraska Medical Center’s web antibiogram clinical decision support tool was used to export the data. Bacteria-antibiotic susceptibility rates of stratified antibiograms …

Loss Of N1-Methylation Of G37 In Trna Induces Ribosome Stalling And Reprograms Gene Expression, Isao Masuda, Jae-Yeon Hwang, Thomas Christian, Sunita Maharjan, Fuad Mohammad, Howard Gamper, Allen R. Buskirk, Ya-Ming Hou

Department of Biochemistry and Molecular Biology Faculty Papers

N¹-methylation of G37 is required for a subset of tRNAs to maintain the translational reading-frame. While loss of m¹G37 increases ribosomal +1 frameshifting, whether it incurs additional translational defects is unknown. Here, we address this question by applying ribosome profiling to gain a genome-wide view of the effects of m¹G37 deficiency on protein synthesis. Using E coli as a model, we show that m¹G37 deficiency induces ribosome stalling at codons that are normally translated by m¹G37-containing tRNAs. Stalling occurs during decoding of affected codons at the ribosomal A site, indicating …

Molecular Interactions Of Human Cell Proteins With Sars-Cov-2 Versus Influenza Viruses, Linze Cowman

Honors Thesis

Molecular Interactions of Human Cell Proteins with SARS-CoV-2 versus Influenza

Viruses

Linze Cowman

Director: Victor Huber, Ph.D.

The novel Coronavirus Disease 2019, or COVID- 19, shows considerable similarity to a common influenza infection. Over the past year, innumerable studies have been investigating the Vitus in various capacities, from big-picture public health repercussions to the virus' biochemical interactions with human cells for infection. This research provides invaluable information in the ongoing war against infectious diseases. While the influenza virus is fairly well-understood, much surrounding the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains unclear, including its longevity, long-term health effects, methods …