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Two-Dimensional Fluorescence Difference Gel Electrophoresis Analysis Of The Urine Proteome In Human Diabetic Nephropathy, Kumar Sharma, Sohee Lee, Steven Han, Sungchun Lee, Barbara Francos, Peter Mccue, Richard Wassell, M. Alexander Shaw, Satish P. Ramachandrarao

Department of Medicine Faculty Papers

Urinary proteins may provide clues regarding pathogenesis of kidney disease as well as providing markers of disease activity. We employed two-dimensional differential in-gel electrophoretic analysis (2-D DIGE) to assess multiple urine samples in patients with diabetic nephropathy. Patient samples were collected as timed overnight collections. All the patients had longstanding diabetes, impaired renal function, and overt proteinuria. Control and patient urinary protein were analyzed by 2-D DIGE and DeCyder analysis. Ninety-nine spots were significantly regulated in the urine proteome of the diabetic samples, with 63 up- and 36 down-regulated. One spot corresponding to a pI 5–6 and a molecular weight …