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Articles 1 - 3 of 3
Full-Text Articles in Medicine and Health Sciences
Antisense Oligodeoxynucleotide Inhibition Of A Swelling-Activated Cation Channel In Osteoblast-Like Osteosarcoma Cells., Randall L. Duncan, Neil Kizer, Elizabeth L. Barry, Peter P A Friedman, Keith A. Hruska
Antisense Oligodeoxynucleotide Inhibition Of A Swelling-Activated Cation Channel In Osteoblast-Like Osteosarcoma Cells., Randall L. Duncan, Neil Kizer, Elizabeth L. Barry, Peter P A Friedman, Keith A. Hruska
Dartmouth Scholarship
By patch-clamp analysis, we have shown that chronic, intermittent mechanical strain (CMS) increases the activity of stretch-activated cation channels of osteoblast-like UMR-106.01 cells. CMS also produces a swelling-activated whole-cell conductance (Gm) regulated by varying strain levels. We questioned whether the swelling-activated conductance was produced by stretch-activated cation channel activity. We have identified a gene involved in the increase in conductance by using antisense oligodeoxynucleotides (ODN) derived from the alpha 1-subunit genes of calcium channels found in UMR-106.01 cells (alpha1S, alpha1C, and alpha1D). We demonstrate that alpha 1C antisense ODNs abolish the increase in Gm in response to hypotonic swelling following …
Distinct Cis-Acting Elements Mediate Clock, Light, And Developmental Regulation Of The Neurospora Crassa Eas (Ccg-2) Gene., Deborah Bell-Pedersen, Jay C. Dunlap, Jennifer J. Loros
Distinct Cis-Acting Elements Mediate Clock, Light, And Developmental Regulation Of The Neurospora Crassa Eas (Ccg-2) Gene., Deborah Bell-Pedersen, Jay C. Dunlap, Jennifer J. Loros
Dartmouth Scholarship
The Neurospora crassa eas (ccg-2) gene, which encodes a fungal hydrophobin, is transcriptionally regulated by the circadian clock. In addition, eas (ccg-2) is positively regulated by light and transcripts accumulate during asexual development. To sort out the basis of this complex regulation, deletion analyses of the eas (ccg-2) promoter were carried out to localize the cis-acting elements mediating clock, light, and developmental control. The primary sequence determinants of a positive activating clock element (ACE) were found to reside in a 45-bp region, just upstream from the TATA box. Using a novel unregulated promoter/reporter system developed for this study, we show …
Gtpase-Deficient G Alpha 16 And G Alpha Q Induce Pc12 Cell Differentiation And Persistent Activation Of Cjun Nh2-Terminal Kinases., Lynn E. Heasley, Brooke Storey, Gary R. Fanger, Laura Butterfield, J Zamarripa, D Blumberg, R A. Maue
Gtpase-Deficient G Alpha 16 And G Alpha Q Induce Pc12 Cell Differentiation And Persistent Activation Of Cjun Nh2-Terminal Kinases., Lynn E. Heasley, Brooke Storey, Gary R. Fanger, Laura Butterfield, J Zamarripa, D Blumberg, R A. Maue
Dartmouth Scholarship
Persistent stimulation of specific protein kinase pathways has been proposed as a key feature of receptor tyrosine kinases and intracellular oncoproteins that signal neuronal differentiation of rat pheochromocytoma (PC12) cells. Among the protein serine/threonine kinases identified to date, the p42/44 mitogen-activated protein (MAP) kinases have been highlighted for their potential role in signalling PC12 cell differentiation. We report here that retrovirus-mediated expression of GTPase-deficient, constitutively active forms of the heterotrimeric Gq family members, G alpha qQ209L and G alpha 16Q212L, in PC12 cells induces neuronal differentiation as indicated by neurite outgrowth and the increased expression of voltage-dependent sodium channels. Differentiation …