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- Acetylcysteine metabolism (1)
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Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Inhibition Of Glucocorticoid-Induced Apoptosis By Targeting Splice Variants Of Bim Mrna With Small Interfering Rna And Short Hairpin Rna., Marc T. Abrams, Noreen M. Robertson, Kyonggeun Yoon, Eric Wickstrom
Inhibition Of Glucocorticoid-Induced Apoptosis By Targeting Splice Variants Of Bim Mrna With Small Interfering Rna And Short Hairpin Rna., Marc T. Abrams, Noreen M. Robertson, Kyonggeun Yoon, Eric Wickstrom
Department of Biochemistry and Molecular Biology Faculty Papers
Glucocorticoids (GCs) induce apoptosis in lymphocytes and are effective agents for the treatment of leukemia. The activated glucocorticoid receptor (GR) initiates a transcriptional program leading to caspase activation and cell death, but the critical signaling intermediates in GC-induced apoptosis remain largely undefined. We have observed that GC induction of the three major protein products of the Bcl-2 relative Bim (BimEL, BimS and BimL) correlates with GC sensitivity in a panel of human pre-B acute lymphoblastic leukemia (ALL) cell lines. To test the hypothesis that Bim facilitates GC-induced apoptosis, we reduced BIM mRNA levels and Bim protein levels by RNA interference …
Hsp27 Anti-Sense Oligonucleotides Sensitize The Microtubular Cytoskeleton Of Chinese Hamster Ovary Cells Grown At Low Ph To 42 Degrees C-Induced Reorganization, Michael T. Hargis, C. W. Storck, Eric Wickstrom, Leonid A. Yakubov, Dennis B. Leeper, Ronald A. Coss
Hsp27 Anti-Sense Oligonucleotides Sensitize The Microtubular Cytoskeleton Of Chinese Hamster Ovary Cells Grown At Low Ph To 42 Degrees C-Induced Reorganization, Michael T. Hargis, C. W. Storck, Eric Wickstrom, Leonid A. Yakubov, Dennis B. Leeper, Ronald A. Coss
Department of Biochemistry and Molecular Biology Faculty Papers
Chinese hamster ovary (CHO) cells maintained in vitro at pH 6.7 were used to model cells in the acidic environment of tumours. CHO cells grown at pH 6.7 develop thermotolerance during 42 degrees C heating at pH 6.7 and their cytoskeletal systems are resistant to 42 degrees C-induced perinuclear collapse. Hsp27 levels are elevated in cells grown at pH 6.7 and are further induced during 42 degrees C heating, while Hsp70 levels remain low or undetectable, suggesting that Hsp27 is responsible for some of the novel characteristics of these cells. An anti-sense oligonucleotide strategy was used to test the importance …