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Comparison Of Some Kinetic Properties Of Pyruvate Kinase In Liver Tissue Of Normal And Diabetic Rats, Seval Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Diabetes mellitus was induced in Wistar-Albino type male rats with a single dose of streptozotocin (60 mg/kg intraperitoneally). The kinetic properties of pyruvate kinase were investigated in normal and diabetic rat liver tissue. In normal rat liver tissue, the incubation period of pyruvate kinase was 15 min and the optimum pH was 8. In diabetic rat liver tissue, the incubation period of pyruvate kinase was 10 min and the optimum pH was 7. Activation was found in normal and diabetic rat liver tissues with magnesium chloride and potassium chloride. Phospho(enol)pyruvate is an enzyme substrate; in the absence of the fructose-1,6-diphosphate …