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Full-Text Articles in Medicine and Health Sciences
Proopiomelanocortin Neurons In Nucleus Tractus Solitarius Are Activated By Visceral Afferents: Regulation By Cholecystokinin And Opioids, Suzanne M. Appleyard, Timothy W. Bailey, Mark W. Doyle, Young-Ho Jin, James L. Smart, Malcolm J. Low, Michael C. Andresen
Proopiomelanocortin Neurons In Nucleus Tractus Solitarius Are Activated By Visceral Afferents: Regulation By Cholecystokinin And Opioids, Suzanne M. Appleyard, Timothy W. Bailey, Mark W. Doyle, Young-Ho Jin, James L. Smart, Malcolm J. Low, Michael C. Andresen
Faculty Publications - Department of Biological & Molecular Science
The nucleustractus solitarius (NTS) receives dense terminations from cranial visceral afferents, including those from the gastrointestinal (GI) system. Although the NTS integrates peripheral satiety signals and relays this signal to central feeding centers, little is known about which NTS neurons are involved or what mechanisms are responsible. Proopiomelanocortin (POMC) neurons are good candidates for GI integration, because disruption of the POMC gene leads to severe obesity and hyperphagia. Here, we used POMC– enhanced green fluorescent protein (EGFP) transgenic mice to identify NTS POMC neurons. Intraperitoneal administration of cholecystokinin (CCK) induced c-fos gene expression in NTS POMC–EGFP neurons, suggesting that they …
Identification Of Neuronal Enhancers Of The Proopiomelanocortin Gene By Transgenic Mouse Analysis And Phylogenetic Footprinting, Flavio S. J. De Souza, Andrea M. Santangelo, Viviana Bumaschny, Marıa Elena Avale, James L. Smart, Malcolm J. Low, Marcelo Rubinstein
Identification Of Neuronal Enhancers Of The Proopiomelanocortin Gene By Transgenic Mouse Analysis And Phylogenetic Footprinting, Flavio S. J. De Souza, Andrea M. Santangelo, Viviana Bumaschny, Marıa Elena Avale, James L. Smart, Malcolm J. Low, Marcelo Rubinstein
Faculty Publications - Department of Biological & Molecular Science
The proopiomelanocortin (POMC) gene is expressed in the pituitary and arcuate neurons of the hypothalamus. POMC arcuate neurons play a central role in the control of energy homeostasis, and rare loss of function mutations in POMC cause obesity. Moreover, POMC is the prime candidate gene within a highly significant quantitative trait locus on chromosome 2 associated with obesity traits in several human populations. Here, we identify two phylogenetically conserved neuronal POMC enhancers designated nPE1 (600 bp) and nPE2 (150 bp) located approximately 10 to 12 kb upstream of mammalian POMC transcriptional units. We show that mouse or human genomic regions …