Medicine and Health Sciences Commons^™

Comparative Analysis Of Doxycycline And Ayurvedic Herbs To Target Metastatic Breast Cancer: An In-Silico Approach, Acharya Balkrishna, Rashmi Mittal, Rohan Malik, Hariom Verma, Kuldeep Singh Mehra, Hariom Chaturvedi, Okeshwar Okeshwar, Swami Ishdev, Ajay Kumar, Vedpriya Arya

BioMedicine

Background: Metastasis of breast cancer cells to distant sites including lungs, liver, lymph node, brain and many more have substantially affected the overall survival outcome and distant metastasis free survival rate amongst the diseased individuals. Several pre-clinical and clinical studies were carried out to determine the potency of vigorous inhibitors but they extensively deteriorated the patient’s quality of life. Hence, there exists an urgent need to explore potent natural remedy to fight against metastatic breast cancer.

Methods: Ayurvedic medicinal plants documented in literature for their ability to fight against breast cancer was screened and their respective active moieties were evaluated …

In Vitro And In Silico Evaluation Of Andrographis Paniculata Ethanolic Crude Extracts On 3 Fatty Acid Synthase Expression On Breast Cancer Cells, Nur Amanina Johari, Nur Anisa Sapi’I, Alvin Lu Jiunn Hieng, Nurriza Ab Latif, Syazwani Itri Amran, Rosnani Hasham, Khairunadwa Jemon

BioMedicine

Background Fatty acid synthase (FASN), a key rate-limiting enzyme in the fatty acid biosynthesis pathway has been identified to be overexpressed in breast cancer. This overexpression has been affiliated with poor prognosis and resistance to chemotherapeutics. Consequently, FASN has come into focus as an appealing potential target for breast cancer treatment. Available FASN inhibitors, however, are unstable and have been correlated with adverse side effects. Objective This present study aims to investigate the potential of Andrographis paniculata ethanolic crude extract (AP) as a potent FASN inhibitor in breast cancer cells. Materials & Methods This study used MTT assay and flow …

Zinc Oxide Nanoparticles-Doped Curcumin-Assisted Recovery Of Rheumatoid Arthritis And Antioxidant Status In Experimental Rabbits, Shanza Azeez, Mishal Fatima, Ouz Gul, Huzaifa Rehman, Muhammad Aslam Shad, Haq Nawaz

BioMedicine

Introduction: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by inflammation and synovial joint destruction.

Aims: The current study investigated the possible beneficial effect of zinc oxide nanoparticles-doped curcumin (ZnONPs-DC) on the recovery of RA and antioxidant status of experimental rabbits.

Methods: RA was induced in experimental rabbits by injecting complete Freund's adjuvant and collagen type-II emulsion (100 µL/kg body weight) in the base of their tail. Arthritic rabbits were orally treated with ZnONPs, curcumin, and ZnONPs-DC (250 µL/kg body weight). Serum samples from the control and study groups were collected before and after RA induction and after treatment. …

Protection Of Gueichih-Fuling-Wan On Cerebral Ischemia-Induced Brain Injury In Rodents Is Mediated By Trans-Cinnamaldehyde Via Inhibition Of Neuroinflammation And Apoptosis, Yuh-Fung Chen, Kuo-Jen Wu, Chi-Chung Kuo, Huei-Yann Tsai

BioMedicine

Background: Stroke is the leading cause of mortality and morbidity worldwide, and an effective therapeutic strategy for the prevention of patients with cerebral ischemiainduced brain injury is lacking. Traditional Chinese medicine with neuroprotective activities might be beneficial and provide alternative therapeutic opportunities for cerebral ischemia.Purposes: This study aimed to evaluate the neuroprotection and possible mechanisms of Gueichih-Fuling-Wan (GFW), its’ constitutive herbs, and their active compounds on cerebral ischemia/reperfusion (I/R)-induced brain injury in rodents.Methods: Various doses of extracts (0.25, 0.5, and 1.0 g/kg) of GFW and five constituent herbs (Cinnamomi Cortex, CC; Poria cocos, PC; Paeonia lactifloa, PL; Paeonia suffruticosa, PS …

Glutaminase - A Potential Target For Cancer Treatment, Josephine Anthony, Sureka Varalakshmi, Ashok Kumar Sekar, Nalini Devarajan, Balamurugan Janakiraman, Rajendran Peramaiyan

BioMedicine

The overexpression of glutaminase is reported to influence cancer growth and metastasis through glutaminolysis. Upregulation of glutamine catabolism is recently recognized as a critical feature of cancer, and cancer cells are observed to reprogram glutamine metabolism to maintain its survival and proliferation. Special focus is given on the glutaminase isoform, GLS1 (kidney type glutaminase), as the other isoform GLS2 (Liver type glutaminase) acts as a tumour suppressor in some conditions. Glutaminolysis linked with autophagy, which is mediated via mTORC1, also serves as a promising target for cancer therapy. Glutamine also plays a vital role in maintaining redox homeostasis. Inhibition of …

Unveiling The Power Of Flavonoids: A Dynamic Exploration Of Their Impact On Cancer Through Matrix Metalloproteinases Regulation, Peramaiyan Rajendran

BioMedicine

Cancer is a prominent contributor to mortality rates globally, with its progression and spread being the primary factors underlying this assertion. Despite advancements in cancer therapy, the existing therapeutic strategies are rendered useless as a result of their toxicity and the development of chemoresistance. Hence, it is imperative to assess alternate therapy modalities, and natural compounds present a promising prospect due to their demonstrated anticancer capabilities in several research models. This article provides an overview of the regulatory mechanisms involved in the expression of metalloproteinases (MMPs) and explores the potential therapeutic function of flavonoids as agents with anticancer activity that …

Phytochemical Composition Of Clinacanthus Nutans Based On Factors Of Environment, Genetics And Postharvest Processes: A Review, Nurul Hamizah Sabindo, Rusidah Mat Yatim, Kannan Thirumulu Ponnuraj

BioMedicine

Herpes simplex, varicella-zoster lesions, skin rashes, diabetes, snake bites and insect bites have all been treated by using Clinacanthus nutans (C. nutans). The pharmacological effects of C. nutans are influenced by the presence of terpenoids, flavonoids, alkaloids, phenolic acids, saponins, glycosides, steroids and tannins. This review focused on the phytochemical makeup, which varies geographically and is a subject of scarcely existing knowledge. C. nutans served as the primary search term, while the keywords “phytochemicals”, “chemical component” and “phytochemistry” were used to search the literature in the Google Scholar, PubMed, Scopus and Web of Science databases. The articles pertinent to the …

Vkorc1 Polymorphisms And Complete Resistance To Vitamin K Antagonists: About Two Cases., Ilham Benyamna, Houda El Fissi, Fadoua Bouzid, Abdelhamid El Mousadik, Najat Alif

BioMedicine

Complete resistance to vitamin K antagonists is a rare but serious. It can complicate therapeutic management, especially when direct oral anticoagulants cannot be used. Some single mutations in the VKORC1 gene have been identified in patients partially or completely resistant to vitamin K antagonists. We report the cases of two women in their fifties who presented an unexplained peripheral venous thrombosis. The aetiological assessment did not show any abnormalities. Genetic testing showed that both patients had the VKORC1 5417GG genotype. The VKORC1 3673 genotype was GG in case 1 and GA in case 2. The two patients showed complete resistance …

Computational Analysis Of Dimer G6pd Structure To Elucidate Pathogenicity Of G6pd Variants, Shamini Chandran, Naveen Eugene Louis, Syazwani Itri Amran, Nurriza Ab Latif, Muaawia Ahmed Hamza, Mona Alonazi

BioMedicine

An inherent genetic enzyme disorder in humans, known as glucose-6-phosphate dehydrogenase (G6PD) deficiency, arises due to specific mutations. While the prevailing approach for investigating G6PD variants involves biochemical analysis, the intricate structural details remain limited, impeding a comprehensive understanding of how different G6PD variants of varying classes impact their functionality. This study examined the dynamic properties of G6PD wild types and six G6PD variants from different classes using molecular dynamic simulation (MDS). The wild-type and variant G6PD structures unveil high fluctuations within the amino acid range of 274-515, the structural NADP⁺ binding site, pivotal for enzyme dimerization. Specifically, two …

Ethanol Leaf Extracts Of Anogeissus Leiocarpus In Antioxidants And Hepatotoxic Effects Of Escherichia Coli Infected Swiss Mice, Fred Coolborn Akharaiyi, Chioma Bertha Ehis-Eriakha, Peter Taiwo Olagbemide, Stephen Eromosele Akemu

BioMedicine

Introduction: Diseases caused by bacteria can be managed with medicinal plants with rightful dosage that will not affect body physiology and organs.

Aim: This research aimed to evaluate the antioxidants and the effects of Anogeisus leiocarpus on liver function.

Materials and Methods: Ethanol leaf extracts were processed for antioxidants and hepatotoxic effects using animal models. Group one (negative control) was given access to water and regular feed, group two (positive control) was dosed with 107 CFU/ml of Escherichia coli O157:H7, and groups 3-6 were dosed with 107 E. coli O157:H7 for 3 days and treated with extract concentrations of 12, …

Impact And Prevalence Of Comorbidities And Complications On The Severity Of Covid-19 In Association With Age, Gender, Obesity, And Pre-Existing Smoking: A Meta-Analysis, Soulandi Djorwé, Amale Bousfiha, Néhémie Nzoyikorera, Joseph Nyandwi, Bellamine Kawthar, Abderrahim Malki

BioMedicine

Background: COVID-19 patients usually present multiple comorbidities and complications associated with severe forms of SARS-CoV-2 infection. This study aimed to assess the risk factors and prevalence of comorbidities and complications contributing to the severity of COVID-19.

Methods: This meta-analysis was performed according to PRISMA guidelines. We searched various databases, including PubMed, Google Scholar, and Scopus (between 2020 and 2023), for eligible studies for this meta-analysis.

Results: Thirty-three studies were eligible, including 85812 patients, of which 36% (30634/85812) had severe disease, whereas 64% (55178/85812) had non-severe disease. Severe cases were potentially correlated with the following factors: gender (male) (odd ratio (OR) …

Biomedicines In Longevity And Aging The Quest To Resist Biological Decline, Raymond D D Raymond

BioMedicine

Aging is considered part of the natural process of life, however in recent years medical literature has started to show that specific facets of aging are beginning to be understood and those specific factors may even be considered preventable with various measures as this paper will demonstrate. Aging is also considered the number one cause of poor quality of life, disease, disability, and death, so the importance of understanding the aging process and how to control certain aspects of it cannot be underestimated when age related suffering is factored in. The causes of aging are now becoming well understood, and …

A Brief Literature Review Of Low-Level Laser Therapy For Treating Amyotrophic Lateral Sclerosis And Confirmation Of Its Effectiveness, Sergey Vladimirovich Moskvin

BioMedicine

Introduction: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with a steadily progressive course due to the death of central and peripheral motor neurons responsible for voluntary movements. Low-level laser therapy (LLLT) is a treatment method unique in its universality and efficacy, particularly for neurodegenerative diseases.

Methods: In this review, we discuss the effect and application of LLLT in the treatment of ALS. A literature search for English and Russian publications for the keywords "Amyotrophic Lateral Sclerosis", "Low-Level Laser Therapy" was performed using PubMed, Scopus, Google Scholar, Web of Science and Russian Science Citation Index (RSCI) databases.

Results: The article …