Medicine and Health Sciences Commons^™

Synaptotagmin C2b Ca2+-Binding Loops Impose Distinct Exocytosis Phenotypes, Michael W. Schmidtke

Wayne State University Theses

Regulated exocytosis from chromaffin cells in the adrenal medulla plays a critical role in maintaining organismal homeostasis. In the absence of stress, these cells release physiologically relevant substances into the blood stream only in limited quantities, whereas stressful conditions result in a rapid deluge of signaling molecules used, for example, to increase heart rate and pain tolerance. Although the cellular mechanisms governing the switch from low-level to stress-induced secretion are not well understood, recent evidence has implicated the exocytotic Ca2+-sensing protein Synaptotagmin (Syt) in this role.

Two isoforms of Syt are expressed in chromaffin cells (Syt-1 and Syt-7), and each …

Histological Evidence Of The Effect Of Oil Palm Phenolics In Atherogenic Diet Induced Rat Model Of Alzheimer’S Disease, Kenechukwu Monplaisir Monplaisir

Wayne State University Theses

BACKGROUND: Alzheimer’s disease (AD) is a neurodegenerative disease with the clinical presentation of memory loss and cognitive impairment. Alzheimer's disease pathology is the accumulation of beta amyloid plaques and neurofibrillary tangles.

METHOD: In this study atherogenic diet was used to induce AD in aged Brown Norway rats. The rats were assigned to the following four groups fed isocaloric diets; control group (C ),high cholesterol diet (H), high cholesterol + oil palm phenolics group (HP), high cholesterol + curcumin group (HC). The impact of oil palm phenolics (OPP) on neuronal health and its effect on amyloid deposition was evaluated using histology …

Characterization Of Oral Microbiota In Xerostomic Versus Non-Xerostomic Volunteers And In Daily Samples Following Standard Oral Hygiene Practices, Chelsea Myers

Wayne State University Theses

A thesis presented on the characterization of oral microbiota in xerostomic versus non-xerostomic volunteers and in daily samples following standard oral hygiene practices. Xerostomia is a difficult and burdensome disease that can be very difficult to diagnose. Understanding the oral microbiota between these diseased and healthy (non-xerostomic) can give us great insight on new treatments and/or prevention. Goals of the study included determining whether there substantial differences in oral microbial populations between the two groups, and whether varying nightly oral hygiene practices had an impact on next-morning oral microbiota titers or composition. Microbial loads were determined by qPCR using broad-spectrum …

Saudi Arabian Students In Detroit Use Natural Health Products More So Than Other University Students In Detroit, Samiah Naji Alqahtani

Wayne State University Theses

Natural Health Products (NHP), are defined as products that derive from organisms and may include supplements and herbal/traditional remedies. Prior research indicates that females use NHP more than males. We have unpublished data to show that students use NHP for health maintenance with significantly different use of NHP by Native/Aboriginal students vs. other students. Less is known about Saudi students. We hypothesize that: (1) There are gender differences in NHP used; and (2) There are geo-ethnic differences in NHP used. We also determine the predictor variables that influence NHP use by students. Students from Wayne State University (n=560; 193 …

Effect Of Folate Deficiency On Expression Of Proteins On The Mtor Signaling Pathway In The Brain Of C57/Bl6 Mice, Nikita Patel

Wayne State University Theses

ABSTRACT

EFFECT OF FOLATE DEFICIENCY ON EXPRESSION OF PROTEINS ON THE mTOR SIGNALING PATHWAY IN THE BRAIN OF C57/BL6 MICE

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NIKITA PATEL

December 2014

Advisor: Dr. Ahmad Heydari

Major: Nutrition and Food Science

Degree: Master of Science

Nutrient-gene interactions can significantly impact several cellular processes in the human body by altering important molecular pathways. Nutrition can affect brain structure, function and development throughout the life cycle; it can have a profound effect on cognition ad mental health leading to neurodegenerative diseases. It is important to elucidate the mechanisms by which diet can effect signaling pathways in the brain. The …

Effect Of Garcinol On Change In Gene Expression In Pancreatic Cancer Mouse Model, Nurul Huda Razalli

Wayne State University Theses

Pancreatic cancer is currently ranked as the fourth leading cause of cancer-related deaths in The United States making it so important to develop new approaches in order to improve the prognosis of this lethal disease. Chemotherapy drugs as treatment for pancreatic cancer are known to have adverse toxic reactions. Thus, development of treatments for pancreatic cancer that are relatively nontoxic is warranted. This thesis reports the potential anticancer effect of garcinol, a compound extracted from Garcinia indica fruit rind which may have promise for treating this lethal malignancy. Using KPC mouse-model for pancreatic cancer, this study has evaluated the mRNA …

The Effect Of Alpha-Cyclodextrin On Acute Blood Lipid And Glycemic Responses To A Fat Containg Meal, Evan Neil Fletcher

Wayne State University Theses

Obesity and hyperlipidemia have become major concerns in the United States over the past 30 years. Alpha-Cyclodextrin (á-CD), a naturally occurring soluble dietary fiber, has been shown to reduce dietary fat absorption and improve blood lipid levels in an animal model (mouse and rat) and in human studies. In the current double blind study, 34 healthy male and female participants were recruited to test if á-CD had any acute effect on blood lipid and glycemic responses to a fat containing meal. The participants received the á-CD on one occasion and a placebo the other to determine if there was any …

Studies Of Regulated Exocytosis From Neuroendocrine Cells, Madhurima Das

Wayne State University Theses

In this thesis we study cargo release and fusion pore dilation during calcium triggered exocytosis and the co-localization of calcium sensing proteins essential for exocytosis, in neuroendocrine cells.

Pancreatic beta cells secrete several hormones, the most studied one being insulin. C-peptide is a protein which is co-stored with and secreted from the same vesicles as insulin. It is found in the soluble phase unlike insulin, which is found in the dense core. The pancreatic beta cells also secrete the Chromogranin B (CgB) which is mostly found in the dense cores of secretory vesicles. In chapter 1, we found that CgB, …

Modulation Of Anti-Tumor Immune Response By Tgf-Β-Inducible Early Gene 1 (Tieg1), Andi Cani

Wayne State University Theses

Cancer immunotherapy has had limited clinical efficacy partly because regulatory T cells (Treg) suppress the immune response to tumor-associated antigens. Inducible regulatory T cells (iTreg), which are converted from naïve CD4 T cells by TGF-β, an abundant cytokine in the tumor microenvironment, may contribute to this immune suppression. Induction of Foxp3 by TGF-β is mediated by the transcription factor TIEG1 and abrogation of this protein prevents Foxp3 expression. We are testing the hypothesis that blockade of TIEG1 to prevent iTreg conversion will enhance immune response in DNA vaccination to the tumor associated antigen Her-2. Wild type and TIEG1 knockout mice …

The Use Of Metabolomics To Investigate Biomarker Profiles As Potential Early Risk Factors For Development Of Type Ii Diabetes Mellitus, Jennelle Lynn Arnew (Getty)

Wayne State University Theses

ABSTRACT

THE USE OF METABOLOMICS TO INVESTIGATE BIOMARKERS PROFILES AS POTENTIAL EARLY RISK FACTORS FOR DEVELOPMENT OF TYPE II DIABETES

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JENNELLE ARNEW

MAY 2012

Advisor: Dr. Smiti Gupta

Major: Nutrition and Food Science

Degree: Masters of Science

Type 2 Diabetes affects an estimated 17.5 million individuals in the United States and is considered to be one of the most pressing public health issues of our time. To date, the exact cause of type 2 diabetes remains unclear, however, it is considered to be an interplay between environmental and genetic factors. Emerging technologies for metabolomics analysis increases the capacity to …

Purification Of Alpha-Glucosidase Inhibitors From Grape Extract, Gayle M. Shipp

Wayne State University Theses

ABSTRACT

PURIFICATION OF ALPHA-GLUCOSIDASE INHIBITORS FROM GRAPE EXTRACT

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GAYLE SHIPP

August 2012

Advisor: Dr. Kevin Zhou

Major: Nutrition and Food Science

Degree: Master of Science

Diabetes and its complications are steadily growing and remain major causes of morbidity and mortality in the U.S. Intestinal á-glucosidases play a crucial role in controlling postprandial blood glucose. For this reason, one attractive prevention and/or treatment strategy for type-2 diabetes is the inhibition of á-glucosidase. The effectiveness of á-glucosidase inhibitors (AGI's) for diabetes treatment is well documented in numerous animal and human clinical studies. Grape pomace extract (GPE) has recently found in our …

The Effects Of Oil Palm Phenolics On Inflammation And Oxidative Stress In Relation To Amyloid Beta Plaques In Fad-Mutant And Wild Type B103 Cells, Lindsay Michelle Godsey

Wayne State University Theses

Alzheimer's disease (AD) affects approximately 35.6 million people in the world today (35). It is expected that by year 2050, an individual will develop AD every 33 seconds (8). Unfortunately, the etiology is not well known. Familial Alzheimer's disease (FAD) occurs due to specific genetic mutations, making it possible to replicate them in cells. Using B103 rat neuroblastoma cells transfected with a cDNA construct, wild type cells and FAD mutant cells were used in this research.

Natural ingredients such as oil palm phenolics (OPP) contain antioxidants. After finding the optimal experimental parameters, OPP was found to aid in the destabilization …

Effect Of Exogenous Leptin On Thrombotic And Metabolic Profiles Of Fvb/B6 Lipodystrophic Mice, Hoda Chaouki Kadouh

Wayne State University Theses

Lipodystrophy caused by fat deficiency contributes to metabolic disease for which several treatment modalities have been implemented, with leptin therapy being the most effective to date. In addition to playing a role in energy homeostasis and metabolism, leptin was also shown to play a pro-thrombotic role in mice. This role was not examined in fatless mice, neither was thrombosis measured. The AZIP/F-1 (FVB) lipodystrophic mouse appeared to have a prolonged arterial occlusion time (p〈0.05) in a trial done in our lab, with clotting factors being normal. The present study was designed to observe the thrombotic and metabolic characteristics of fatless …