Medicine and Health Sciences Commons^™

Efficacy Of A T Cell-Biased Adenovirus Vector As A Zika Virus Vaccine, Brianna L. Bullard, Brigette N. Corder, Matthew J. Gorman, Michael S. Diamond, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Zika virus (ZIKV) is a major public health concern due to the risk of congenital Zika syndrome in developing fetuses and Guillain-Barre syndrome in adults. Currently, there are no approved vaccines available to protect against infection. Adenoviruses are safe and highly immunogenic vaccine vectors capable of inducing lasting humoral and cellular immune responses. Here, we developed two Adenovirus (Ad) vectored Zika virus vaccines by inserting a ZIKV prM-E gene expression cassette into human Ad types 4 (Ad4-prM-E) and 5 (Ad5-prM-E). Immune correlates indicate that Ad5-prM-E vaccination induces both an anti-ZIKV antibody and T-cell responses whereas Ad4-prM-E vaccination only induces a …

Safety, Stephen M. Vantassel, Brenda K. Osthus

Wildlife Damage Management Technical Series

Wildlife damage management (WDM) is an exciting field with many opportunities to provide solutions to the complex issues involved in human-wildlife interactions. In addition, WDM wildlife control operators (WCO) face a variety of threats to their physical well-being. Injuries can result from misused (Figure 1), faulty, or poorly maintained equipment, inexperience, mishandled wildlife, harsh weather, and dangerous situations, such as electrical lines. The goals of this publication are to: * Develop an awareness of safety issues and adopt a mindset of “Safety First”, * Review the major safety threats that WCOs face, * Provide basic information for WCOs to protect …

The Impact Of Whoop Technology On Sleep, Recovery, And Performance In Naia Baseball Players, Nolan Harms

Department of Teaching, Learning, and Teacher Education: Dissertations, Theses, and Student Research

Many key factors – including but not limited to – sleep, nutrition, travel, stress, and practice influence the optimization of athletic performance. Although previous studies have investigated the use of wearable technology in sport to track several such factors, peer-reviewed research specific to WHOOP technology is limited at best. The purpose of this study was to examine the impact of WHOOP technology on sleep, recovery, and batting performance in healthy NAIA baseball players. Data was collected over the course of 4 weeks of in-season play on 10 varsity NAIA baseball players (20.40 ± 0.97 years). All games – 18 total …

Triatomines: Trypanosomatids, Bacteria, And Viruses Potential Vectors?, Caroline Barreto Viera, Yanna Reis Praça, Kaio Luis Da Silva Bentes, Paula Beatriz Santiago, Sofia Marcelino Martins Silva, Gabriel Dos Santos Silva, Flávia Nader Motta, Izabela Marques Dourado Bastos, Jaime Martins De Santana, Carla Nunes De Araújo

Harold W. Manter Laboratory: Library Materials

Triatominae bugs are the vectors of Chagas disease, a major concern to public health especially in Latin America, where vector-borne Chagas disease has undergone resurgence due mainly to diminished triatomine control in many endemic municipalities. Although the majority of Triatominae species occurs in the Americas, species belonging to the genus Linshcosteus occur in India, and species belonging to the Triatoma rubrofasciata complex have been also identified in Africa, the Middle East, South-East Asia, and in the Western Pacific. Not all of Triatominae species have been found to be infected with Trypanosoma cruzi, but the possibility of establishing vector transmission …

Triatomines: Trypanosomatids, Bacteria, And Viruses Potential Vectors? [Review], Caroline Barreto Vieira, Yanna Reis Praça, Kaio Luís Da Silva Bentes, Paula Beatriz Santiago, Sofia Marcelino Martins Silva, Gabriel Dos Santos Silva, Flávia Nader Motta, Izabela Marques Dorado Bastos, Jaime Martins De Santana, Carla Nunes De Araújo

Harold W. Manter Laboratory: Library Materials

Triatominae bugs are the vectors of Chagas disease, a major concern to public health especially in Latin America, where vector-borne Chagas disease has undergone resurgence due mainly to diminished triatomine control in many endemic municipalities. Although the majority of Triatominae species occurs in the Americas, species belonging to the genus Linshcosteus occur in India, and species belonging to the Triatoma rubrofasciata complex have been also identified in Africa, the Middle East, South-East Asia, and in the Western Pacific. Not all of Triatominae species have been found to be infected with Trypanosoma cruzi, but the possibility of establishing vector transmission …

Initiative 427: Nebraska Medicaid Expansion, J. David Aiken

Cornhusker Economics

This article summarizes information regarding Initiative 427–the Medicaid expansion question on the November 6, 2018 ballot. It reprints the actual ballot language and the Nebraska Secretary of State’s summary of arguments for and against Initiative 427.

Background. Originally Medicaid covered the elderly, the disabled, children in low-income families, and low-income pregnant women. In 2010 Medicaid coverage was expanded by Congress to include the working poor. In 2012 the U.S. Supreme Court ruled that the provision of the Affordable Care Act (ACA) requiring states to expand Medicaid was unconstitutional. This made it a state option whether or not to expand Medicaid. …

Kaposi’S Sarcoma-Associated Herpesvirus Seropositivity Is Associated With Type 2 Diabetes Mellitus: A Case–Control Study In Xinjiang, China, Meng Cui, Qiwen Fang, Jun Zheng, Zhanjun Shu, Yin Chen, Yage Fan, Juan Zhao, Charles Wood, Tiejun Zhang, Yan Zeng

Nebraska Center for Virology: Faculty Publications

Objective: To assess the potential relationship between Kaposi’s sarcoma-associated herpesvirus (KSHV) infection and type 2 diabetes mellitus (DM-2) in Xinjiang, China.

Methods: A case–control study of consecutively included DM-2 patients and normal controls was conducted among the Uygur and Han populations in Xinjiang Uygur Autonomous Region, China. Blood samples were collected and KSHV seroprevalence, antibody titers, and viral load were investigated. Logistic regression analysis and multiple linear regression analysis were applied to explore determinants of the main outcome measures.

Results: A total of 324 patients with DM-2 and 376 normal controls were included. The seroprevalence of KSHV was 49.1% (95% …

Gene Gangs Of The Chloroviruses: Conserved Clusters Of Collinear Monocistronic Genes, Phillip Seitzer, Adrien Jeanniard, Fangrui Ma, James L. Van Etten, Marc T. Facciotti, David D. Dunigan

Nebraska Center for Virology: Faculty Publications

Chloroviruses (family Phycodnaviridae) are dsDNA viruses found throughout the world’s inland waters. The open reading frames in the genomes of 41 sequenced chloroviruses (330 + 40 kbp each) representing three virus types were analyzed for evidence of evolutionarily conserved local genomic “contexts”, the organization of biological information into units of a scale larger than a gene. Despite a general loss of synteny between virus types, we informatically detected a highly conserved genomic context defined by groups of three or more genes that we have termed “gene gangs”. Unlike previously described local genomic contexts, the definition of gene gangs requires only …

Checklist Of Bloodfeeding Mites (Acari: Spinturnicidae) From The Wings Of Bats (Mammalia: Chiroptera) In The Manú Biosphere Reserve, Peru, Donald D. Gettinger

MANTER: Journal of Parasite Biodiversity

A survey collection of mites of the family Spinturnicidae from Peruvian bats includes 11 species of Periglischrus (acutisternus, gameroi, grandisoma, herrerai, hopkinsi, iheringi, micronycteridis, ojasti, paracutisternus, paravargasi, and ramirezi) and 2 Spinturnix (americanus and bakeri); almost all represent new locality records. This survey collection is available for further study at the following repositories: The Harold W. Manter Laboratory of Parasitology, University of Nebraska–Lincoln; the Field Museum of Natural History, Chicago; and the Laboratório de Espeleobiologia y Acarologia, Universidad Nacional Autónoma de México. When spinturnicid mites are …

Purification And Characterization Of Naturally Occurring Post-Translationally Cleaved Ara H 6, An Allergen That Contributes Substantially To The Allergenic Potency Of Peanut, Govardus A. H. De Jong, Shyamali Jayasena, Phil Johnson, Justin Marsh, Danijela Apostolovic, Marianne Van Hage, Julie Nordlee, Joe Baumert, Steve L. Taylor, Camille Roucairol, Harmen De Jongh, Stef J. Koppelman

Department of Food Science and Technology: Faculty Publications

The 2S albumin Ara h 6 is one of the most important peanut allergens. A post-translationally cleaved Ara h 6 (pAra h 6) was purified from Virginia type peanuts, and the cleavage site was mapped using high-resolution mass spectrometry. Compared to intact Ara h 6, pAra h 6 lacks a 5-amino acid stretch, resembling amino acids 43−47 (UniProt accession number Q647G9) in the nonstructured loop. Consequently, pAra h 6 consists of two chains: an N-terminal chain of approximately 5 kDa and a C-terminal chain of approximately 9 kDa, held together by disulfide bonds. Intermediate post-translationally cleaved products, in which this …

Comparison Of Maternal Beliefs About Causes Of Autism Spectrum Disorder And Association With Utilization Of Services And Treatments, Virginia Chaidez, Erik Fernandez Y Garcia, Lulu W. Wang, Kathleen Angkustsiri, Paula Krakowiak, Irva Hertz-Picciotto, Robin L. Hansen

Department of Nutrition and Health Sciences: Faculty Publications

Background: This study aimed to describe parental perceptions of the causes of autism spectrum disorder (ASD) in an ethnically diverse sample and explore whether these perceptions relate to treatment choices.

Methods: The sample consisted of White (n=224), Hispanic (n=85) and Asian (n=21) mothers of a child with ASD. A mixed methods approach was used in this secondary analysis focusing on parental perceptions about the causes of ASD and the relationship of these to utilization of services and treatment.

Results: Environmental and genetic factors were most often believed to be the cause or one of the causes of ASD by mothers …

Experimental Evaluation Of The Importance Of Colonization History In Early-Life Gut Microbiota Assembly, Inés Martínez, Maria X. Maldonado-Gomez, João Carlos Gomes-Neto, Hatem Kittana, Hua Ding, Robert J. Schmaltz, Payal Joglekar, Roberto Jiménez Cardona, Nathan L Marsteller, Steven W. Kembel, Andrew K. Benson, Daniel A. Peterson, Amanda Ramer-Tait, Jens C. Walter

Department of Food Science and Technology: Faculty Publications

The factors that govern assembly of the gut microbiota are insufficiently understood. Here, we test the hypothesis that inter-individual microbiota variation can arise solely from differences in the order and timing by which the gut is colonized early in life. Experiments in which mice were inoculated in sequence either with two complex seed communities or a cocktail of four bacterial strains and a seed community revealed that colonization order influenced both the outcome of community assembly and the ecological success of individual colonizers. Historical contingency and priority effects also occurred in Rag1^-/- mice, suggesting that the adaptive immune system …

Susceptibility Genes To Plant Viruses, Hernan Garcia-Ruiz

Nebraska Center for Virology: Faculty Publications

Plant viruses use cellular factors and resources to replicate and move. Plants respond to viral infection by several mechanisms, including innate immunity, autophagy, and gene silencing, that viruses must evade or suppress. Thus, the establishment of infection is genetically determined by the availability of host factors necessary for virus replication and movement and by the balance between plant defense and viral suppression of defense responses. Host factors may have antiviral or proviral activities. Proviral factors condition susceptibility to viruses by participating in processes essential to the virus. Here, we review current advances in the identification and characterization of host factors …

Individual And Community Quality Of Life In Nonmetropolitan Nebraska: Nebraska Rural Poll Research Report 18-3, Rebecca Vogt, Cheryl Burkhart-Kriesel, Randolph Cantrell, Brad Lubben, Larry J. Mcelravy Jr., Tim Meyer

Rural Futures Institute: Publications

Table of Contents: Executive Summary / Introduction / Trends in Community Ratings (1996 - 2018) / Figure 1 Community Change, 1996 - 2018 / Figure 2 Expected Community Change Ten Years from Now: 2011 – 2018 / Figure 3 Expected Destination of Those Planning to Move: 1998 - 2018 / Table 1 Proportions of Respondents Very or Somewhat Satisfied with Each Service, 1997 - 2018 / The Community and Its Attributes in 2018 / Figure 4 Perceptions of Community Change by Region / Figure 5 Expected Community Change in Ten Years by Community Size / Figure 6 Feelings of Community …

Social Issues In Nonmetropolitan Nebraska: Perceptions Of Social Stigma And Drug And Alcohol Abuse: 2018 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L. J. Mcelravy, Timothy L. Meyer

Rural Futures Institute: Publications

Conclusion

Many rural Nebraskans think it brings shame upon a person to do the following: file personal bankruptcy, apply for food stamps and apply for income assistance. Most rural Nebraskans disagree that the following items bring shame to a person: go to Alcoholics Anonymous or other alcohol treatment, seek mental health care, and go to drug treatment. In general, rural Nebraskans say that residents of their community are more likely than they personally are to think doing the items brings shame upon a person.

At least one-third of rural Nebraskans think the following are a very serious problem or a …

Establishing Hematology And Serum Chemistry Reference Intervals For Wild Hawaiian Monk Seals (Neomonachus Schauinslandi), Angela C. Kaufman, Stacie J. Robinson, Dori L. Borjesson, Michelle Barbieri, Charles L. Littnan

United States Department of Commerce: Staff Publications

Hematology and serum chemistry reference intervals have been previously established for the endangered Hawaiian monk seal (Neomonachus schauinslandi) as an imperative measure for health assessments. Monitoring the health of the wild population depends upon reference intervals that are context specific; hence we developed reference intervals from fresh samples, as opposed to frozen, from wild monk seals. This study builds on the number of parameters from previous efforts by using samples collected between 2004 and 2015 from wild monk seals. Blood samples were analyzed by a single veterinary diagnostic laboratory within 24 hr of collection from apparently healthy, wild …

Hyperthermia Induces Therapeutic Effectiveness And Potentiates Adjuvant Therapy With Nontargeted And Targeted Drugs In An In Vitro Model Of Human Malignant Melanoma, T. Mantso, S. Vasileiadis, I. Anestopoulos, G. P. Voulgaridou, E. Lampri, S. Botaitis, E. N. Kontomanolis, C. Simopoulos, G. Goussetis, R. Franco, K. Chlichlia, A. Pappa, M. I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

In the present study, we have aimed to characterize the intrinsic, extrinsic and ER-mediated apoptotic induction by hyperthermia in an in vitro model of human malignant melanoma and furthermore, to evaluate its therapeutic effectiveness in an adjuvant therapeutic setting characterized by combinational treatments with non-targeted (Dacarbazine & Temozolomide) and targeted (Dabrafenib & Vemurafenib) drugs. Overall, our data showed that both low (43 °C) and high (45 °C) hyperthermic exposures were capable of inducing cell death by activating all apoptotic pathways but in a rather distinct manner. More specifically, low hyperthermia induced extrinsic and intrinsic apoptotic pathways both of which activated …

Economic Development In Nonmetropolitan Nebraska: Internet Use, Nature-Based Tourism, Federal Tax Policy And Workforce Development: 2018 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L. J. Mcelravy, Timothy L. Meyer

Rural Futures Institute: Publications

Conclusion

The proportion of rural Nebraskans accessing the Internet using their cell phone has increased compared to two years ago. But, the proportion subscribing to high-speed Internet service at home remained stable during the past two years. Persons living in or near the smallest communities are less likely than persons living in or near larger communities to have high-speed Internet service at home.

Not many rural Nebraskans report significant limitations from their home Internet service on their ability to do most tasks. At least one in ten report being limited significantly or not being able to play real time video …

Preparation And Characterization Of Functionalized Heparin-Loaded Poly-Ɛ-Caprolactone Fibrous Mats To Prevent Infection With Human Papillomaviruses, Daniela Gonzalez, Jorge Ragusa, Peter C. Angeletti, Gustavo F. Larsen

Department of Chemical and Biomolecular Engineering: Faculty Publications

In this study, heparin-loaded poly-ε-caprolactone (PCL) fibrous mats were prepared and characterized based on their physical, cytotoxic, thermal, and biological properties. The main objective of the work described here was to test the hypothesis that incorporation of heparin into a PCL carrier could serve as bio-compatible material capable of inhibiting Human Papillomavirus (HPV) infection. The idea of firmly anchoring heparin to capture soluble virus, vs. a slow heparin release to inhibit a virus in solution was tested. Thus, one material was produced via conventional heparin matrix encapsulation and electrohydrodynamic fiber processing in one step. A second type of material was …

Pollen Evidence Of Medicine From An Embalming Jar Associated With Vittoria Della Rovere, Florence, Italy, Karl Reinhard, Kelsey B. Lynch, Annie Larsen, Braymond Adams, Leon Higley, Marina Milanello Do Amaral, Julia Russ, Donatella Lippi, Johnica J. Morrow, Dario Piombino-Mascali

Karl Reinhard Publications

Various samples of human viscera fragments, sponges, and cloth were collected from embalming jars belonging to members of the Medici family of Florence. One jar was labeled with the name Vittoria della Rovere, who died in March of 1694. This jar contained viscera fragments that were identified as a section of collapsed intestine. The intestine of the Vittoria della Rovere sample contained a large concentration of pollen belonging to the Myrtaceae family. The Myrtaceae pollen was sometimes observed in clusters during analysis, which is indicative of purposeful ingestion of flowers, buds, or a substance derived from floral structures. Thus, the …

Permission To Publish Agreement, Concepts In Animal Parasitology, University Of Nebraska-Lincoln Libraries

Concepts in Animal Parasitology Textbook

Blank Permission to Publish agreement form for authors of the Concepts in Animal Parasitology textbook/open educational resource.

Recovering Parasites From Mummies And Coprolites: An Epidemiological Approach, Morgana Camacho, Adauto Araújo, Johnica J. Morrow, Jane E. Buikstra, Karl Reinhard

Karl Reinhard Publications

In the field of archaeological parasitology, researchers have long documented the distribution of parasites in archaeological time and space through the analysis of coprolites and human remains. This area of research defined the origin and migration of parasites through presence/absence studies. By the end of the 20th century, the field of pathoecology had emerged as researchers developed an interest in the ancient ecology of parasite transmission. Supporting studies were conducted to establish the relationships between parasites and humans, including cultural, subsistence, and ecological reconstructions. Parasite prevalence data were collected to infer the impact of parasitism on human health. In the …

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Concepts in Animal Parasitology Textbook

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Information For Authors [Slides]: Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

Broadcast via Zoom live online, the following are the slides from an information session held for authors and reviewers of the Concepts in Animal Parasitology (working title: Animal Parasitology Concepts) textbook. The work will be written collaboratively and published in 2019 as an open educational resource under a CC BY-NC 4.0 International license.

Champs: Child Health And Methods Of Parenting Study, Megan Fletcher

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The range of different contexts in which children learn and develop dietary and physical activity habits varies greatly, yet there is limited research on the role of the family structure and parenting style in the development of childhood obesity. CHAMPS (Child Health and Methods of Parenting Study), was designed to explore the relationships between parenting style, family structure, and child health status through a new lens and novel application of the Unified Theory of Behavior model. Surveys were administered to families at two partner locations to examine these relationships. 51 parents/legal guardians and 57 children completed the survey. Results were …

References Style Guide, Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

References style guide for authors of the Concepts in Animal Parasitology free, online textbook.

Manuscript Preparation Guidelines, Concepts In Animal Parasitology, Paul Royster, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

Zea Books manuscript preparation guidelines for authors of the Concept in Animal Parasitology free, online textbook.

Instructions For Authors, Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

Instructions for authors for the Concepts in Animal Parasitology free, online textbook.

Template For Authors For Part Two (Endoparasites) And Part Three (Ectoparasites And Parasitoids), Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

Support material for authors of the open educational resource, Concepts in Animal Parasitology, Part 2 (Endoparasites) and Part 3 (Ectoparasites and Parasitoids).

Rapid Identification And Typing Of Mycobacterium Avium Complex Using Matrix Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (Maldi-Tof) Library And Biomarker Based Approaches., Claudia Antonika, John Dustin Loy, Raul Barletta, Michael Collins

UCARE Research Products

Bacteria associated with the Mycobacterium avium complex (MAC) cause significant problems in animal agriculture and cause large economic losses and morbidity of livestock. Two clinically important subspecies of M. avium are M.avium ssp paratuberculosis (MAP) and M. avium ssp hominissuis (MAH). MAP is the etiologic agent of Johne’s disease, a chronic and fatal enteritis in ruminants, that has been linked to Crohn’s disease in humans.1 MAH has zoonotic potential as it is one of the leading causes of secondary infections in AIDS patients. Identification of M. avium in clinical samples is challenging as they have overlapping host ranges and clinical …