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Life Sciences

TÜBİTAK

2020

In vitro

Articles 1 - 3 of 3

Full-Text Articles in Medicine and Health Sciences

Herbal Feed Additives Containing Tannins: Impact On In Vitro Fermentation And Methanemitigation From Total Mixed Ration, Manju Wadhwa, Prabh Kaur Sidhu, Mohinder Pal Singh Bakshi Jan 2020

Herbal Feed Additives Containing Tannins: Impact On In Vitro Fermentation And Methanemitigation From Total Mixed Ration, Manju Wadhwa, Prabh Kaur Sidhu, Mohinder Pal Singh Bakshi

Turkish Journal of Veterinary & Animal Sciences

Total mixed ration (TMR) containing roughage and concentrate mixture in a 65: 35 ratio on dry matter (DM) basis was supplemented with herbal feed additives [(HFAs); Acacia catechu (Katha), Areca catechu (Supari), and Acacia nilotica (Babul)] at 0-4% on DM basis to assess their impact on fermentation pattern and methane production by using in vitro gas production technique. Areca catechu had the highest (P < 0.01) concentration of condensed tannins (CTs), saponins, and vitamin C as compared to other HFAs. The net gas production (NGP), digestibility of NDF and true OM, and ME availability, partitioning factor (PF), volatile fatty acids (VFAs), and microbial biomass production were higher (P < 0.01) at 24 h as compared to t-half incubation, irrespective of type and level of HFAs supplemented. Acacia nilotica had an edge over Acacia catechu with respect to digestibility of nutrients and ME availability. VFAs production and efficiency of rumen fermentation was the highest from the Areca catechu-supplemented TMR. Irrespective of type of HFAs and incubation period, the digestibility of NDF and that of true OM were highest (P < 0.01) at the 1% level of supplementation, but depressed thereafter. Amongst HFAs-supplemented groups, VFA production and fermentation efficiency were highest at the 2% level. The results conclusively revealed that supplementing Areca catechu and Acacia nilotica at 2% of TMR (DM basis) inhibited the methane production, without affecting the fermentation pattern.


In Vitro Investigation Of Netosis Reaction Developing From Dog Polymorphonuclearneutrophils To Toxoplasma Gondii, Güneş Karakurt, Kader Yildiz Jan 2020

In Vitro Investigation Of Netosis Reaction Developing From Dog Polymorphonuclearneutrophils To Toxoplasma Gondii, Güneş Karakurt, Kader Yildiz

Turkish Journal of Veterinary & Animal Sciences

Toxoplasma gondii is known to develop extracellular traps from neutrophils in some animal species such as mice, cattle, sheep, cats, and donkeys. This study aimed to investigate the extracellular trap structures formed in dog polymorphonuclear leukocytes (PMNs) to T. gondii tachyzoites in vitro. Dog PMN was isolated using Percoll dilutions (45%, 54%, 63%, and 72%). After incubation with tachyzoites, the extracellular traps originating from the dog PMNs were observed in the extracellular areas. Histones (H3), myeloperoxidase (MPO), and neutrophil elastase (NE), the characteristic features of NETosis reaction, were detected in extracellular areas. The tachyzoites were observed between the extracellular trap …


Assessment Of The Molluscicidal Activity Of Wormwood (Artemisia Dubia, Wallich) Leaves Ethanolic Extract On Oncomelania Hupensis Quadrasi, Möllendorff, Claudine C. Terciño, Carl Leonard M. Pradera, Melvin A. Bagot Jan 2020

Assessment Of The Molluscicidal Activity Of Wormwood (Artemisia Dubia, Wallich) Leaves Ethanolic Extract On Oncomelania Hupensis Quadrasi, Möllendorff, Claudine C. Terciño, Carl Leonard M. Pradera, Melvin A. Bagot

Turkish Journal of Veterinary & Animal Sciences

This study assessed the in vitro molluscicidal activity of Artemisia dubia (wormwood) leaf ethanolic extract against adult and juvenile Oncomelania hupensis quadrasi and profiled its qualitative phytochemical content. The plants and snails were collected locally. There were 8 concentrations used for adult and juvenile snails: distilled water and 1% ethanol as negative controls; niclosamide (2 mg/L) as a positive control; and different concentrations of wormwood ethanolic extracts for adult snails including 3.98% (T1), 4.46% (T2), 5% (T3), 5.61% (T4), and 6.30% (T5) and for juvenile snails including 7.94% (T1), 8.91% (T2), 10% (T3), 11.22% (T4), and 12.59% (T5). Each treatment …