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Continuous Intrathecal Saline Infusion For Treating Refractory Spontaneous Intracranial Hypotension: A Case Report, Po-Fan Chiu, Yu-Hsiang Lin, Hui-Shan Lu, I-Han Hsiao, Hung-Lin Lin Nov 2023

Continuous Intrathecal Saline Infusion For Treating Refractory Spontaneous Intracranial Hypotension: A Case Report, Po-Fan Chiu, Yu-Hsiang Lin, Hui-Shan Lu, I-Han Hsiao, Hung-Lin Lin

BioMedicine

Spontaneous intracranial hypotension (SIH) is a poorly understood condition that presents with a wide variety of symptoms, ranging from mild headaches to coma. It is typically caused by continuous spontaneous leakage of spinal cerebrospinal fluid (CSF), resulting in orthostatic headaches. However, the appropriate management of refractory SIH remains unclear. A 50-year-old man presented with orthostatic headache followed by a rapid decline in mental status. The imaging findings were consistent with the diagnosis of SIH, with bilateral cerebral subdural hematomas and abnormal fluid collection in the posterior epidural space from the T2 to T12 levels. Computed tomography myelography of the whole …


Celastrol Restricts Experimental Periodontitis Related Alveolar Bone Loss By Suppressing Inflammatory Cytokine Response, Ahmet Altin, Meltem Zi̇hni̇ Korkmaz, Mehtap Atak, Tolga Mercantepe, Hülya Kiliç Yilmaz Nov 2023

Celastrol Restricts Experimental Periodontitis Related Alveolar Bone Loss By Suppressing Inflammatory Cytokine Response, Ahmet Altin, Meltem Zi̇hni̇ Korkmaz, Mehtap Atak, Tolga Mercantepe, Hülya Kiliç Yilmaz

BioMedicine

Introduction: Periodontitis is a common chronic inflammatory disease characterized by the destruction of the supporting structures of the teeth. The host defense mechanisms are responsible for inflamatuar and destructive reactions in periodontitis. Celastrol is one of the most promising components of the plant in Eastern and Southern China that has a long history of use in traditional medicine for the treatment of inflammatory conditions. Aim: The aim of this animal study was to inspect the preventive or restrictive effects of celastrol on periodontitis-related inflammatory host response and alveolar bone loss. Methods: 24 male Sprague Dawley rats were randomly assigned into …


Hematological Biomarkers Of Troponin, Neutrophil-To-Lymphocyte Ratio, And Monocyte-Tolymphocyte Ratio Serve As Effective Predictive Indicators Of High-Risk Mortality In Acute, Bryan Gervais De Liyis, Angela Faustine Ciaves, Marwa Humaira Intizam, Pierre Joshua Jusuf, I Made Junior Rina Artha Nov 2023

Hematological Biomarkers Of Troponin, Neutrophil-To-Lymphocyte Ratio, And Monocyte-Tolymphocyte Ratio Serve As Effective Predictive Indicators Of High-Risk Mortality In Acute, Bryan Gervais De Liyis, Angela Faustine Ciaves, Marwa Humaira Intizam, Pierre Joshua Jusuf, I Made Junior Rina Artha

BioMedicine

Background: Assessing high-risk mortality in acute coronary syndrome (ACS) patients, encompassing ST-Elevation Myocardial Infarction (STEMI), Non-ST-Elevation Myocardial Infarction (NSTEMI), and Unstable Angina Pectoris (UAP), is crucial. However, the prognostic significance of hematological parameters in predicting high-risk mortality in ACS patients remains uncertain despite advancements in ACS research. Aim: The aim was to investigate prognostic significance of hematological parameters troponin, Creatine Kinase-MB (CKMB), Neutrophil-to-Lymphocyte Ratio (NLR), Platelet-to-Lymphocyte Ratio (PLR), Monocyte-to-Lymphocyte Ratio (MLR), Basophil-to-Lymphocyte Ratio (BLR), and Eosinophil-to-Lymphocyte Ratio (ELR) levels in predicting high-risk mortality in ACS patients. Methods: In this retrospective observational study, data from medical records of 115 patients with …


Efficacy Of Hmj-38, A New Quinazolinone Analogue, Against The Gemcitabine-Resistant Mia-Paca-2 Pancreatic Cancer Cells, Mann-Jen Hour, Fuu‑ Jen Tsai, I-Lu Lai, Je-Wei Tsao, Jo-Hua Chiang, Yu-Jen Chiu, Hsing-Fang Lu, Yu‑ Ning Juan, Jai-Sing Yang, Shih-Chang Tsai Nov 2023

Efficacy Of Hmj-38, A New Quinazolinone Analogue, Against The Gemcitabine-Resistant Mia-Paca-2 Pancreatic Cancer Cells, Mann-Jen Hour, Fuu‑ Jen Tsai, I-Lu Lai, Je-Wei Tsao, Jo-Hua Chiang, Yu-Jen Chiu, Hsing-Fang Lu, Yu‑ Ning Juan, Jai-Sing Yang, Shih-Chang Tsai

BioMedicine

Gemcitabine is frequently utilized to treat pancreatic cancer. The purpose of our study was to create a gemcitabine-resistant MIA-PaCa-2 pancreatic cancer cell line (MIAGR100) and to evaluate the anti-pancreatic cancer efficacy of HMJ-38, a new quinazolinone analogue. Compared to their parental counterparts, MIA-PaCa-2, established MIA-GR100 cells were less sensitive to gemcitabine. MIA-GR100 cell viability was not affected by 10, 50, or 100 nM gemcitabine concentrations. HMJ-38 reduced MIA-GR100 cell growth and induced autophagy and apoptosis. When stained with monodansylcadaverine (MDC), acridine orange (AO), and terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL), MIA-GR100 cells shrunk, punctured their membranes, and produced …


Chemical Composition, Mineral Profile, Anti-Bacterial, And Wound Healing Properties Of Snail Slime Of Helix Aspersa Müller, Marouane Aouji, Amine Rkhaila, Bouchra Bouhaddioui, Malak Zirari, Hala Harifi, Youness Taboz, Lalla Aicha Lrhorfi, Rachid Bengueddour Nov 2023

Chemical Composition, Mineral Profile, Anti-Bacterial, And Wound Healing Properties Of Snail Slime Of Helix Aspersa Müller, Marouane Aouji, Amine Rkhaila, Bouchra Bouhaddioui, Malak Zirari, Hala Harifi, Youness Taboz, Lalla Aicha Lrhorfi, Rachid Bengueddour

BioMedicine

Mucus is a substance made by snails that serves a variety of purposes and is increasingly employed in the medical and cosmetic industries. It includes bioactive compounds with a range of biological characteristics that could be useful in the treatment of particular issues. This study assessed the wound-healing efficiency, antibacterial activity, chemical and mineral composition of Helix aspersa Müller slime. Inductively Coupled Argon Plasma Atomic Emission Spectrometry (ICP-OES) was used for mineral analysis, while Gas chromatography coupled to mass spectrometry (GC-MS) analysis was used for chemical characterization. The findings showed that the H. aspersa Müller slime had inhibitory activity on …


Mutation Patterns Of Integrase Gene Affect Antiretroviral Resistance In Various Non-B Subtypes Of Human Immunodeficiency Virus Type-1 And Their Implications For Patients’ Therapy, Fauzia Andrini Djojosugito, Arfianti Arfianti, Rudi , Wisaksana, Agnes Rengga Indrati Nov 2023

Mutation Patterns Of Integrase Gene Affect Antiretroviral Resistance In Various Non-B Subtypes Of Human Immunodeficiency Virus Type-1 And Their Implications For Patients’ Therapy, Fauzia Andrini Djojosugito, Arfianti Arfianti, Rudi , Wisaksana, Agnes Rengga Indrati

BioMedicine

Although primary integrase strand transfer inhibitor resistance mutations are currently uncommon, the increasing use of integrase strand transfer inhibitor as a key component of the first, second and third-line antiretroviral regimens suggests that the prevalence of integrase drug resistance mutations will likely increase. The rise of several polymorphic mutations and natural polymorphisms also affects the level of susceptibility of human immunodeficiency virus (HIV) type-1 to integrase strand transfer inhibitor. The considerable variability among the various subtypes of human immunodeficiency virus type-1 may contribute to differences in integrase mutations associated with integrase strand transfer inhibitors. Notably, non-B subtypes of HIV type-1 …


Combination Treatment Of Pembrolizumab With Dc-Cik Cell Therapy For Advanced Hepatocellular Carcinoma: A Case Report, Shao Min Huang, Long-Bin Jeng, Woei-Cherng Shyu, Hung-Yao Chen Aug 2023

Combination Treatment Of Pembrolizumab With Dc-Cik Cell Therapy For Advanced Hepatocellular Carcinoma: A Case Report, Shao Min Huang, Long-Bin Jeng, Woei-Cherng Shyu, Hung-Yao Chen

BioMedicine

Background: Recently, immunotherapy has emerged as a promising method for advanced HCC treatment. There are several clinical trials and meta-analyses of immune checkpoint inhibitors and immune cell therapy, but clinical evidence on the combination of these two therapies is lacking. Case description: A 66-year-old man with chronic hepatitis B-related cirrhosis complained of acute abdominal pain in an emergency department of a hospital. On exams, there was a palpable mass in the right upper quadrant of his abdomen. Contrast-enhanced abdominal computed tomography showed a large tumor in the right lobe, 13 cm x 17cm in size, and right portal vein thrombosis. …


Hipoxia Modulates The Secretion Of Growth Factors In Of Human Umbilical Cord-Derived Mesenchymal Stem Cells, Arfianti Arfianti, Ulfah Ulfah, Leopold Sampetua Hutabarat, Agnes Ivana G, Anisa Dwi Budiarti, Nabilla Sonia Sahara, Nicko Pisceski Kusika Saputra Aug 2023

Hipoxia Modulates The Secretion Of Growth Factors In Of Human Umbilical Cord-Derived Mesenchymal Stem Cells, Arfianti Arfianti, Ulfah Ulfah, Leopold Sampetua Hutabarat, Agnes Ivana G, Anisa Dwi Budiarti, Nabilla Sonia Sahara, Nicko Pisceski Kusika Saputra

BioMedicine

Background: Mesenchymal stem cell (MSC) has great potential as therapies due its ability to regenerate tissue damage and promote tissue homeostasis. Preconditioning of MSC in low oxygen concentration has been shown to affect the therapeutic potential of these cells. This study aimed to compare the characteristic and secretion of trophic factors of MSCs cultured under hypoxia and normoxia. Methods: MSCs were isolated from Wharton's jelly of human umbilical cord (UC) tissue by explant method and characterized by flow cytometry. Following 24 hours of CoCl2induced hypoxic culture, the viability and metabolic activity of MSC were analyzed by trypan blue exclusion test …


Evaluation Of Sars-Cov-2 Infection Risks After Primary Vaccination With Bnt162b2, Bbibp-Corv, Or Chadox1-Ncov-19 And After Homologous And Heterologous Booster Vaccinations With These Vaccines And Evaluation Of Sars-Cov-2 Reinfection Profiles, Soulandi Djorwé, Amale Bousfiha, Néhémie Nzoyikorera, Joseph Nyandwi, Bellamine Kawthar, Abderrahim Malki Aug 2023

Evaluation Of Sars-Cov-2 Infection Risks After Primary Vaccination With Bnt162b2, Bbibp-Corv, Or Chadox1-Ncov-19 And After Homologous And Heterologous Booster Vaccinations With These Vaccines And Evaluation Of Sars-Cov-2 Reinfection Profiles, Soulandi Djorwé, Amale Bousfiha, Néhémie Nzoyikorera, Joseph Nyandwi, Bellamine Kawthar, Abderrahim Malki

BioMedicine

Background: The emergence of SARS-CoV-2 variants has significantly increased the number of cases of COVID-19 among vaccinated individuals, raising concerns about the effectiveness of current vaccines. The aim of this study was to analyze the SARS-CoV-2 infection risks after primary vaccination with BNT162b2, BBIBP-CorV, or ChAdOx1-nCOV-19 and after homologues and heterologous booster vaccinations with these vaccines, as well as the profiles of reinfected patients. Methods: We analyzed retrospectively 1082 patients vaccinated or unvaccinated with BNT162b2, BBIBP-CorV, and/or ChAdOx1nCoV-19 vaccines to determine their SARS-CoV2 infection statuses using the reverse transcription-polymerase chain reaction (RT-PCR) in addition to their clinical features. The infection …


Evaluation Of Antibacterial Efficacy Of Lawsonia Inermis Linn (Henna) On Periodontal Pathogens Using Agar Well Diffusion And Broth Microdilution Methods: An In-Vitro Study, Şevki Güler, Damla Torul, Sevda Kurt-Bayrakdar, Emine Kübra Tayyarcan, Çağrı Çamsarı, İsmail Hakkı Boyacı Aug 2023

Evaluation Of Antibacterial Efficacy Of Lawsonia Inermis Linn (Henna) On Periodontal Pathogens Using Agar Well Diffusion And Broth Microdilution Methods: An In-Vitro Study, Şevki Güler, Damla Torul, Sevda Kurt-Bayrakdar, Emine Kübra Tayyarcan, Çağrı Çamsarı, İsmail Hakkı Boyacı

BioMedicine

Background: Although widely explored in medicine, limited evidence exists in the literature regarding the efficacy of Lawsonia inermis Linn (henna) in the dental field. Aim: This study aimed to investigate the antibacterial effect of henna on Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in vitro. Methods: The agar well diffusion and broth microdilution methods were used to evaluate the antibacterial effect of henna extracts. Dimethyl sulfoxide was used to prepare the ethanol extract of henna, and distilled water was used to prepare the water extract. For both ethanol and water extracts, 4 different concentrations were prepared as 15, 30, 60, and 120 …


Age-Specific Percentile-Based Prostate-Specific Antigen Cutoff Values Predict The Risk Of Prostate Cancer: A Single Hospital Observation, Teng-Fu Hsieh, Hung-Lin Chen, Ying-Fang Hsia, Che-Chen Lin, Hsiu-Yin Chiang, Min-Yen Wu, Sheng-Hsuan Chen, Po-Fan Hsieh, Hsi-Chin Wu, Han Chang, Chin-Chi Kuo Aug 2023

Age-Specific Percentile-Based Prostate-Specific Antigen Cutoff Values Predict The Risk Of Prostate Cancer: A Single Hospital Observation, Teng-Fu Hsieh, Hung-Lin Chen, Ying-Fang Hsia, Che-Chen Lin, Hsiu-Yin Chiang, Min-Yen Wu, Sheng-Hsuan Chen, Po-Fan Hsieh, Hsi-Chin Wu, Han Chang, Chin-Chi Kuo

BioMedicine

Background Testing for prostate-specific antigen (PSA) is often recommended for men with a potential risk of prostate cancer (PCa) before requiring advanced examination. However, the best PSA cutoff value remains controversial. Object We compared the predictive performance of age-specific percentile-based PSA thresholds with a conventional cutoff of >4 ng/mL for the risk of PCa. Methods We included men who received PSA measurements between 2003 and 2017 in a medical center in Taiwan. Logistic regression modeling was used to assess the association between age-specific percentile-based PSA thresholds and PCa risk in age subgroups. We further applied C-statistic and decision curve analysis …


Endometrial Fluid Biomarkers And Their Potential As Predictors Of Successful Embryo Implantation, Ana Rita Alves, Margarida Figueiredo Dias, Margarida Silvestre Aug 2023

Endometrial Fluid Biomarkers And Their Potential As Predictors Of Successful Embryo Implantation, Ana Rita Alves, Margarida Figueiredo Dias, Margarida Silvestre

BioMedicine

Background: Embryo implantation is a complex biological process which requires synchronized dialogue between the receptive endometrium and the blastocyst. The endometrium, however, is only receptive to embryo implantation for a very short period. Recurrent implantation failure (RIF) is a major challenge in assisted reproductive techniques mainly due to impaired receptivity, but there is still a need for a reliable and valid clinical test to assess endometrial receptiveness, especially at embryo transfer time. The aim of this review is to investigate what is currently known about the contribution of endometrial fluid (EF) to endometrial receptivity by identifying its potential biomarkers. Methods: …


The Intensity Of Free Radical Processes And Chaperone Activity In The Saliva Of Patients With Type 2 Diabetes, Anna A. Cheprasova, Sergey S. Popov, Alexander N. Pashkov, Aleksei N. Verevkin, Valentina O. Mittova, Konstantin K. Shulgin May 2023

The Intensity Of Free Radical Processes And Chaperone Activity In The Saliva Of Patients With Type 2 Diabetes, Anna A. Cheprasova, Sergey S. Popov, Alexander N. Pashkov, Aleksei N. Verevkin, Valentina O. Mittova, Konstantin K. Shulgin

BioMedicine

Introduction: Saliva is a clinically informative biological fluid that contains many biomarkers, allowing multiple analyses to be performed. Aim: The objectives of this study were the assessment of the serum and saliva levels of biochemical parameters and intensity of free radical processes in T2DM patients and the identification of the correlation between certain criteria. Methods: This case-control study included 40 T2DM patients, which were compared with 40 healthy individuals. The levels of glucose, cholesterol triglycerides, total protein, diene conjugates, and chaperone activity were measured using the spectrophotometric method. The concentration of 8-oxo-2'-deoxyguanosine was assessed by competitive enzyme-linked immunosorbent assay. Results: …


Radial Arterial Waves For Chemotherapy- And Radiotherapy-Related Myocardial Damage Identification In Patients With Breast Cancer, Chia-Ying Lee, Daniela Yunchin Yen, Mark C. Hou, Ying-Ling Chen, Rong-Jen Shiau May 2023

Radial Arterial Waves For Chemotherapy- And Radiotherapy-Related Myocardial Damage Identification In Patients With Breast Cancer, Chia-Ying Lee, Daniela Yunchin Yen, Mark C. Hou, Ying-Ling Chen, Rong-Jen Shiau

BioMedicine

Introduction

Chemotherapy and radiation therapy for breast cancer cause side effects, such as cardiovascular changes, which can be monitored with echocardiography. However, more convenient methods are always encouraged. Radial arterial waves that are used to detect cardiovascular changes can be used to assist in confirming cardiovascular changes.

Aim

This retrospective study aimed to analyze the frequency and time domains of the radial artery pulse wave in patients with breast cancer to understand its effectiveness in identifying cardiovascular changes.

Methods

Patients with breast cancer were screened from the pulse examination records in

Changhua Christian Hospital and divided into the treatment and …


Toxicological Evaluation Of A Polyherbal Formulation On Testicular Function And Gonadal Histomorphology In Exposed Wistar Rats, Godswill James Udom, Uduak Peter Ise, Omoirri Moses Aziakpono, Ayodeji Aturamu, Mba Ogbonnaya, Israel Kevin Umana, Jude Efiom Okokon May 2023

Toxicological Evaluation Of A Polyherbal Formulation On Testicular Function And Gonadal Histomorphology In Exposed Wistar Rats, Godswill James Udom, Uduak Peter Ise, Omoirri Moses Aziakpono, Ayodeji Aturamu, Mba Ogbonnaya, Israel Kevin Umana, Jude Efiom Okokon

BioMedicine

Introduction: Dr Iguedo Goko Cleanser® is a herbal formulation (HF) widely marketed in southern Nigeria and purported to be very efficacious for the management of various diseases including giardiasis, toilet infections, hypertension, diabetes, ulcer, impotence, low libido, low sperm count amongst others. Medicinal plants reportedly produce an array of adverse reactions capable of inducing harmful conditions, including death. Aim: This study evaluated the subchronic toxicity concern of HF on testicular function and gonadal histoarchitecture in Wistar rats. Methods: Thirty Wistar rats of both sexes were randomly divided into six groups (5/group) and were orally administered HF for 60 days. The …


Effects Of Anti-Retroviral Therapy On Baseline Serum Interleukin-18 Levels In Hiv-I Infected Patients Relative To Viral Suppression And Cd4+ Gain: A Prospective Pilot Study, Olayemi Balogun, Bukhari Isah Shuaib, Abdulrasheed Usman, Aminu Abba Yusuf, Bolanle O. P. Musa, Obiako O. Reginald, Aliyu Ahmadu Babadoko May 2023

Effects Of Anti-Retroviral Therapy On Baseline Serum Interleukin-18 Levels In Hiv-I Infected Patients Relative To Viral Suppression And Cd4+ Gain: A Prospective Pilot Study, Olayemi Balogun, Bukhari Isah Shuaib, Abdulrasheed Usman, Aminu Abba Yusuf, Bolanle O. P. Musa, Obiako O. Reginald, Aliyu Ahmadu Babadoko

BioMedicine

Background: In HIV infection, dysregulation of cytokines, including interleukin 18 (IL-18), has been linked to poor clinical outcomes in studies mainly conducted in resource-rich countries. This phenomenon has not been well-studied in resource-limited settings where outcomes could be confounded by exposure to endemic infections and genetic factors.

Objectives: Therefore, the influence of immunological and virological status of HIV-infected, antiretroviral therapy (ART)-naïve patients on serum IL-18 levels at baseline (pretreatment) and 24 weeks following initiation of combination ART (cART24) in a resource-limited setting was investigated. Methods Using the cross-sectional and longitudinal mixed method design, a total of Forty-four (44) newly diagnosed …


Metformin Induces Autophagy Of Cisplatin-Resistant Human Gastric Cancer Cells In Addition To Apoptosis, Chih-Wun Fang, Jai-Sing Yang, Jo-Hua Chiang, Po-Chuen Shieh, Fuu-Jen Tsai, Chia-Wen Tsai, Wen-Shin Chang May 2023

Metformin Induces Autophagy Of Cisplatin-Resistant Human Gastric Cancer Cells In Addition To Apoptosis, Chih-Wun Fang, Jai-Sing Yang, Jo-Hua Chiang, Po-Chuen Shieh, Fuu-Jen Tsai, Chia-Wen Tsai, Wen-Shin Chang

BioMedicine

Metformin has been used to treat cases of type 2 diabetes mellitus, and mounting studies have shown that metformin can act alone or in synergy with other anticancer agents to achieve anti-cancer efficacies on various types of tumors. However, the role of metformin in either inducing autophagy and cisplatin-resistance of human gastric cancer (GC) cells has never been examined. The study has established a cisplatin-resistant GC cell line and investigated the effects of metformin on inducing autophagy on it. The results demonstrated that treatment with metformin can concentration-dependently suppress the cell viability and cell confluence of cisplatin-resistant GC cells, while …


Precision Medicine Advancements In Glioblastoma: A Systematic Review, Karan Iyer, Shubham Saini, Suman Bhadra, Sohini Kulavi, Jaya Bandyopadhyay May 2023

Precision Medicine Advancements In Glioblastoma: A Systematic Review, Karan Iyer, Shubham Saini, Suman Bhadra, Sohini Kulavi, Jaya Bandyopadhyay

BioMedicine

Background. Glioblastoma multiforme, commonly known as GBM or glioblastoma is a grade IV astrocytoma. Brain tumors are difficult to treat and lead to poor prognosis and survival in patients. Gliomas are categorized into four different grades among which GBM is the worst grade primary brain tumor with a survival of less than a year. The genomic heterogeneity of the brain tumor results in different profiles for patients diagnosed with glioblastoma. Precision medicine focuses on this specific tumor type and suggests specialized treatment for better prognosis and overall survival (OS). Purpose. With the recent advancements in Genome-Wide Studies (GWS) and various …


Can Multidetector Ct Scan Replace Mri For Evaluation Of Mesorectal Fasca In Rectal Cancer?, Maryam Farghdani, Mehdi Karami, Amirafraz Fallah Feb 2023

Can Multidetector Ct Scan Replace Mri For Evaluation Of Mesorectal Fasca In Rectal Cancer?, Maryam Farghdani, Mehdi Karami, Amirafraz Fallah

BioMedicine

Abstract

Background

Colorectal cancer is the 4th leading cause of cancer mortality.

Aim

This study aimed to can multidetector CT scan (MDCT) replace Magnetic resonance imaging (MRI) for mesorectal fasca in rectal cancer?

Methods

This cross-sectional was conducted on patients with rectal cancer in Alzahra and Seyed-Shohada hospitals. MRI, mesorectal fascia (MPR), and axial MDCT were provided from patients to assess the involvement of mesorectal fascia. Sensitivity, specificity, positive and negative predictive values were assessed in these patients. Kappa coefficient agreement was used for analysis. Patients were also divided into different subgroups based on gender, tumoral location, nodal involvement, and …


A1 Adenosine Receptor Antagonist Induces Cell Apoptosis In Kyse-30 And Ym-1 Esophageal Cancer Cell Lines, Parisa Zeynali, Marie Saghaeian Jazi, Jahanbakhsh Asadi, Seyyed Mehdi Jafari Feb 2023

A1 Adenosine Receptor Antagonist Induces Cell Apoptosis In Kyse-30 And Ym-1 Esophageal Cancer Cell Lines, Parisa Zeynali, Marie Saghaeian Jazi, Jahanbakhsh Asadi, Seyyed Mehdi Jafari

BioMedicine

Background and Aim: Adenosine A1 receptor (AA1R) has been shown to have an inhibitory effect on cell growth in several cancers; however, its function in esophageal cancer is still unclear. In this study, we examined the effect of AA1R on cell growth and apoptosis in esophageal cancer cells. Materials and methods: In this study, YM-1 and KYSE-30 esophageal cancer cell lines were cultured. AA1R gene expression was determined by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). As well, the AA1R antagonist (DPCPX) effect on cell viability was evaluated by the MTT assay. Moreover, apoptosis was assessed by annexin-V and propidium iodide …


Jugular Foramen Versus Hypoglossal Canal In Axial Ct Scan, Maryam Mohammadzadeh, Reza Erfanian, Saman Rezaeian, Nasim Batavani, Behrooz Amirzargar Feb 2023

Jugular Foramen Versus Hypoglossal Canal In Axial Ct Scan, Maryam Mohammadzadeh, Reza Erfanian, Saman Rezaeian, Nasim Batavani, Behrooz Amirzargar

BioMedicine

Background: Differentiating jugular foramen from hypoglossal canal in computed tomography (CT) scan is vital for correct diagnosis of posterior fossa pathologies; however, it has been shown that the ability for differentiating these skull base elements is limited. The purpose of this study was to produce a simple algorithm for differentiating the jugular foramen from the hypoglossal canal in axial CT scan on two levels (top level where bony carotid canal is evident and lower level where bony carotid canal is not evident). Methods: Data derived from axial CT scan of 250 patients (500 sides) were used for producing algorithm. At …


The Impact Of Procalcitonin In Assessing Outcomes In Pediatrics Severe Trauma Cases: A Three-Year Experience From A Tertiary Hospital, Waleed H. Albuali Feb 2023

The Impact Of Procalcitonin In Assessing Outcomes In Pediatrics Severe Trauma Cases: A Three-Year Experience From A Tertiary Hospital, Waleed H. Albuali

BioMedicine

Background Although procalcitonin levels are raised in patients with systemic inflammation, its usage in pediatric patients, particularly those in the intensive care unit who are most susceptible to sepsis. Methods It is a retrospective research study that included pediatric patients aged more than two weeks who were brought to the King Fahd Hospital of the University's PICU owing to serious trauma or post-acute postoperative occurrences from January 2017 to December 2019. At 24 hours after admission, data such as age, gender, comorbidities, trauma severity as measured by the Injury Severity Score, and PRISM III score were collected. Results Following a …


High Levels Of Histone H3 K27 Acetylation And Tri-Methylation Are Associated With Shorter Survival In Oral Squamous Cell Carcinoma Patients, Akram Shahhosseini, Ekaterina Bourova-Flin, Samira Derakhshan, Pouyan Aminishakib, Afsaneh Goudarzi Feb 2023

High Levels Of Histone H3 K27 Acetylation And Tri-Methylation Are Associated With Shorter Survival In Oral Squamous Cell Carcinoma Patients, Akram Shahhosseini, Ekaterina Bourova-Flin, Samira Derakhshan, Pouyan Aminishakib, Afsaneh Goudarzi

BioMedicine

Background: Acetylation and trimethylation of histone H3 lysine 27 (H3K27ac and H3K27me3) generally activate and repress transcription, respectively. Concurrent activation of H3K27ac and H3K27me3 has been reported to correlate with poor prognosis in hepatocellular carcinoma. A high level of H3K27me3 has been shown to be associated with advanced oral squamous cell carcinoma (OSCC) tumour stage, but prognostic impact of H3K27ac level alone/or in combination with H3K27me3 in OSCC patients had not yet been reported. Material and Methods: In this study, immunohistochemistry using specific antibodies against H3K27ac and H3K27me3 was performed on a series of 72 OSCC samples to investigate the …


Effects Of Hepatocyte Growth Factor On Porcine Mammary Cell Growth And Senescence, Chi-Ping Huang, Liang-Chih Liu, Hsin-Ling Lu, Chih-Rong Shyr Feb 2023

Effects Of Hepatocyte Growth Factor On Porcine Mammary Cell Growth And Senescence, Chi-Ping Huang, Liang-Chih Liu, Hsin-Ling Lu, Chih-Rong Shyr

BioMedicine

Background: The porcine mammary glands share morphological and physiological similarities with human ones, making primary porcine mammary cells (PMC) suitable for biomedical research and a potential cellular therapeutic for breast cancer xenogeneic cell immunotherapy. Primary cells isolated from tissues remain the physiological functions of origin tissues but their self-renewal ability is restricted and cells acquire senescence during in vitro expansion. To overcome these drawbacks, here we sought to establish an approach to efficiently increase PMC’s in vitro growth. We studied the effects of the hepatocyte growth factor (HGF) to maintain the expansion capacity of porcine mammary cells and identify the …


New-Onset Diabetes In Covid-19: The Molecular Pathogenesis, Desak Made Wihandani, Made Lady Adelaida Purwanta, W. Riski Widya Mulyani, I Wayan Ardyan Sudharta Putra, I Gede Putu Supadmanaba Feb 2023

New-Onset Diabetes In Covid-19: The Molecular Pathogenesis, Desak Made Wihandani, Made Lady Adelaida Purwanta, W. Riski Widya Mulyani, I Wayan Ardyan Sudharta Putra, I Gede Putu Supadmanaba

BioMedicine

Diabetes mellitus (DM) is still a challenging metabolic disease worldwide. In the current situation, the world is facing a COVID-19 pandemic due to SARS-CoV-2 infection. DM is one of comorbid that can worsen the severity of the COVID-19 condition. Surprisingly, SARS-CoV-2 infection can induce new-onset diabetes, a condition in which acute hyperglycemia occurs and may potentially develop into a complication in non-diabetic patients. Angiotensin-converting enzyme 2 (ACE2) is a crucial entry factor of SARS-CoV-2 infection. ACE2 will bind to the spike protein of SARS-CoV-2, making its entrance to the potentially damaging process of many tissues in the human body, including …


Beyond Mental Stress-Induced Myocardial Ischemia Following The Covid-19 Vaccine, Chih-Cheng Lai, Po-Ren Hsueh Feb 2023

Beyond Mental Stress-Induced Myocardial Ischemia Following The Covid-19 Vaccine, Chih-Cheng Lai, Po-Ren Hsueh

BioMedicine

No abstract provided.