Medicine and Health Sciences Commons^™

Manipulation Of The Moloney Murine Leukemia Virus Envelope Protein In An Effort To Develop Directly And Indirectly Targeted Retroviral Vectors For Use In Human Gene Therapy, Geneva M. Vasser

Theses and Dissertations (ETD)

Highly effective, targeted therapies against cancer would revolutionize the way people recover from this devastating illness. Gone would be the lingering side effects of the current non-specific treatments and in their place would be faster recovery times, better quality of life both during and after treatment, and less ambiguity about whether or not treatment was effective. This concept will elude modern medicine until treatments can be tailored to the patient's individual and unique disease. This concept of a transient, targeted, and tailored vehicle aimed at cancer cells lends itself to the use of replication deficient retroviral gene therapy vectors with …

Regulation Of The Human Parainfluenza Virus (Hpiv3) Fusion Protein, Amanda Ruth Chapman

Theses and Dissertations (ETD)

Paramyxoviruses include a number of important human pathogens, including measles virus, mumps virus, and the human parainfluenza viruses (hPIV) 1-4, as well as several animal pathogens, such as Sendai virus, Nipah virus and Hendra virus. The creation of effective drugs and vaccines against this family of viruses would play an important role in decreasing the prevalence of these viruses and contributing to the health of both humans and animals worldwide. The purpose of this work was to determine how the fusion (F) protein is regulated with a focus on the heptad repeat B (HRB) region of the F protein located …

The Relationships Among Adaptive Behaviors Of Children With Autism Spectrum Disorder, Their Family Support Networks, Parental Stress, And Parental Coping, Heather Roberts Hall

Theses and Dissertations (ETD)

Purpose: The purpose of this study was to examine the adaptive behaviors of children with Autism Spectrum Disorder (ASD), their family support networks, parental stress, and parental coping and the relationships among these variables.

Background: Autism is the fastest growing diagnosed developmental disorder. When parents receive a diagnosis of autism for their child, mothers and fathers are affected differently. The costs to families of children with autism are personal, social, and affect family finances and day to day living. Parents of a child with autism are at high risk for increased stress levels. The child’s socially unacceptable behaviors and communication …

C-Reactive Protein Polymorphism And Serum Levels As An Independent Risk Factor In Sickle Cell Disease, Elizabeth A. Chismark

Theses and Dissertations (ETD)

This study explored the relationship of a dinucleotide repeat polymorphism in the intron of the CRP gene and serum CRP levels as independent risk factors for end-organ dysfunction (mild vs. severe) in adults with sickle cell disease. The pathogenesis of secondary complications of sickle cell disease is complex and poorly understood. Predicting the severity of these complications could assist in therapeutic decision-making.

The study measured serum CRP levels and the number of CA intron repeats located on the CRP gene in 29 adults (31.74 ± 11.54 years) with sickle cell disease The hemoglobin genotypes were distributed as Hgb SS 48.6% …

Communicable Disease And International Security: The Case Of Africa & Hiv/Aids, Amy L Rue

Archived Theses and Dissertations

In this thesis, I will look at how communicable disease fits into the human security discourse particularly with the disease being views as the 'potential threat' and the 'referent object' within the international sphere. The case of Africa will be used to illustrate the impact of HIV/AIDS on international security at country, regional and international levels. Ultimately, I will argue that communicable disease, as a human security issue, is a clear and important international security issue due to its status as a continual "potential threat" within international relations particularly in Africa. The elements that I will use to establish my …

The Role Of Msa In The Global Regulation Of Virulence In Staphylococcus Aureus, Vijayaraj Nagarajan

Dissertations

Staphylococcus aureus is an important pathogen causing life threatening diseases in humans. Previously we showed that msa modulates the activity of sarA (Staphylococcal accessory regulator), which is one of a major global regulator of virulence in S. aureus. The objective of this study is to characterize the role of msa (Modulator of SarA) in the global regulation of virulence in S. aureus. Structure and function predictions were done using several computational tools and approaches to understand the nature of msa. A novel S. aureus microarray meta-database (SAMMD) was designed and developed to compare and contrast other transcriptomes with msa transcriptome. …

Breast Cancer Screening Disparities In Japanese-American Women, Theodora Stratis

Loma Linda University Electronic Theses, Dissertations & Projects

Cancer screening disparities in ethnic minority women have received much research attention in the last several years due to a marked increase in ethnic minority cancer incidence and poor survival rates (Miller, Kollnel, Bernstein, Young Swanson, West et ah, 1996). Breast cancer is the leading cause of death for Asian American women (Asian American Network for Cancer Awareness, Research & Training, 2002) and, although their incidence and mortality rates are lower than Caucasian women, these rates continue to increase every year. This increasing mortality rate is likely due to the underutilization of breast cancer screening by Asian American women. Asian …

Characterization Of Kaposi's Sarcoma-Associated Herpesvirus Orf11 As A Possible Dutpase, Christina N. Ramirez

Seton Hall University Dissertations and Theses (ETDs)

A dUTPase is a crucial enzyme that hydrolyzes dUTP to dUMP. This reaction prevents the mutagenic or lethal misincorporation of uracil into DNA. For that reason, the enzyme is required for efficient DNA replication. Previous studies have shown that has ORFl l dUTPase-like motifs and thus may be a dUTPase. Generally, gammaherpesviruses contain six characteristic dUTPase motifs. In particular ORFl 1 and contains motifs 1, 2, 4, 6. While the characteristic motifs of gamroaberpesviruses include motifs 1, 2, 3, 4, 5, and 6, the number of dUTPase-like motifs in ORFl 1 's protein sequence is substantial. Thus, ORFl 1 may …

Hiv Risk Among Nursing Students In Rwanda : Self-Efficacy, Knowledge And Attitudes, Jacqueline Wosinski

Loma Linda University Electronic Theses, Dissertations & Projects

Acquired immune deficiency syndrome (AIDS) is the leading cause of death in sub-Saharan Africa. With a prevalence rate of 3.1% in 2006, Rwanda is one of the hardest hit countries. Nurses are often the leading health professionals in rural communities and at the forefront of the fight against the HIV pandemic. Little has been written on how nurses are prepared to intervene successfully along the continuum of HIV prevention and care. A mixed-methods sequential exploratory approach was used to explore this question.

In the first phase, cognitive, socio-psychological, and environmental factors of nursing education in Rwanda in regard to HIV …

Oxidative Stress-Mediated Anticancer Activity Of Novel Ahr Modulators Af & 5f203, Lancelot S. Mclean

Loma Linda University Electronic Theses, Dissertations & Projects

Estrogen receptor positive (ER+) breast cancer tends to respond to anti-estrogen agents such as Tamoxifen. Approximately 40% of ER+ breast cancer is resistant to these agents and those that initially respond often acquire resistance. Estrogen receptor negative (ER-) breast cancer remains largely unresponsive to these agents. It is therefore vital to discover drugs that are potent in both forms of breast cancer. Aminoflavone, (5-amino-2, 3-fluorophenyl)-6,8-difluoro-7-methyl-4H-l-benzopyran-4-one; AF; NSC 686288) and 5F203, (2-[-Amino-3-methy phenyl]-5-flurobenzothiazole) are novel anticancer candidate agents that display potent in vitro and in vivo anti-proliferative activity against select human tumor cells with a unique anticancer activity profile in the …

Novel Binding Domains Mediate Binding Of Hpv 16 E6 To Fadd And Procaspase 8, Sandy S. Tungteakkhun

Loma Linda University Electronic Theses, Dissertations & Projects

To evade the host response to infection, viruses have developed means to survive and propagate. HPV 16, a causative agent of cervical cancer and of some cases of oropharyngeal cancers, is one example. We have reported that the early viral protein E6 binds to proteins necessary for propagation of the apoptotic signal following receptor/ligand interactions, such as those mediated by FADD DED and procaspase 8 DED. E6 expression leads to the dose-dependent accelerated degradation of FADD and the protection of E6-expressing cells from Fas-induced apoptosis. Surprisingly, the splice isoforms of E6, E6_large and E6*, affect the stability of procaspase …

The Impact Of Being Born With Cleft And Cleft Reparative Surgery On Overall Health And Speech Outcomes, Khatansuudal Evsanaa

Master's Theses

Orofacial cleft is one of the most common and treatable birth defects in the world. If left untreated, orofacial cleft can impair normal speech development, growth, and could lead to a number of health consequences later in life. The main motivation of the study is to measure the impact of being born with cleft and the cleft reparative surgery on overall speech and health cleft for teenagers in India using difference-in-differences approach along with household fixed effects method. An overall health outcome was measured using height, weight, grip strength and BMI, and the speech acceptability was measured using a “Universal …

Transcriptional Regulation Of Azole Antifungal Resistance In Candida Albicans, Teresa T. Liu

Theses and Dissertations (ETD)

Candida albicans is a pathogenic fungi found in the mucosa, gastrointestinal, and urogenital tracts of humans. Oropharyngeal candidiasis (OPC), an opportunistic mucosal infection caused by C. albicans, occurs most frequently in patients infected with human immunodeficiency virus (HIV). OPC is usually treated with azole antifungals, a class of antifungals that target ergosterol biosynthesis, at low doses over long periods of time. This course of treatment allows for the development of azole resistance.

Two major mechanisms of azole resistance exist in C. albicans, the up-regulation of genes encoding efflux pumps and the up-regulation of ERG11, a gene encoding the azole drug …

Analysis Of The Cyc1 Promoter In Candida Albicans, Tomica Blocker

McCabe Thesis Collection

Scientists are considering two factors that may be important in the pathogenesis of C. albicans', it's capability to morph from yeast to hyphal phase, and its differences in colony morphology, cell shape, cell surface and cell permeability. (5) Unfortunately, unlike the bacteria, yeasts do not yet have proven virulence factors. However, science is suggesting that some of the major factors which contribute to the virulence of Candida are its ability to form hyphae, its ability to resist phagocytosis, its ability to adhere to epithelial cell surfaces, its ability to grow well at 37 degrees Celsius, and its ability to secrete …

Molecular Profile Of Women With And Without Secondary Breast Cancer After The Treatment Of Pediatric Hodgkin Lymphoma, Belinda Neal Mandrell

Theses and Dissertations (ETD)

dentification of genetic risk factors associated with the development of secondary cancers would facilitate identification of at risk patients and permit modification of therapy and heightened surveillance that may reduce cancer-related morbidity and mortality. Women survivors of pediatric Hodgkin lymphoma (HL) have an increased risk of morbidity and mortality associated with secondary effects of therapy, with a 35-75 fold excess risk of developing breast cancer over the general population. The mechanism for secondary breast cancer among Hodgkin survivors is not understood. Researchers have postulated that the familial characteristics of HL could be associated with mutations found within familial cancer syndromes; …

Biochemical And Immunological Mechanisms Underlying Differential Interaction Of Superantigens With Host Immunogenetic Factors In Streptococcal Sepsis, Mohammed Nooh

Theses and Dissertations (ETD)

Group A streptococci (GAS) are serious human pathogens that can cause a wide array of diseases ranging from pharyngitis to streptococcal toxic shock (STSS), which is caused by excessive cytokines responses triggered by streptococcal superantigens (Strep SAgs). SAgs interact simultaneously with HLA class II molecules on antigen presenting cells (APC) and with the T cell receptor beta chain variable elements (TCR V) and cause excessive stimulation of both cell types and massive release of inflammatory mediators.

The Strep SAgs include the streptococcal pyrogenic exotoxins (Spes), which play a crucial role in the pathogenesis of severe invasive streptococcal diseases, including Strep …

Sleep, Fatigue And Caregiver Burden In Parents Of Children With Acute Lymphoblastic Leukemia (All), Jerithea Tidwell

Theses and Dissertations (ETD)

Parents often report that their child diagnosed with acute lymphoblastic leukemia (ALL) and receiving steroid therapy experience altered sleep patterns, fatigue, irritability, and other behavioral changes. As a consequence, parents may experience altered sleep, fatigue, and increased caregiver burden. If so, the differences seen in parental sleep efficiency and sleep duration may be attributed to the child experiencing altered sleep secondary to receiving dexamethasone. The purpose of this study was to describe, caregiver sleep, fatigue, and caregiver burden immediately before and during their child’s dexamethasone pulse in continuation therapy for ALL and to compare parent quality of sleep and fatigue …

Time-Related Centile Ranges For Quality Of Life Outcomes In Renal Transplantation, Katy Garth

Theses and Dissertations (ETD)

The purpose of this study was to create time-related centile ranges that could be
used as benchmarks for evaluating and monitoring quality of life (QoL) following kidney transplantation. QoL is commonly viewed as an important indicator of successful outcomes in chronic disease including kidney transplantation. Despite extensive research documenting the value of QoL, routine measurement of QoL outcomes in clinical practice as a means of patient evaluation to augment clinical care has not been widely accepted. Lack of benchmarks for interpreting QoL scores may contribute to the reluctance to incorporate QoL measures into clinical care. The research question and study …

Predictors Of Depressive And Anxiety Symptoms Among African American Hiv-Positive Women, Nina Katherine Sublette

Theses and Dissertations (ETD)

This descriptive correlation study had four purposes: describe depressive and anxiety symptom levels in pregnant and non-pregnant African American (AA) HIV-positive women, examine the association between underlying vulnerabilities and depressive symptoms in HIV-positive AA women, determine the association between underlying vulnerabilities and anxiety symptoms in AA HIV-positive women, and determine the predictors of depressive and anxiety symptoms among African American HIV-positive women. A convenience sample of 80 African American HIV-positive women was recruited from university-based obstetrics/-gynecology and infectious disease practices in a large metropolitan city in the Mid-South. Depressive symptoms were measured with the Center for Epidemiologic Studies Depression Scale …

Application Of The Asset Mapping Model To Church Based Hiv/Aids Prevention And Control In Aba, Abia State, Nigeria, Godwin Nwadibia Aja

Loma Linda University Electronic Theses, Dissertations & Projects

This study examined whether HIV/AIDS activities were more frequent among churches with needs and assets for HIV/AIDS prevention and control in Aba, Nigeria and whether this connection was mediated by collective efficacy. A cross-sectional design was used to obtain data in two phases. First, a three-step forum focus group discussion (FFGD) method was used to obtain data from 32 representatives of five denominations. Second, a quantitative survey questionnaire was constructed based on the result of the FFGD and the collective efficacy model. This questionnaire was administered to 830 members aged 18 years and above from 83 churches that had operated …

An Assessment Of An Education Intervention On Youth Sports Coaches Knowledge Of Concussions, Heidi Elizabeth Brigham

Human Movement Sciences & Special Education Theses & Dissertations

Research has shown that sport related concussions are occurring at high rates across the United States. Research has also shown that there are many misconceptions among youth sports coaches regarding concussions. Therefore the purpose of this study was to determine the effects of an educational intervention on the knowledge of concussion recognition, management and prevention for youth sports coaches. A secondary purpose was to compare various relationships between demographic characteristics and the concussion knowledge assessment scores. A twenty-one question multiple choice assessment and 16 option symptom recognition checklist was utilized to assess concussion knowledge prior to and following an educational …

Evaluation Of The Efficacy Of Chloroplast-Derived Antigensagainst Malaria, Melissa Schreiber

Electronic Theses and Dissertations

Malaria is the most prevalent vector-borne parasitic disease worldwide and a major cause of death from infections. There is a great need to develop a low cost vaccine for malaria to control transmission of infection and impact of disease, due to the emergence of anti-malarial resistance. Two leading blood stage malarial vaccine candidates are the apical membrane antigen-1 (AMA-1) and the merozoite surface protein-1 (MSP-1). The aim of this project is to express malarial antigens in tobacco plants via plastid transformation and deliver them by subcutaneous or oral gavage of minimally processed transplastomic tissue to evaluate their efficacy to elicit …

Idiopathic Lesions And Visual Deficits In The American Lobster (Homarus Americanus) From Longs Island Sound, Ny, Christopher Robert Magel

Dissertations, Theses, and Masters Projects

In 1999 a mass mortality of the American lobster (Homarus americanus) occurred in western Long Island Sound (WLIS). Although the etiology remains unknown, warm bottom water temperature, hypoxia, heavy metal poisoning, and pesticides have been suggested as casual factors. Subsequently, lobsters from WLIS have continued to display symptoms of morbidity that include lethargy and cloudy grey eyes, caused by idiopathic lesions. The effects of these lesions on lobster vision are unknown. We therefore used electoretinography (ERG) to document changes in visual function in lobsters obtained from WLIS, while simultaneously using histology to quantify the extent of damage. Of the lobsters …

Cannabinoid Modulation Of Microglial Activation In Multiple Sclerosis, Natalie Shea Lemons

Honors Theses

Multiple Sclerosis (MS) is a neurodegenerative disease that is estimated to affect 400,000 Americans. There is no cure for MS and some treatments at this point are toxic to patients. Dr. Lori Hensley's lab is researching two drugs that could potentially be used to treat MS without toxic effects. Previous work in the lab shows ajulemic acid, a synthetic molecule similar to metabolites from the cannabis plant, selectively suppresses markers of the inflammatory response. worked on the mechanism of ajulemic acid's action by investigating the roles of several candidate receptors in mediating these observed effects.

Evaluation Of A Simple Intervention To Increase Self- Efficacy For Independent Exercise In Cardiac Rehabilitation Participants, Sherry A. Barkley

Electronic Theses and Dissertations

Purpose: While benefits of exercise after a cardiac event are well documented, participation in and adherence to cardiac rehabilitation (CR) programs is often low. The purpose of this study was to test the effectiveness of a self- efficacy coaching intervention (SCI): a simple theory-based behavioral intervention to increase self-efficacy for independent exercise as well as independent exercise behavior in CR patients. It was hypothesized that persons receiving the SCI treatment (T) would have higher levels of self-efficacy for exercise and greater participation in independent exercise than participants in an attention control (C) group.
Methods: People referred to a hospitalbased CR …

Resveratrol Modulation Of Microglial Activation And Provision For Neuroprotection In Multiple Sclerosis, Joseph L. Green

Honors Theses

Multiple sclerosis (MS) is an autoimmune disease affecting over 2.5 million people worldwide. In MS, myelin sheaths surrounding the axons of neurons are attacked by the immune system, leading to many deleterious symptoms. No cure for MS has been found, but some treatments reducing the number of exacerbations are available. Studies on resveratrol show the compound to have anti-inflammatory properties that could be beneficial in the treatment of diseases like MS. In our research, we used a cellular model to imitate neurons of the central nervous system and tested the effects of resveratrol in the hope of finding neuroprotective properties.