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Full-Text Articles in Medicine and Health Sciences
Development Of A Non-Invasive Dna Extraction Method From Teeth, Monalisa Heng
Development Of A Non-Invasive Dna Extraction Method From Teeth, Monalisa Heng
Honors Theses
Forensic dentistry has evolved from simple morphological investigations to genetic and molecular analysis. One of the most important techniques is DNA analysis which requires viable samples from an individual or crime site. The orofacial region can be a good source for DNA samples because the tissues can resist environmental, thermal, electrical, and mechanical challenges. Teeth are especially useful as a source of genetic material because the DNA located in the pulp is surrounded by protective mineral tissue. The DNA in the pulp tissue is currently accessed through destructive methods involving crushing, pulverization, and splitting the tooth. Unfortunately, destructive methods destroy …
A Nondestructive Procedure For The Extraction Of Dna From Human Teeth, Mark Reliquias, Ann-Elodie Black
A Nondestructive Procedure For The Extraction Of Dna From Human Teeth, Mark Reliquias, Ann-Elodie Black
Honors Theses
This research focuses on constructing a procedure that allows for the nondestructive extraction of mtDNA from teeth for forensic DNA analysis. Currently, only a destructive method for extracting DNA from teeth is used in the forensic laboratory to identify an individual. A disadvantage of extracting DNA through a destructive method is the complete or partial loss of the tooth. To extract DNA through a destructive method, the tooth must either be cut horizontally or vertically to access the pulp, or the root ground into a powder to extract DNA from the dentine-cementum. With the destruction of the tooth, additional forensic …