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Interaction Of Cationic Liposomes And Their Dna Complexes With Monocytic Leukemia Cells, Pedro Pires, Sérgio Simões, Shlomo Nir, Rogério Gaspar, Nejat Düzgüneş, Maria C. Pedroso De Lima
Interaction Of Cationic Liposomes And Their Dna Complexes With Monocytic Leukemia Cells, Pedro Pires, Sérgio Simões, Shlomo Nir, Rogério Gaspar, Nejat Düzgüneş, Maria C. Pedroso De Lima
All Dugoni School of Dentistry Faculty Articles
Cationic liposomes complexed with DNA have been used extensively as non-viral vectors for the intracellular delivery of reporter or therapeutic genes in culture and in vivo. We examined the relationship between the characteristics of the lipoplexes, their mode of interaction with monocytic THP-1 cells and their ability to transfect these cells. We determined the size and ζ potential of cationic liposomes (composed of 1,2-dioleoyl-3-(trimethylammonium) propane (DOTAP) and its mixtures with neutral lipids), and lipoplexes at different (+/-) charge ratios. As the (+/-) charge ratio of the lipoplexes decreased to (1/1), a significant reduction in ζ potential and an increase in …