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Full-Text Articles in Medicine and Health Sciences
Methodology For The Establishment Of Primary Porcine Vocal Fold Epithelial Cell Cultures, Elizabeth Erickson-Direnzo, Ciara Leydon, Susan L. Thibeault
Methodology For The Establishment Of Primary Porcine Vocal Fold Epithelial Cell Cultures, Elizabeth Erickson-Direnzo, Ciara Leydon, Susan L. Thibeault
Communication Disorders Faculty Publications
Objective: A current lack of methods for epithelial cell culture significantly hinders our understanding of the role of the epithelial and mucus barriers in vocal fold health and disease. Our first objective was to establish reproducible techniques for the isolation and culture of primary porcine vocal fold epithelial cells. Our second objective was to evaluate the functional significance of cell cultures using an in vitro exposure to an inflammatory cytokine. Methods: Epithelial cells were isolated from porcine vocal folds and expanded in culture. Characterization of cultures was completed by immunostaining with markers for pan-cytokeratin (epithelial cells), vimentin (stromal cells), von …
Epidermal Growth Factor Mediated Healing In Stem Cell-Derived Vocal Fold Mucosa, Liliana Palencia, Amritava Das, Sean P. Palecek, Susan L. Thibeault, Ciara Leydon
Epidermal Growth Factor Mediated Healing In Stem Cell-Derived Vocal Fold Mucosa, Liliana Palencia, Amritava Das, Sean P. Palecek, Susan L. Thibeault, Ciara Leydon
Communication Disorders Faculty Publications
Background: The goal of vocal fold wound healing is the reconstitution of functional tissue, including a structurally and functionally intact epithelium. Mechanisms underlying reepithelialization in vocal folds are not known, although it is suspected that healing involves the interplay between several growth factors. We used a three-dimensional human embryonic stem cell-derived model of vocal fold mucosa to examine the effects of one growth factor, exogenous epidermal growth factor (EGF), on wound healing. Materials and methods: A scratch wound was created in the in vitro model. Rate of wound healing, epidermal growth factor receptor (EGFR) activation, and cell proliferation after injury …
Vocal Fold Epithelial Barrier In Health And Injury: A Research Review., Elizabeth E. Levendoski, Ciara Leydon, Susan L. Thibeault
Vocal Fold Epithelial Barrier In Health And Injury: A Research Review., Elizabeth E. Levendoski, Ciara Leydon, Susan L. Thibeault
Communication Disorders Faculty Publications
Purpose: Vocal fold epithelium is composed of layers of individual epithelial cells joined by junctional complexes constituting a unique interface with the external environment. This barrier provides structural stability to the vocal folds and protects underlying connective tissue from injury while being nearly continuously exposed to potentially hazardous insults, including environmental or systemic-based irritants such as pollutants and reflux, surgical procedures, and vibratory trauma. Small disruptions in the epithelial barrier may have a large impact on susceptibility to injury and overall vocal health. The purpose of this article is to provide a broad-based review of current knowledge of the vocal …
Structural And Functional Vocal Fold Epithelial Integrity Following Injury, Ciara Leydon, Mitsuyoshi Imaizumi, David T. Yang, Susan L. Thibeault, Marvin Fried
Structural And Functional Vocal Fold Epithelial Integrity Following Injury, Ciara Leydon, Mitsuyoshi Imaizumi, David T. Yang, Susan L. Thibeault, Marvin Fried
Communication Disorders Faculty Publications
Objectives/Hypothesis: An intact epithelium is an important part of vocal fold defense. Damage to the epithelium can compromise vocal fold homeostasis and protection of the host tissue from viral and bacterial invasion. Elucidating the effects of damage on epithelial architectural and barrier integrity provides insight into the role of epithelium in protecting vocal folds. Using an animal model, we evaluated the time course of structural and functional epithelial restoration following injury. Study Design: Prospective, controlled animal study. Methods: Forty rats underwent surgery to remove vocal fold mucosa unilaterally. Larynges were harvested at five time intervals between 3 to 90 days …