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Medicine and Health Sciences Commons

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Chemicals and Drugs

1990

Cerevisiae

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A Rapid And Simple Method For Preparation Of Rna From Saccharomyces Cerevisiae, Mark E. Schmitt, Timothy A. Brown, Bernard L. Trumpower Mar 1990

A Rapid And Simple Method For Preparation Of Rna From Saccharomyces Cerevisiae, Mark E. Schmitt, Timothy A. Brown, Bernard L. Trumpower

Dartmouth Scholarship

Most methods for isolation of RNA from yeast require tedious vortexing with glass beads, and give low yields when scaled down to 10 ml cultures (1). In addition, it is frequently desirable to prepare RNA from several different yeast strains grown under a variety of growth conditions, and preparations using glass beads are impractical when dealing with multiple samples.