Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Adjuvants (1)
- Animals (1)
- Antibodies (1)
- Antioxidative activity (1)
- BOl (1)
-
- Bacterial (1)
- Bacterial outer membrane proteins (1)
- Cholera (1)
- Cholera vaccines (1)
- Chondrocytic cells (1)
- Collagenase-3 (1)
- Dehydroepiandrosterone (1)
- Dextran‐methylprednisolone (1)
- Dll EA (1)
- Female (1)
- Fimbriae (1)
- Fimbriae proteins (1)
- Il-1 (1)
- Immunologic (1)
- Jnk (1)
- Lethal dose 50 (1)
- Macromolecular prodrug (1)
- Methylprednisolone (1)
- Mice (1)
- Microbial immunity and vaccines (1)
- Newborn (1)
- P38 mapk (1)
- Peptide fragments (1)
- Pharmacokinetics (1)
- Polymers (1)
Articles 1 - 6 of 6
Full-Text Articles in Medicine and Health Sciences
Evaluation Of Cholera Vaccines Formulated With Toxin-Coregulated Pilin Peptide Plus Polymer Adjuvant In Mice, Jia-Yan Wu, William F. Wade, Ronald K. Taylor
Evaluation Of Cholera Vaccines Formulated With Toxin-Coregulated Pilin Peptide Plus Polymer Adjuvant In Mice, Jia-Yan Wu, William F. Wade, Ronald K. Taylor
Dartmouth Scholarship
Cholera is an acute diarrheal disease that is caused by the gram-negative bacterium Vibrio cholerae. The low efficacy of currently available killed-whole-cell vaccines and the reactinogenicity coupled with potential reversion of live vaccines have thus far precluded widespread vaccination for the control of cholera. Recent studies on the molecular nature of the virulence components that contribute to V. cholerae pathogenesis have provided insights into possible approaches for the development of a defined subunit cholera vaccine. Genetic analysis has demonstrated that the toxin-coregulated pilus (TCP) is the major factor that contributes to colonization of the human intestine by V. cholerae. In …
Dextran-Methylprednisolone Succinate As A Prodrug Of Methylprednisolone: Plasma And Tissue Disposition, Xiaoping Zhang, Reza Mehvar
Dextran-Methylprednisolone Succinate As A Prodrug Of Methylprednisolone: Plasma And Tissue Disposition, Xiaoping Zhang, Reza Mehvar
Pharmacy Faculty Articles and Research
Plasma and tissue disposition of a macromolecular prodrug of methylprednisolone (MP), dextran (70 kDa)–methylprednisolone succinate (DMP), was studied in rats. Single 5‐mg/kg doses of DMP or unconjugated MP were administered into the tail veins of different groups of rats (n = 4/group/time point). Blood (cardiac puncture) and tissues (liver, spleen, kidney, heart, lung, thymus, and brain) were collected at various times after DMP (0–96 h) or MP (0–2 h) injections. Concentrations of DMP and MP in samples were analyzed by size‐exclusion chromatography (SEC) and reversed‐phase high‐performance liquid chromatography (HPLC), respectively. Conjugation of MP with 70‐kDa dextran resulted in 22‐, …
Il-1 Induces Collagenase-3 (Mmp-13) Promoter Activity In Stably Transfected Chondrocytic Cells: Requirement For Runx-2 And Activation By P38 Mapk And Jnk Pathways, John A. Mengshol, Matthew P. Vincenti, Constance E. Brinckerhoff
Il-1 Induces Collagenase-3 (Mmp-13) Promoter Activity In Stably Transfected Chondrocytic Cells: Requirement For Runx-2 And Activation By P38 Mapk And Jnk Pathways, John A. Mengshol, Matthew P. Vincenti, Constance E. Brinckerhoff
Dartmouth Scholarship
Osteoarthritic chondrocytes secrete matrix metalloproteinase-13 (MMP-13) in response to interleukin-1 (IL-1), causing digestion of type II collagen in cartilage. Using chondrocytic cells, we previously determined that IL-1 induced a strong MMP-13 transcriptional response that requires p38 MAPK, JNK and the transcription factor NF-κB. Now, we have studied the tissue-specific transcriptional regulation of MMP-13. Constitutive expression of the transcription factor Runx-2 correlated with the ability of a cell type to express MMP-13 and was required for IL-1 induction; moreover, Runx-2 enhanced IL-1 induction of MMP-13 transcription by synergizing with the p38 MAPK signaling pathway. Transiently transfected MMP-13 promoters were not IL-1 …
Treatment Of Diabetes, John H.L. Playfair, Khalid M. Elased, Joseph B. De Souza
Treatment Of Diabetes, John H.L. Playfair, Khalid M. Elased, Joseph B. De Souza
Pharmacology and Toxicology Faculty Publications
The present invention provides methods for using a killed malaria parasite or an extract thereof in the treatment of non-insulin dependent diabetes mellitus (NIDDM).
Antioxidation Activity Of Dhea And Its Mechanisms, Sun Yang, Han Rui
Antioxidation Activity Of Dhea And Its Mechanisms, Sun Yang, Han Rui
Pharmacy Faculty Articles and Research
Objective: The aim of this study was to determine the anti-oxidative activity of a new chemopreventive agent--dehydroepiandrosterone (DHEA), and the mechanisms of action by which DHEA protect the thymocytes and DNA from oxidative damage. Methods: Agarose gel electrophoresis, flow cytometry, single cel! gel electrophoresis, chemiluminescence assay, triazolyl blue tetrazolumbromide (MTT) colorimetry, and three dimensional collagen gel assay were used. Results: In agarose gel electrophoresis, 10 nmol/L DHEA blocked the typical DNA degradation (DNA Ladder) induced by H2O2. DHEA 2. 5 nmol/L and 10 nmol/L both significantly decreased the percentage of characteristic apoptotic DNA …
Receptor Number And Caveolar Co-Localization Determine Receptor Coupling Efficiency To Adenylyl Cyclase, Rennolds S. Ostrom, Caroline Gregorian, Ryan M. Drenan, Yang Xiang, John W. Regan, Paul A. Insel
Receptor Number And Caveolar Co-Localization Determine Receptor Coupling Efficiency To Adenylyl Cyclase, Rennolds S. Ostrom, Caroline Gregorian, Ryan M. Drenan, Yang Xiang, John W. Regan, Paul A. Insel
Pharmacy Faculty Articles and Research
Recent evidence suggests that many signaling molecules localize in microdomains of the plasma membrane, particularly caveolae. In this study, overexpression of adenylyl cyclase was used as a functional probe of G protein-coupled receptor (GPCR) compartmentation. We found that three endogenous receptors in neonatal rat cardiomyocytes couple with different levels of efficiency to the activation of adenylyl cyclase type 6 (AC6), which localizes to caveolin-rich membrane fractions. Overexpression of AC6 enhanced the maximal cAMP response to β1-adrenergic receptor (β1AR)-selective activation 3.7-fold, to β2AR-selective activation only 1.6-fold and to prostaglandin E2 (PGE2) not at all. Therefore, the rank order of efficacy in …