Medicine and Health Sciences Commons^™

The Fate Of Sulfamethazine In Sodium-Hypochlorite-Treated Drinking Water: Monitoring By Lc-Msn-It-Tof, Tyler C. Melton, Stacy D. Brown

ETSU Faculty Works

Pharmaceutical compounds represent a rapidly emerging class of environmental contaminants. Such compounds were recently classified by the U.S. Geological Survey, including several antibiotics. An LC-MS/MS screening method for the top five antibiotics in drinking water was developed and validated using a Shimadzu LC-MS-IT-TOF. The separation was performed using a Waters Acquity UPLC BEH C18 column with a gradient elution. Sulfamethazine was exposed to conditions intended to mimic drinking water chlorination, and samples were collected and quenched with excess sodium sulfite. Kinetics of sulfamethazine degradation was followed as well as the formation of the major chlorinated byproduct (m/z 313). For the …