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Full-Text Articles in Medicine and Health Sciences
Glioma Stem Cells-Derived Exosomes Promote The Angiogenic Ability Of Endothelial Cells Through Mir-21/Vegf Signal, Xu Sun, Xiaotang Ma, Jinju Wang, Yuhui Zhao, Yue Wang, Ji C. Bihl, Yanfang Chen, Chuanlu Jiang
Glioma Stem Cells-Derived Exosomes Promote The Angiogenic Ability Of Endothelial Cells Through Mir-21/Vegf Signal, Xu Sun, Xiaotang Ma, Jinju Wang, Yuhui Zhao, Yue Wang, Ji C. Bihl, Yanfang Chen, Chuanlu Jiang
Pharmacology and Toxicology Faculty Publications
Glioma stem cells (GSCs) play an important role in glioblastoma prognosis. Exosomes (EXs) mediate cell communication by delivering microRNAs (miRs). Glioblastoma has a high level of miR-21 which could upregulate vascular endothelial growth factor (VEGF) expression. We hypothesized GSC-EXs can promote the angiogenic ability of endothelial cells (ECs) through miR-21/VEGF signal. GSCs were isolated from U-251 cells with stem cell marker CD133. GSCs transfected without or with scramble or miR-21 mimics were used to produce GSC-EXscon, GSC-EXssc and GSC-EXsmiR-21. Human brain ECs were co-cultured with vehicle, GSC-EXscon, GSC-EXssc or GSC-EXsmiR-21 plus …
Endothelial Progenitor Cells And Neural Progenitor Cells Synergistically Protect Cerebral Endothelial Cells From Hypoxia/Reoxygenation-Induced Injury Via Activating The Pi3k/Akt Pathway, Jinju Wang, Yusen Chen, Yi Yang, Xiang Xiao, Shuzhen Chen, Cheng Zhang, Bradley S. Jacobs, Bin Zhao, Ji C. Bihl, Yanfang Chen
Endothelial Progenitor Cells And Neural Progenitor Cells Synergistically Protect Cerebral Endothelial Cells From Hypoxia/Reoxygenation-Induced Injury Via Activating The Pi3k/Akt Pathway, Jinju Wang, Yusen Chen, Yi Yang, Xiang Xiao, Shuzhen Chen, Cheng Zhang, Bradley S. Jacobs, Bin Zhao, Ji C. Bihl, Yanfang Chen
Pharmacology and Toxicology Faculty Publications
Background
Protection of cerebral endothelial cells (ECs) from hypoxia/reoxygenation (H/R)-induced injury is an important strategy for treating ischemic stroke. In this study, we investigated whether co-culture with endothelial progenitor cells (EPCs) and neural progenitor cells (NPCs) synergistically protects cerebral ECs against H/R injury and the underlying mechanism.
Results
EPCs and NPCs were respectively generated from inducible pluripotent stem cells. Human brain ECs were used to produce an in vitro H/R-injury model. Data showed: 1) Co-culture with EPCs and NPCs synergistically inhibited H/R-induced reactive oxygen species (ROS) over-production, apoptosis, and improved the angiogenic and barrier functions (tube formation and permeability) in …