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Full-Text Articles in Medicine and Health Sciences
Fluorescence-Based Techniques For The Identification Of Small Biomolecules, Charnette Frederic
Fluorescence-Based Techniques For The Identification Of Small Biomolecules, Charnette Frederic
Seton Hall University Dissertations and Theses (ETDs)
Understanding fluorescence-based methods for detecting small biomolecules is a crucial area of focus in research investigations. Our studies focused on the challenge in two parts: Non-intrinsic fluorescence and intrinsic fluorescence. For studies on Non-intrinsic fluorescence, Rifampicin (Rif), an antibiotic harnessed for treating diverse bacterial infections, including tuberculosis (TB), serves as a focal point. Leveraging the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process, aptamers emerged from the nonspecific 40-mer Deoxyribonucleic Acid (DNA) library to interact with its target, Rif. During each SELEX cycle, the recovered nucleotides underwent amplification via Polymerase Chain Reaction (PCR) until the most selective aptamers were …
Exploring The Design Space Of Antifungal Peptides, Miryam Kikhwa
Exploring The Design Space Of Antifungal Peptides, Miryam Kikhwa
Seton Hall University Dissertations and Theses (ETDs)
The synthesis, characterization, and evaluation of antifungal activity of peptides FQ15 and FG10 will be conducted to address the hypothesis. The hypothesis of this thesis is that a peptide with similar properties to AS15 would have similar activity against Cryptococcus neoformans. This study addresses the challenge of drug- resistant microbes by developing inhibitors targeting lipid flippase and exploring their potential as antimicrobial agents against Cryptococcus neoformans. Chapter 1 provides a historical context of the background on the development of antimicrobials and common antifungal treatments, as well as an introduction about C. neoformans and its resistance mechanism. Chapter 2 introduces the …