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Impact Of Intracellular Glutathione Depletion On Neutral Sphingomyelinase In Human Hepatoma Hepg2 Cells, Marie Tanios
Impact Of Intracellular Glutathione Depletion On Neutral Sphingomyelinase In Human Hepatoma Hepg2 Cells, Marie Tanios
Theses and Dissertations
Introduction: Incidence of hepatocellular carcinoma (HCC) has been increasing dramaticallyandliver cancer is the fifth most common cancer globally. Increasing sphingomyelin (SM) hydrolysis via activation of the cell surface membrane-associated neutral sphingomyelinase (nSMase) has been linked to cell proliferation and apoptosis. Glutathione (GSH) depletion was reported to mediate induction of apoptosis via nSMase activation. On the other hand, other studies reported that GSH protected cancer cells against apoptosis. We hypothesized that GSH synthesis inhibition with buthioninesulfoximine (BSO) increases HepG2 cell proliferation. Objectives: The aim of the current study wasto investigate the effect of GSH depletion using BSO on HepG2 cell proliferation …