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Microscopic Matrix Remodeling Precedes Endothelial Morphological Changes During Capillary Morphogenesis, Claire Mcleod, John Higgins, Yekaterina Miroshnikova, Rachel Liu, Aliesha Garrett, Alisha Sarang-Sieminski

Alisha L. Sarang-Sieminski

The formation of microvascular networks (MVNs) is influenced by many aspects of the microenvironment, including soluble and insoluble biochemical factors and the biophysical properties of the surrounding matrix. It has also become clear that a dynamic and reciprocal interaction between the matrix and cells influences cell behavior. In particular, local matrix remodeling may play a role in driving cellular behaviors, such as MVN formation. In order to explore the role of matrix remodeling, an in vitro model of MVN formation involving suspending human umbilical vein endothelial cells within collagen hydrogels was used. The resulting cell and matrix morphology were microscopically …