Open Access. Powered by Scholars. Published by Universities.®
- Publication
- File Type
Articles 1 - 4 of 4
Full-Text Articles in Medicine and Health Sciences
Nonenzymatic Glycosylation Of Erythrocyte Membrane Proteins. Relevance To Diabetes, J A. Miller, Ellen M. Gravallese, H F. Bunn
Nonenzymatic Glycosylation Of Erythrocyte Membrane Proteins. Relevance To Diabetes, J A. Miller, Ellen M. Gravallese, H F. Bunn
Ellen M. Gravallese
Nonenzymatic glycosylation of proteins of the erythrocyte membrane was determined by incubating erythrocyte ghosts with [3H]borohydride. The incorporation of tritium into protein provides a reliable assay of ketoamine linkages. The membrane proteins from 18 patients with diabetes incorporated twice as much radioactivity as membrane proteins from normal erythrocytes. After acid hydrolysis, amino acid analysis showed that the majority of radioactivity was localized to glucosyllysine. Autoradiograms showed that all of the major proteins of the erythrocyte membrane, separated by electrophoresis on sodium dodecyl sulfate gels, contained ketoamine linkages. No protein bands in either normal or diabetic erythrocytes showed significant preferential labeling. …
Diabetic Rates And Mice Are Resistent To Porcine And Human Insulin: Flawed Experimental Models For Testing Islet Xenograft, A. Pepper, C. Gall, D. Mazzuca, C.W.J. Melling, D. White
Diabetic Rates And Mice Are Resistent To Porcine And Human Insulin: Flawed Experimental Models For Testing Islet Xenograft, A. Pepper, C. Gall, D. Mazzuca, C.W.J. Melling, D. White
Jamie Melling
BACKGROUND: Islet transplantation is potentially a promising therapy for the restoration of carbohydrate control to diabetic patients. However, the global application of islet transplantation requires a ubiquitous source of beta cells. The xenotransplantation of porcine islets would provide such a source. Success in porcine islet xenografting has been achieved in diabetic primates. However, there are few reports of reversal of diabetes with porcine islet xenografts in rodent models of diabetes, relative to the number of successful rodent experiments performed as allografts. Here we report for the first time the inability of porcine (and human) insulin to control blood glucose levels …
Imaging Of Transplanted Pancreatic Islets In Vivo, P. Joo Ho Tai, C. Foster, C. Hasil, C.W.J. Melling, D. White
Imaging Of Transplanted Pancreatic Islets In Vivo, P. Joo Ho Tai, C. Foster, C. Hasil, C.W.J. Melling, D. White
Jamie Melling
No abstract provided.
Imaging Islets Labeled Wth Magnetic Nanoparticules At 1.5 Tesla, J.H. Tai, Paula Foster, Alma Rosales, Biao Feng, Craig Ha, Violetta Martinez, Soha Ramadan, Jonatan Snir, C.W. Melling, Savita Dhanvantari, Brian Rutt, David White
Imaging Islets Labeled Wth Magnetic Nanoparticules At 1.5 Tesla, J.H. Tai, Paula Foster, Alma Rosales, Biao Feng, Craig Ha, Violetta Martinez, Soha Ramadan, Jonatan Snir, C.W. Melling, Savita Dhanvantari, Brian Rutt, David White
Jamie Melling
We have developed a magnetic resonance imaging (MRI) technique for imaging Feridex (superparamagnetic iron oxide [SPIO])-labeled islets of Langerhans using a standard clinical 1.5-Tesla (T) scanner and employing steady-state acquisition imaging sequence (3DFIESTA). Both porcine and rat islets were labeled with SPIO by a transfection technique using a combination of poly-l-lysine and electroporation. Electron microscopy demonstrated presence of SPIO particles within the individual islet cells, including beta-cells and particles trapped between cell membranes. Our labeling method produced a transfection rate of 860 pg to 3.4 ng iron per islet, dependent on the size of the islet. The labeling procedure did …