Medicine and Health Sciences Commons^™

Store-Operated Calcium Channels In The Function Of Intracardiac Neurons, Timetria Bonds

USF Tampa Graduate Theses and Dissertations

Proper autonomic regulation of mammalian cardiac function is dependent upon very complex and precise communication among the intracardiac ganglia and individual neurons within the ganglia. An array of neuromodulators is found within the ganglia that direct neuronal activity by modulating the movement of calcium. The current study determines that opioidergic agonists, which have been found to contribute to severe cardiac disease states and intracellular calcium mobilization, are also responsible for changes in the function of the intracardiac neuron via their effects on store-operated calcium channels (SOCs).

Previous studies suggest that phosphorylation plays a role in SOC regulation. Using Fura-2 calcium …

An Examination Of Diet, Acculturation And Risk Factors For Heart Disease Among Jamaican Immigrants, Carol Renee Oladele

USF Tampa Graduate Theses and Dissertations

Background: The South Florida region is home to over 85,000 Jamaican immigrants. Yet, little is known about the dietary intakes and predictors of risk of disease within this immigrant group. An assessment of dietary intakes and the development of dietary intake methodologies specific to the Jamaican population was important as it permitted accurate estimation of the nutrient intakes of this immigrant population whose dietary habits are not well documented. In addition, nothing is known about the prevalence of risk factors for heart disease or factors influencing risk factors among this immigrant group. The purpose of this study was to assess …

"Not If, But When": Sex, Risk, And Trust In Timing Gardasil Vaccine Decisions, An Exploratory Study Among Healthcare Providers And Middle-Class Parents In The U.S., Kathleen Marie Brelsford

USF Tampa Graduate Theses and Dissertations

This dissertation research explores how values regarding sexuality, morality, responsibility, protection, trust, and risk — expressed through parent, daughter, and healthcare provider relationships and interactions — inform parental decisions regarding the Gardasil® vaccine. In particular, the research examines the competing and conflicting meanings that parents and providers ascribe to vaccination and how actors position the vaccine within a wider set of negotiated, value–laden discourses. Because these narratives are situated within a larger structural field that shapes the landscape in which providers and parents interact, relevant historical and structural factors, including vaccine policy, cost, and compensation are discussed.

Prophylactic, Risk-Reducing Surgery In Unaffected Brca-Positive Women: Quality Of Life, Sexual Functioning And Psychological Well-Being, Sharon Tollin

USF Tampa Graduate Theses and Dissertations

Women with an inherited BRCA mutation are at significantly increased risk for breast and ovarian cancer, often diagnosed at an earlier age than sporadic cancers. Prophylactic surgery, with bilateral mastectomy and/or bilateral prophylactic salpingo-oophorectomy, represents an option for risk reduction. The purpose of this study was to explore quality of life, sexual functioning, menopausal symptoms, psychological well-being and satisfaction with risk management decisions for BRCA-positive women ages 21 to 50 (M = 38.4 years), without a personal history of cancer. A web-based, cross-sectional study design was utilized to compare women opting for any prophylactic surgery (n = 160) with those …

Dengue Fever In Tegucigalpa, Honduras: Use Of The Explanatory Model In A Sample Of Urban Neighborhoods To Contextualize And Define Dengue Fever Among Community Participants, Jose Enrique Hasemann

USF Tampa Graduate Theses and Dissertations

This project elucidated the explanatory model of dengue fever held by members of urban communities in Tegucigalpa, Honduras. The study was conducted over a four-month period from May-August of 2011, and it was divided into two stages. The first stage of the project consisted of volunteer participation with dengue fever surveillance brigades in the three communities with the highest incidence of dengue fever during the beginning of 2011. This initial stage employed participant observation as its research method. The second stage was conducted in a different community within Tegucigalpa. The primary research methods employed during the second stage of the …

Accuracy Of Educator Nominations In Identifying Students With Elevated Levels Of Anxiety And Depression, Jennifer Cunningham

USF Tampa Graduate Theses and Dissertations

Internalizing disorders, specifically depression and anxiety, affect up to 18% and 33% of youth, respectively (Costello, Egger, & Angold, 2005b). Schools have become a major provider of mental health services to children, primarily in attempts to overcome barriers to receiving community services (Farmer, Burns, Philip, Angold, & Costello, 2003). As such, it is important that schools have effective mechanisms in place to accurately identify students who may be in need of such services. The current study examined the accuracy of one such method, educator nominations (including from both teachers and school-based mental health professionals) in identifying students who self-report elevated …

The Role Of Male Partners In Childbirth Decision Making: A Qualitative Exploration With First-Time Parenting Couples, Sharon Dejoy

USF Tampa Graduate Theses and Dissertations

Fathers' attendance at childbirth is almost universal in the United States, but few researchers have addressed the role that males play in childbirth decision making. The number of technological interventions available to birthing women is increasing, as is the utilization of those interventions. The degree to which women choose or agree to these interventions plays a major role in individual and societal health outcomes. Therefore, health care policy makers and educators must find ways to help childbearing couples navigate the complex maze of decisions related to childbirth. However, policies and programs to increase shared decision making may not function as …

The Holistic Complementary Structure Of Western Bio-Medicine And Traditional Healing And Achieving Complete Health, Candace Gail Oubre

USF Tampa Graduate Theses and Dissertations

Achieving complete health requires a deep understanding of complementary cultural competency sensitivity between physician and patient. This may include but is not limited to access to preventative health care resources, access to health educational resources and access to cultural healing resources, for example, shamans, Ayurvedic physicians, and herbal healers. Advocates of cultural competency emphasize great importance on knowledge of the patients' cultural background; however, the transcendence of this knowledge can be explained further through complementary cultural competency sensitivity. This is when the cultures of the physician and patient complement each other in terms of understanding what is in the patients' …

Emotional Reactivity And Regulation In Current And Remitted Depression: An Event Related Potential Study, Lauren M. Bylsma

USF Tampa Graduate Theses and Dissertations

Major Depressive Disorder (MDD) is thought to be characterized by emotion regulation deficits, including decreased use of adaptive strategies such as reappraisal, but little is known about the exact nature of these deficits and whether or not they are specific to the depressed mood state. The late positive potential (LPP) is a sustained positive deflection of the event-related potential (ERP) associated with responding to emotionally-valenced stimuli, and reappraisal strategies have been found to reduce LPP magnitude in response to emotional stimuli in healthy individuals, but this effect has not been examined in MDD. This study utilized ERPs to examine emotional …

Characterization Of The Serologic Responses To Plasmodium Vivax Dbpii Variants Among Inhabitants Of Pursat Province, Cambodia, Samantha Jones Barnes

USF Tampa Graduate Theses and Dissertations

The Plasmodium vivax Duffy Binding Protein (DBP) is the ligand in the major pathway for P. vivax invasion of human reticulocytes, making it an appealing vaccine candidate. Region II of DBP (DBP-RII) is the minimal portion of the ligand that mediates recognition of the Duffy Antigen Receptor for Chemokines (DARC receptor) on the reticulocyte surface and constitutes the primary vaccine target. Analysis of natural variation in the coding sequences of DBP-RII revealed signature evidence for selective pressure driving variation in the residues of the putative receptor-binding site. We hypothesize that anti-DBP immunity in P. vivax infections is strain-specific and hindered …

Discovery Of A Functional Ecdysone Response Element In Brugia Malayi, Tracy Enright

USF Tampa Graduate Theses and Dissertations

The aim of this study was to determine whether functional ecdysone response elements (EcREs) exist within the genome of Brugia malayi, a parasitic nematode that causes lymphatic filariasis. The hypothesis that EcREs exist in B. malayi stemmed from previous demonstration of a functional ecdysone response system in B. malayi (Tzertzinis et al., 2010). Real-time PCR (qPCR) experiments were conducted to measure gene expression levels for twelve genes proximal to five putative EcREs in 20-OH ecdysone treated and untreated B. malayi embryos. Seven genes showed consistent upregulation with 20-OH ecdysone treatment. Each of the five putative EcREs had at least one …

An Observation Of Immunological Effect, A Diet Enhanced With Spirulina And Treatment With Fractalkine In Models Of Parkinson's Disease, Mibel M. Pabón

USF Tampa Graduate Theses and Dissertations

In my dissertation research we used use human wild type α-synuclein gene expression using an adeno-associated viral vector (AAV9) that induced a slowly progressive loss of dopamine (DA) neurons in the Substantia nigra (SN) as one of our animal model of Parkinson’s disease (PD). It is our hypothesis that neuroinflammation predisposes the brain to susceptibility to neurodegenerative diseases. Thus we examined the progression of a PD lesion and examined the manipulations of the immune system to understand further the inflammatory role when we administered exogenous soluble fractalkine.

The specific etiology of neurodegeneration in PD is unknown, but the inflammatory mechanisms …

Evaluation Of Preference For Exergames Among Elementary Students, Christie Ann Cacioppo

USF Tampa Graduate Theses and Dissertations

Obesity in children is a nationwide problem. Physical activity is one way to help children stay fit and prevent obesity. Unfortunately, access to technology involving sedentary behavior is easier than ever. Fortunately, for this generation of students there is a way to combine physical activity and technology through exergaming. In order to encourage students to participate in exergaming, the students should be able to play games they prefer. The purpose of this study was to apply a paired choice preference assessment, rank order card sort, and social validity surveys to determine the preference order of six exergames for six elementary …

An Evaluation Of Booster Training Using Video Modeling With Foster Parents, Anna Katherine Caravello

USF Tampa Graduate Theses and Dissertations

In order for foster parents to be prepared to manage problem behavior and develop a positive relationship with a child in their home, local foster care agencies require these parents to attend parent training classes. Unfortunately, even foster care agencies that offer empirically validated parent training courses are unable to prevent the parents' performance from decreasing over time (Cowart, Iwata, & Poynter, 1984; Forehand & King, 1977; Mueller et al., 2003). However, researchers have created booster training sessions to counteract this issue. Booster training sessions allow participants to attend a brief refresher course on skills they have previously learned. Another …

Physical Activity Promotion Among School-Aged Children Using Pedometers And Rewards, Kari E. Ek

USF Tampa Graduate Theses and Dissertations

Physical activity is important for children as many children are considered overweight or obese. The benefits of exercise have been demonstrated in empirical studies across all age ranges (Horne, Hardman, Lowe, & Rowlands, 2009; Kelly et al., 2004; Louie & Chan, 2003; Southard & Southard, 2006). In the current study, a multiple baseline design across participants was used to assess the effectiveness of goal setting, reinforcement contingencies, and pedometers that provide feedback to increase step count of 5 participants. During baseline each participant wore a sealed pedometer to assess the average steps the participants took per day. After baseline, each …

Contextualizing Hiv/Aids Prevention And Treatment Programs In Zanzibar, Tanzania, Naheed Ahmed

USF Tampa Graduate Theses and Dissertations

International aid organizations and wealthy nations have contributed billions to combat the spread and treatment of HIV/AIDS in sub-Saharan Africa; however, these programs have been critiqued for not addressing the socioeconomic and cultural context of the epidemic, instead relying upon generalized approaches. The prevalence rate in Zanzibar, Tanzania is low in the general population, but high among vulnerable segments of Zanzibari society, resulting in interventions focusing on particular groups (e.g. sex workers, drug users, and men who have sex with men). Through interviews with government agencies, non-profit organizations, medical professionals, vulnerable populations, and HIV/AIDS patients, this paper examines how local …

Validation Of The Usf Safe Exposure Time Equation For Heat Stress, Arden Bruce Andersen

USF Tampa Graduate Theses and Dissertations

Heat stress conditions are prevalent in the working environment around the world. Often they are not readily engineered out. Administrative controls and, in extreme/toxic environments, personal protective gear are the means available to protect workers. For every combination of metabolic work rate, clothing ensemble and environmental WBGT, there is a time of exposure threshold, beyond which the worker can no longer compensate for the heat stress, and signs and symptoms of heat strain appear. Increasingly, worker environments require specialty clothing either for worker protection or to maintain a clean/sanitary environment. Prior to the publication of the USF safe exposure time …

The Natural History Of Human Papillomavirus Related Condyloma In A Multinational Cohort Of Men, Gabriella Anic

USF Tampa Graduate Theses and Dissertations

Human papillomavirus (HPV) is the most common sexually transmitted infection in the United States, but few studies have examined the progression from HPV infection to disease in men. Genital condyloma are the most common clinical manifestation of HPV infection. Though not associated with mortality, condyloma are a source of emotional distress, and treatment is often painful with a high recurrence rate. The aims of this study were to examine the distribution of HPV types present on the surface of condyloma, estimate the incidence of condyloma overall and after type-specific HPV infections, assess the sociodemographic and sexual behavior factors independently associated …

Identification And Characterization Of The Human Herpesviruses 6a And 6b Genome Integration Into Telomeres Of Human Chromosomes During Latency, Jesse Herbert Arbuckle

USF Tampa Graduate Theses and Dissertations

While the latent genome of most Herpesviruses persists as a nuclear circular episome, previous research has suggested that Human Herpesvirus 6 (HHV-6) may integrate into host cell chromosomes, and be vertically transmitted in the germ-line. Because the HHV-6 genome encodes a perfect TTAGGG telomere repeat array at the right end direct repeat (DR_R) and an imperfect TTAGGG repeat at the end of the left end direct repeat (DR_L), we established a hypothesis that during latency, the HHV-6A and HHV-6B genome integrates into the telomeres of human chromosomes through homologous recombination with the n(TTAGGG) viral repeats, and …

Drug Courts Work, But How? Preliminary Development Of A Measure To Assess Drug Court Structure And Processes, Blake Barrett

USF Tampa Graduate Theses and Dissertations

The high prevalence of substance use disorders is well-documented among criminal offenders. Drug courts are specialty judicial programs designed to: 1) improve public safety outcomes; 2) reduce criminal recidivism and substance abuse among offenders with substance use disorders; and 3) better utilize scarce criminal justice and treatment resources. Drug courts operate through partnerships between the criminal justice, behavioral health and public health systems. Offenders participate in an intensive regimen of substance abuse treatment and case management while under close judicial supervision. Drug courts' effectiveness in reducing criminal recidivism and drug use has been documented through numerous primary studies as well …

Differential Effects Of Isoflurane And Propofol Anesthesia On Neurogenesis In Young And Aged Rats, Diana Marcela Erasso

USF Tampa Graduate Theses and Dissertations

Worldwide, millions of young and elderly patients receive procedures that could not be performed without the use of anesthetics. Unfortunately, emerging animal and human data suggest an association between exposure to general anesthesia and impairment of cognitive function in pediatric and geriatric patients. Recent laboratory data have shown that general anesthetics are potentially damaging to the developing and aging brain. However, the mechanism by which this happens is still unknown. General anesthetics affect learning and memory, a brain function involving neural plasticity. An important form of neural plasticity receiving attention is postnatal neurogenesis. This process is highly regulated and involved …

Navigating The Child Welfare System: An Exploratory Study Of Families' Experiences, Lianne Fuino Estefan

USF Tampa Graduate Theses and Dissertations

Growing up in a family environment that includes child maltreatment can result in an array of negative consequences for children, including health, behavioral, developmental, and social difficulties, and these consequences can persist over the lifetime. Families who have come to the attention of child welfare services for child maltreatment are at particularly high risk for experiencing multiple concurrent problems, including intimate partner violence, substance abuse, and mental health issues, as well as other family challenges. It is essential to intervene effectively with this population. However, there are few qualitative studies of parent experiences in the child welfare system through which …

Actualizing Empowerment: Developing A Framework For Partnering With Families In System Level Service Planning And Delivery, Kathleen Ferreira

USF Tampa Graduate Theses and Dissertations

The engagement of families of children and adolescents with Serious Emotional Disturbance (SED) as full partners in individual treatment, organizational, and system level decision making has become an important focus for systems of care (SOCs) serving youth with emotional and behavioral challenges. SOCs typically include cross-agency partnerships with mental health, child welfare, juvenile justice, and education for the purpose of providing services and supports for youth with SED who have multi-agency needs. Implementation of a federal mandate requiring family driven care (FDC) within systems of care funded through the Children's Mental Health Initiative (CMHI) has revealed that most system of …

A Pilot Study Of Small-Scale Variations In Outdoor Benzene Concentrations, Samantha Catherine Fridh

USF Tampa Graduate Theses and Dissertations

Benzene is an important toxic chemical in urban air and known human carcinogen released substantially by mobile sources. It's important to understand the spatial variation of benzene concentrations in order to understand exposures of susceptible sub-populations such as children and minority groups. Current monitoring networks use large and expensive air samplers that require electricity and restrict the location and number of samplers, not allowing for fine spatial resolution data.

The goals of this study are to develop and evaluate protocols for passive sampling and analysis of ambient benzene concentrations, and conduct a pilot study investigating small-scale variations over an area …

A Meta-Analysis Of Cultural Competence Education In Professional Nurses And Nursing Students, Ruth Wilmer Gallagher

USF Tampa Graduate Theses and Dissertations

Cultural competence learning interventions have been suggested to positively improve knowledge, attitudes, and behaviors in both professional nurses and nursing students. A meta-analysis was used to examine the effectiveness of learning interventions designed to increase the cultural competence in professional nurses and nursing students. This is the first known meta-analysis of studies on cultural competence learning interventions in professional nurses and nursing students.

The meta-analysis was done using 13 research studies on cultural competence educational interventions from 1999 to 2010 that were published peer-reviewed literature found in electronic databases. Analyses were computed using a fixed-effect model and effect size data …

The Role Of Hiv-1 Proteins In Alzheimer's Disease Pathology, Brian Nelson Giunta

USF Tampa Graduate Theses and Dissertations

Prevalence of HIV-associated cognitive impairment is rising, the worst form of which is HIV-associated dementia (HAD). The disease is fuiled by a chronic innate type pro-inflammatory response in the brain which is highly dependent upon the activation of microglia. We first created an in vitro model of HAD composed of cultured microglial cells synergistically activated by the addition of IFN-gamma and the HIV-1 coat glycoprotein, gp120. This activation, as measured by TNF-alpha and NO release, is synergistically attenuated through the alpha7nAChR and p44/42 MAPK system by pretreatment with nicotine, and the cholinesterase inhibitor, galantamine. As these medications have been FDA …

The Impact Of Managed Care On The Utilization And Distribution Of Inpatient Surgical Procedures With Demonstrated Volume And Outcome Endogeneity, Linda Stephens Gipson

USF Tampa Graduate Theses and Dissertations

Purpose

This study is designed to determine whether managed care has had an influence on the number and distribution of procedures with demonstrated volume and outcome endogeneity in Florida healthcare markets; in addition, methods are developed to determine which measures of managed care activity best predict the impact of managed care in health care markets.

Rationale

A retrospective population based cohort design is used capitalizing on the variability among Florida markets between 1995 and 1999, a period which captured the full business life cycle of managed care plans statistical areas and competing hospitals (market share) over time. Multiple regression models …

Apparent Total Evaporative Resistance Values From Human Trials Over A Range Of Heat Stress Levels, Brian Grace

USF Tampa Graduate Theses and Dissertations

Clothing can influence heat stress depending on the design and its ability to act as a barrier. The progressive heat stress protocol permitted the collection of data to empirically estimate the apparent total evaporative resistance (R_e,T,a). Five different clothing ensembles were evaluated, which included work clothes, cotton coveralls, and three limited-use protective clothing ensembles including a pthesis-barrier ensemble, (Tyvek^® 1424), water-barrier, vapor-permeable ensemble (NexGen^® LS 417), and a vapor-barrier ensemble (Tychem QC^®). The study design called for three metabolic level's: low, moderate, and high (L, M, & H) and three heat stages: compensable, transitional, …

The Role Of Lipoproteins/Cholesterol In Genomic Instability And Chromosome Mis-Segregation In Alzheimer's And Cardiovascular Disease, Antoneta Granic

USF Tampa Graduate Theses and Dissertations

Several lines of evidence link Alzheimer's disease (AD) to atherosclerosis (CVD), including that elevated low density lipoprotein (LDL)-cholesterol is a common risk factor. Development of genomic instability could also link the two diseases. Previous fluorescence in situ hybridization (FISH) analyses revealed a clonal expansion of aneuploid smooth muscle cells underlying atherosclerotic plaques. Likewise, cellular and mouse models of AD revealed tau-dependent mitotic defects and subsequent aneuploidy partly resulting from amyloid-beta (A&beta) interference with microtubule (MT) stability, and specific MT motors function. Moreover, AD patients develop aneuploid/hyperploid cells in brain and peripheral tissues, implicating similar mechanism that may lead to apoptosis …

The Mediating Role Of Social Support And Fulfillment Of Spiritual Needs In End Of Life Care, Kimberley A. Gryglewicz

USF Tampa Graduate Theses and Dissertations

End of life (EOL) caregiving can be a daunting and challenging endeavor as caregivers adjust to the ever-changing care demands associated with dying. Increased personal care, assisting with symptom and medication management, and attending to the emotional and spiritual needs of the dying person require caregivers to learn new tasks and to assume new roles such as social worker, nurse, and chaplain. As families continue to play an essential role in meeting the health care needs of their dying loved ones, it is imperative for social workers to understand the complexities of the end of life caregiving experience in order …