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Articles 1 - 25 of 25
Full-Text Articles in Medicine and Health Sciences
An Ecological Perspective Of American Rodent-Borne Orthohantavirus Surveillance, Nathaniel Mull
An Ecological Perspective Of American Rodent-Borne Orthohantavirus Surveillance, Nathaniel Mull
Graduate Theses and Dissertations
Orthohantaviruses are a global group of viruses found primarily in rodents, though several viruses have also been found in shrews and moles. Many rodent-borne orthohantaviruses are capable of causing one of several diseases in humans, and the mortality associated with these diseases ranges from < 0.1% - 50% depending on the specific etiological virus. In North and South America, orthohantavirus research was ignited by an outbreak of severe disease in the Four Corners region of the United States in 1993. However, despite the discovery of over 20 orthohantaviruses in the Americas, our understanding of orthohantavirus ecology and virus-host dynamics in this region is still limited, and orthohantavirus surveillance is generally restricted in scope to select regions and small portions of host distributional ranges. In Chapter I, I present a literature review on the current understanding of American rodent-borne orthohantavirus ecology. This review focused on under-studied orthohantaviruses, addressing gaps in knowledge by extrapolating information from well-studied orthohantaviruses, general rodent ecology, and occassionally from Eurasian orthohantavirus-host ecology. There were several key conclusions generated from this review that warrant further research: 1) the large number of putative orthohantaviruses and gaps in orthohantavirus evolution necessitate further surveillance and characterization, 2) orthohantavirus traits differ and are more generalizable based on host taxonomy rather than geography, and 3) orthohantavirus host species are disproportionately found in grasslands and disturbed habitats. In Chapter II, I present a prioritized list of rodent species to target for orthohantavirus surveillance based on predictive modeling using machine learning. Probable orthohantavirus hosts were predicted based on traits of known orthohantavirus hosts using two different types of evidence: RT-PCR and virus isolation. Predicted host distributions were also mapped to identify geographic hotspots to spatially guide future surveillance efforts. In Chapter III, I present a framework for understanding and predicting orthohantavirus traits based on reservoir host phylogeny, as opposed to the traditional geographic dichotomy used to group orthohantaviruses. This framework establishes three distinct orthohantavirus groups: murid-borne orthohantaviruses, arvicoline-borne orthohantaviruses, and non-arvicoline cricetid-borne orthohantaviruses, which differ in several key traits, including the human disease they cause, transmission routes, and virus-host fidelity. In Chapter IV, I compare rodent communities and orthohantavirus prevalence among grassland management regimes. Sites that were periodically burned had high rodent diversity and a high proportion of grassland species. However, rodent seroprevalence for orthohantavirus was also highest in burned sites, representing a trade-off in habitat management outcomes. The high seroprevalence in burned sites is likely due to the robust populations supported by the high quality habitat resulting from prescribed burning. In Chapters V and VI, I describe Ozark virus and Sager Creek virus, two novel orthohantaviruses discovered from specimens collected during Chapter IV. Both chapters report full genome sequences of the respective viruses and compare both nucleotide and protein phylogenies with related orthohantaviruses. Additionally in Chapter VI, I support the genetic analyses with molecular and ecological characterizations, including seasonal fluctuations in host abundance, correlates of prevalence, evidence of virus shedding, and information on host cell susceptibility to Sager Creek virus.
Intestinal Microbiota Analysis Of Broiler Chickens Under Necrotic Enteritis Challenge And Tributyrin Supplementation, Taylor Nicole Mckinney
Intestinal Microbiota Analysis Of Broiler Chickens Under Necrotic Enteritis Challenge And Tributyrin Supplementation, Taylor Nicole Mckinney
Graduate Theses and Dissertations
Poultry is a staple protein source for most of the planet. Until recently, antibiotic growth promoters (AGPs) were used to prevent illnesses in commercial chicken production. Currently, this is not possible due to regulations and consumer concern, but without such a preventative, diseases like necrotic enteritis (NE) have reemerged, posing a threat to bird health, and ultimately, our food source. Necrotic enteritis is a severe gastrointestinal disease caused by the gram-positive pathogen, Clostridium perfringens. Clinical features of this disease are diarrhea, intestinal lesions, and death, with a high transmission rate. In a subclinical form, growth performance is diminished and is …
Histomonosis In Turkeys – Factors Influencing Transmission, Pathogenesis, And The Search For Prophylactic Or Therapeutic Compounds, Thainá Landim De Barros
Histomonosis In Turkeys – Factors Influencing Transmission, Pathogenesis, And The Search For Prophylactic Or Therapeutic Compounds, Thainá Landim De Barros
Graduate Theses and Dissertations
The incidence of histomonosis has been increasing in poultry since the ban of prophylactic and therapeutic compounds. Histomonosis is caused by the protozoa Histomonas meleagridis. The objective of this dissertation was to investigate factors impacting the pathogenesis and transmission of histomonosis and to evaluate compounds that could potentially prevent or reduce the severity of histomonosis in turkeys. In the first study, the effect of sodium chlorate and sodium nitrate on reducing histomonads growth was tested in vitro and added to a basal turkey diet. A decrease in the growth of histomonads in vitro was observed, but no in vivo effect …
Investigation Of Histomoniasis Prevention In Poultry, Lesleigh Beer
Investigation Of Histomoniasis Prevention In Poultry, Lesleigh Beer
Graduate Theses and Dissertations
Histomonas meleagridis is the etiological agent of histomoniasis, also commonly known as blackhead disease. This protozoal disease of poultry is detrimental to turkeys with flock mortalities often reaching 80-100%, although other gallinaceous birds are susceptible. Since the voluntary removal of nitarsone in 2015, the poultry industry is suffering with no approved prophylactics, therapeutics, or vaccines for this disease. The objectives of this dissertation were to evaluate multiple methods for prevention or control of histomoniasis, including dietary chemoprophylaxis and vaccination. Specifically, this research evaluated quinine as a chemoprophylactic candidate (Chapter 3) or live-attenuated H. meleagridis as vaccine candidates (Chapter 4) in …
Directed Genome Evolution To Identify Genes For Macrophage Survival By Staphylococcus Agnetis, Sonali Lakshika Anne Lenaduwe Lokuge
Directed Genome Evolution To Identify Genes For Macrophage Survival By Staphylococcus Agnetis, Sonali Lakshika Anne Lenaduwe Lokuge
Graduate Theses and Dissertations
Bacterial Chondronecrosis with Osteomyelitis (BCO) is a debilitating infection that negatively impacts animal welfare and costs the broiler industry billions of dollars annually. We have previously isolated Staphylococcus agnetis 908 from BCO samples obtained from broilers at the University of Arkansas research farm. This isolate can induce BCO lameness at greater than 50% in broilers exposed to the pathogen in drinking water. We found that S. agnetis 908 is capable of surviving and escaping macrophages compared to a closely related cattle isolate,1379. Through Directed Genome Evolution (DGE) we identified that this difference is at least partially associated with an alanine …
Examining Pathogenesis And Preventatives In Spontaneous And Staphylococcus-Induced Bacterial Chondronecrosis With Osteomyelitis In Broilers, Nnamdi Simon Ekesi
Examining Pathogenesis And Preventatives In Spontaneous And Staphylococcus-Induced Bacterial Chondronecrosis With Osteomyelitis In Broilers, Nnamdi Simon Ekesi
Graduate Theses and Dissertations
Bacterial chondronecrosis with osteomyelitis is the most important cause of lameness in broilers. This is important to poultry production, as it poses animal welfare issues, and causes a significant loss in revenue. The remediation of this disease requires the study of its etiology with fitting models and evaluating preventatives. The research reported herein covers genomic virulence analysis of BCO isolates, mainly Staphylococcus aureus, and Escherichia coli retrieved from lame birds. We found that S. aureus isolates were closest to chicken strains in Europe but may have been in the Arkansas area for a decade. Phylogenomics suggest our S. aureus is …
Biogeography Of The Bovine Respiratory Microbiome And Its Relationship With Bovine Respiratory Disease, Jianmin Chai
Biogeography Of The Bovine Respiratory Microbiome And Its Relationship With Bovine Respiratory Disease, Jianmin Chai
Graduate Theses and Dissertations
Bovine respiratory disease (BRD) is the most common and costly disease in the beef cattle industry, leading to high morbidity, mortality and huge economic loss. Despite the recent advances in vaccination and antimicrobial techniques, no significant health-improved outcomes have developed. Due to a deep investigation of the microbiome, respiratory microbiotas are known to have important roles for host health and disease. However, BRD specific pathogens have not yet been identified since they are found in both healthy and diseased animals. A systemic and comprehensive study of the biogeography of the bovine respiratory microbiome and its relationship with BRD is lacking …
Effect Of Feed Additives And Toxic Elements On Swine Growth Performance, Nutrient Digestibility, Immune Function And Reproductive Performance, Richard A. Mudarra Hernández
Effect Of Feed Additives And Toxic Elements On Swine Growth Performance, Nutrient Digestibility, Immune Function And Reproductive Performance, Richard A. Mudarra Hernández
Graduate Theses and Dissertations
To evaluate the effects of peptide in combination of zinc oxide (Zno) or acidifiers (Exp 1), and gossypol (G) from cottonseed meal (CSM) ( Exp 2&3) on growth performance, complete blood cell counts (Exp 1, 2&3), nutrient digestibility (Exp 1), plasma gossypol (Exp 2&3) and semen quality (Exp 3), weaned pigs (Exp 1), growing gilts (Exp 2) and growing boars (Exp 3) were randomly allotted to dietary treatments. Treatments for Exp 1 during phase 1&2 were: (1) Positive Control (PC), formulated to meet NRC (2012) nutrient requirements; (2) Negative control (NC), fish meal was reduced to achieve -0.13% SID lysine; …
Fate And Dissemination Of Salmonella Reading In Market-Age Turkeys At Processing Using Oral Gavage Challenge Model, Aaron Ashcraft
Fate And Dissemination Of Salmonella Reading In Market-Age Turkeys At Processing Using Oral Gavage Challenge Model, Aaron Ashcraft
Graduate Theses and Dissertations
This study aimed to evaluate the fate and dissemination of Salmonella Reading (SR) in market-age turkeys using an oral gavage challenge model. One hundred twenty-eight-week-old commercial turkey hens were moved from commercial production to research facilities. Upon arrival, a combination of enrofloxacin, 10 mg/kg, and florfenicol, 20 mg/kg, were orally administered sequentially before comingled placement on fresh pine shavings. Turkeys were challenged with 108 cfu SR by oral gavage on days 4 and 7 post-placement. Subsets were subjected to simulated commercial processing on days 14 (n=40), 21 (n=40) and 28 (n=32) post-placement (corresponding to 10, 11, and 12 weeks of …
Associations Among Beef Cattle Genotypes, Neospora Caninum Infection, And Reproductive Performance, Ryan James Page
Associations Among Beef Cattle Genotypes, Neospora Caninum Infection, And Reproductive Performance, Ryan James Page
Graduate Theses and Dissertations
Reproductive performance is crucial for sustained financial success in the beef cattle industry. This dissertation includes a population study that quantified the incidence of Neospora caninum infections in the central region of the United States and tested its relationship with reproductive performance in beef cattle. Trial one of that study concluded that 6.9% of open, replacement heifers (n = 1306) tested seropositive. The second trial in that project found that 9.6% of the breeding age females (n = 500) tested were seropositive for Neospora caninum; and that state in which the cattle lived and age impacted (P < 0.05) infection rate. Breed composition, number of farm dogs on the ranch, and use of total mixed rations were not associated (P > 0.1) with seropositive …
Effect Of The Prospect Of Transport On Captive Tiger Behavior And Fecal Cortisol In Naïve And Experienced Tigers, Callan Lichtenwalter
Effect Of The Prospect Of Transport On Captive Tiger Behavior And Fecal Cortisol In Naïve And Experienced Tigers, Callan Lichtenwalter
Graduate Theses and Dissertations
With close to 5,000 captive tigers in the United States, it is vital to ensure that these tigers are receiving adequate welfare whether they are in an accredited facility, or privately owned. The goal of this study was to assess whether captive tigers at a rescue facility with experience being transported outside of the facility would respond differently to the presentation of their transport vehicle than their naïve counterparts who had only been transported within the facility. The behavior of 5 naïve and 7 experienced tigers (n = 12) located at Turpentine Creek Wildlife Refuge in Eureka Springs, Arkansas was …
Methods To Evaluate Ruminant Animal Production Responses, Jose Manuel Diaz Gomez
Methods To Evaluate Ruminant Animal Production Responses, Jose Manuel Diaz Gomez
Graduate Theses and Dissertations
In experiment 1, 80 steers (197.0 kg initial body weight; BW for fall, 116.9 kg for spring), were stocked at 2.45 and 4.1 calves/ha in fall and spring, respectively in 16 tall fescue pastures [fall ergovaline (EV) = 1,475 ppb and spring EV = 1,173 ppb] under 2 treatments, mineral (MIN) (n = 8) and cumulative management (CM) (n = 8). Forage allowance did not differ (P = 0.76) between CM and MIN during fall but differed during spring (P ≤ 0.05, 2.55 vs. 3.22 kg DM/kg BW, for MIN and CM, respectively). For fall, average daily gain (ADG) resulted …
Investigating Egg Storage Conditions, Hatch Characteristics, And Feeding Methods Of Commercially Produced Poultry, Joshua R. Deines
Investigating Egg Storage Conditions, Hatch Characteristics, And Feeding Methods Of Commercially Produced Poultry, Joshua R. Deines
Graduate Theses and Dissertations
Advancements in poultry research continue to add knowledge and understanding to an already technical industry. Such information has led to new developments in early feeding strategies which provide nutrients to broiler chicks while in the egg or during transport. This wealth of knowledge in poultry research extends beyond chickens and is utilized for other species, such as quail and pheasants, including how fertile eggs are stored and incubated. However, methods of early feeding broilers have not been investigated in industry relevant approaches to understand its effects, including how timing of feed access interacts with the hatch window. As well, quail …
Preparation And Evaluation Of Single-Stranded Dna Aptamer-Based Immunological Adjuvant In Broiler Chickens, Adil Sabr Al-Ogaili
Preparation And Evaluation Of Single-Stranded Dna Aptamer-Based Immunological Adjuvant In Broiler Chickens, Adil Sabr Al-Ogaili
Graduate Theses and Dissertations
Mineral oils and metal salts are commonly used as adjuvants to enhance acquired immunity. Recently, monoclonal antibodies (MAbs) and recombinant peptides agonist CD40 receptor have shown remarkable promise for induction of rapid and robust immune responses. Limitations of this approach MAb production costs and multiple administrations due to anti-MAb immune responses. Here we demonstrate the development of a unique and sophisticated DNA aptamer-based alternative for CD40-directed delivery of universal antigens as an alternative in chickens, and potentially other vertebrate species. This receptor, expressed by antigen-presenting cells, acts as a costimulatory molecule for activated T helper lymphocytes. After initially selecting for …
Assessment Of A Nutritional Rehabilitation Model In Two Modern Broilers And Their Jungle Fowl Ancestor: A Model For Better Understanding Childhood Undernutrition, Mikayla Baxter
Graduate Theses and Dissertations
The World Health Organization, estimated that 22.9% of children under the age of 5 are stunted. The etiology of stunting is multifactorial and is associated with poor linear growth, villous atrophy, dysbiosis, and increased intestinal permeability. Inclusion of rye in poultry diets induces nutrient deficiencies and increases intestinal permeability, dysbiosis and decreases growth rates. The objective of this dissertation was to determine if chickens consuming a rye based diet exhibited a similar pathophysiology of stunted children to develop a relevant animal model. Therefore, early or late phase malnutrition was induced determine the effects of malnutrition on performance, bone mineralization, intestinal …
Evaluation Of The Efficacy Of A Candidate Turkey Dermatitis/Cellulitis Oil Emulsion Vaccine On Immune Response, Morbidity, And Mortality Under Laboratory And Commercial Conditions, Brittany Danielle Graham
Evaluation Of The Efficacy Of A Candidate Turkey Dermatitis/Cellulitis Oil Emulsion Vaccine On Immune Response, Morbidity, And Mortality Under Laboratory And Commercial Conditions, Brittany Danielle Graham
Graduate Theses and Dissertations
Alpha-toxigenic Clostridium septicum (CS), the cause of turkey cellulitis, results in devastating mortality with high costs for the industry. Various vaccinations have been evaluated to prevent this disease with moderate success. Ability of a CS bacterin-toxoid, in conjunction with adjuvants such as aluminum hydroxide, mannoslyated chitosan, or a water-in-oil Seppic Montanide 71 R VG adjuvant (OE) to induce immunity was evaluated in a 7-week study (Experiment 1). Poults (20/group) were vaccinated day-of-hatch, boosted at 5 weeks-of-age and compared to unvaccinated controls. Antibody titers were determined by ELISA for all experiments. In experiment 1, initial vaccination with OE resulted in significantly …
Evaluation And Selection Of A Bacillus Based Direct-Fed Microbial Candidate For In Situ Enzyme Production To Improve Gut Health Integrity, Bone Quality And Growth Performance In Poultry, Juan David Latorre Cardenas
Evaluation And Selection Of A Bacillus Based Direct-Fed Microbial Candidate For In Situ Enzyme Production To Improve Gut Health Integrity, Bone Quality And Growth Performance In Poultry, Juan David Latorre Cardenas
Graduate Theses and Dissertations
During the last decade, the increasing interest in renewable energy sources has been changing the distribution of corn utilization from human and animal consumption to biofuel production, leading to a continuous rise in feed costs of livestock diets. Therefore, alternative feed ingredients such as distillers dried grains with solubles (DDGS), as well as cereals like wheat, barley, and sorghum have become part of the feed matrix to maintain or reduce production costs. However, these raw materials often contain a higher concentration of antinutritional factors in comparison to corn, including non-starch polysaccharides (NSP) which increase digesta viscosity and reduce nutrient absorption …
Efficacy Of A Novel Intranasal Zinc Solution On The Microbiome, Health, And Growth Performance Of High-Risk, Newly Received Stocker Cattle, Makenzie Foster
Efficacy Of A Novel Intranasal Zinc Solution On The Microbiome, Health, And Growth Performance Of High-Risk, Newly Received Stocker Cattle, Makenzie Foster
Graduate Theses and Dissertations
The objective of this study was to determine if using an intranasal zinc (Zn) solution would impact health and growth performance of high-risk stocker cattle. Male beef calves (n = 239; 3 arrival dates [block]; initial BW = 276 ± 2.4 kg) were stratified by arrival gender and BW and assigned to 1 of 2 treatments: 1) treated with 3 ml of a Zn solution containing 36.24 mg of Zn administered intranasally, or 2) control, in which calves were not treated. Calves were observed daily and if exhibiting signs of morbidity and a rectal temperature ≥ 40° C they were …
Efficacy Of Beta-Resorcylic Acid To Reduce Campylobacter Jejuni In Pre-Harvest And Post-Harvest Poultry, Basanta Raj Wagle
Efficacy Of Beta-Resorcylic Acid To Reduce Campylobacter Jejuni In Pre-Harvest And Post-Harvest Poultry, Basanta Raj Wagle
Graduate Theses and Dissertations
Campylobacteriosis is one of the leading foodborne illnesses in United States, and is associated with the consumption of poultry and poultry products. Reducing Campylobacter in these species will reduce the burden of this disease. Unfortunately, most strategies employed to reduce Campylobacter in poultry have either not been successful or produced inconsistent results. One potential control strategy is the use of β-resorcylic acid (BR), a phytophenolic compound classified by the US FDA as "Everything Added to Food in the United States" (EAF 3045) and is therefore deemed safe for consumption. This compounds has antibacterial activity against Salmonella, however, its efficacy to …
The Efficacy Of Probiotic Bacterial Isolates In Reducing Cecal Camylobacter Colonization In Broiler Chickens, Komala Arsi
The Efficacy Of Probiotic Bacterial Isolates In Reducing Cecal Camylobacter Colonization In Broiler Chickens, Komala Arsi
Graduate Theses and Dissertations
Campylobacter is the leading cause of foodborne illness worldwide and human illnesses are often associated with consumption of poultry or poultry products. Many strategies have been tried to eliminate Campylobacter from poultry with limited success. One of the strategies to reduce Campylobacter colonization in poultry is by use of probiotics. We conducted 2 separate studies to evaluate the efficacy of probiotics against Campylobacter in broiler chickens. For our first study, GRAS (Generally Regarded as Safe) bacteria were isolated from healthy chickens and tested their efficacy against Campylobacter in vitro. Twenty six isolates with in vitro anti-Campylobacter activity were …
Relationships Between Landscape Factors And Crayfish Density And The Interacting Effects Of Grazing And Increased Sedimentation On Algal Biomass, Katheryn Haley Rose
Relationships Between Landscape Factors And Crayfish Density And The Interacting Effects Of Grazing And Increased Sedimentation On Algal Biomass, Katheryn Haley Rose
Graduate Theses and Dissertations
In recent years in the United States there has been a rapid expansion in anthropogenic sources of sediment in streams including construction, agriculture, and drilling for natural gas. Potential effects land disturbance associated with activities from natural gas development on aquatic biota in surrounding streams have not yet been well documented. An increase in inorganic sediment in streams can be detrimental to organisms through a variety of mechanisms including alteration of dominant substrate type, higher turbidity resulting in lower visibility, and burial of food resources such as algae and detritus. Increasing sedimentation in stream environments through anthropogenic disturbance is a …
Identification Of Biomarkers Associated With Ascites Incidence In Broilers, Kaylee Rowland
Identification Of Biomarkers Associated With Ascites Incidence In Broilers, Kaylee Rowland
Graduate Theses and Dissertations
Poultry is key in genetic research due to breeding feasibility, relatively short generation interval, and distinct phenotypes. It is estimated that 8% of broiler deaths annually can be attributed to ascites, an economically important disease that has been challenging the industry for the past 2 decades. Genetically selected ascites resistant (RES) and susceptible (SUS) chicken lines have been established and maintained by the University of Arkansas, Fayetteville. Intensive research efforts have been made to reveal physiological and biochemical characteristics for the incidence of ascites. Since the whole genome of the major ancestral chicken, the Red Jungle Fowl, has been sequenced, …
Genetic Analysis Of The Promoter Region Of The Serotonin Receptor 5ht2b And Its Contribution To Pulmonary Hypertension Syndrome In Broiler-Type Chickens, Khaloud Awada Alzahrani
Genetic Analysis Of The Promoter Region Of The Serotonin Receptor 5ht2b And Its Contribution To Pulmonary Hypertension Syndrome In Broiler-Type Chickens, Khaloud Awada Alzahrani
Graduate Theses and Dissertations
Pulmonary Arterial Hypertension (PAH) is a sever disorder characterized by high pulmonary artery pressure and pulmonary vascular resistance along with specific histological changes. Idiopathic PAH is a human disease of unknown origin that showed common characteristics to PHS/ascites in chicken. PHS/ascites is a condition found in broilers causing right ventricular hypertrophy, valvular insufficiency, increased venous pressure, variable liver changes, accumulation of fluids in the abdominal cavity, and finally lung and heart failure. Currently, there is no acceptable animal model for human PAH. Our group has been validating the chicken as the medical animal model for human PAH, thus we have …
The Impact Of Bovine Spongiform Encephalopathy (Bse) On World Protein Supply, Jarrod Kersey
The Impact Of Bovine Spongiform Encephalopathy (Bse) On World Protein Supply, Jarrod Kersey
Graduate Theses and Dissertations
As world population, per capita income and urbanization increase, consumption of meat, poultry, milk and eggs will continue to rise to meet the needs of approximately nine billion people in 2050. Global beef, pig and chicken production and per capita consumption increased between 2000 and 2010, with growth in broiler production and consumption outpacing both beef and swine. The increased production and consumption requires readily available feed ingredients in regions where animal production is rapidly developing. Animal-based protein feed ingredients are often difficult to move from country to country due to real or perceived risk of animal disease. Zoosanitary standards …
Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam
Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam
Graduate Theses and Dissertations
The present study investigated the distribution of the vasotocin subtype four receptor (VT4R) in brain and pituitary gland of the chicken, Gallus gallus. The anterior pituitary cell types associated with the VT4R were also determined. Two polyclonal antibodies were raised in rabbit against a cocktail of peptides, 15 amino acids from the amino terminal region and 17 amino acids from the carboxy terminal region of VT4R receptor. The antibody was validated utilizing the Western blot and immunocytochemistry. A single band at 47KDa utilizing membrane protein extracts of chicken brain and pituitary tissues was shown for the VT4R antibody. A peptide …