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Analytical, Diagnostic and Therapeutic Techniques and Equipment
Atomic force microscopy | Antigens | Ferritin | Carrier proteins
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Development Of An Atomic Force Microscopic Immunosensor For The Ferritin Antigen, Brendan O'Connell
Development Of An Atomic Force Microscopic Immunosensor For The Ferritin Antigen, Brendan O'Connell
Theses
The adsorption conditions for a range of different immunoreactants to both polystyrene and ultrasmooth silanised silicon surfaces are optimised. The conditions studied were concentration of protein, time and temperature of incubation, pH, molarity and ionic strength of the buffer. The anti-ferritin surface was selected as our model system. This immunosensor was used to develop and evaluate a millispot ELISA for ferritin. The millispot ferritin ELISA developed utilises a sample volume of 6pl and a sample incubation time of 10 minutes. Precision and accuracy studies show the assay to have a dynamic assay range of 7.8-lOOng/ml ferritin. The millispot ELISA detected …