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RNA-seq

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Establishing A Permanent Feeding Site: Reniform Nematode Manipulation Of Soybean Genes, Nathan Wayne Redding May 2015

Establishing A Permanent Feeding Site: Reniform Nematode Manipulation Of Soybean Genes, Nathan Wayne Redding

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Reniform nematode, Rotelynchulus reniformis, is a semi-endoparasite capable of infecting more that 300 host plant species in tropical, subtropical, and warm temperate regions. Female nematodes penetrate host roots and introduce effectors that lead to the formation of multinucleate feeding sites called syncytia. The objective of our study was to identify plant genes involved in the process of syncitium formation. We used a split-root system in which half of a plant’s roots were inoculated with R. reniformis while the rest of the root system remained uninfected. Illumina RNA-seq was used to quantify gene expression patterns in replicate samples of infected and …