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Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Mapping The Transcriptional Transactivation Function Of Simian Virus 40 Large T Antigen., Jiyue Y. Zhu, Philip W. Rice, Michele Chamberlain, Charles N. Cole
Mapping The Transcriptional Transactivation Function Of Simian Virus 40 Large T Antigen., Jiyue Y. Zhu, Philip W. Rice, Michele Chamberlain, Charles N. Cole
Dartmouth Scholarship
T antigen is able to transactivate gene expression from the simian virus 40 (SV40) late promoter and from several other viral and cellular promoters. Neither the mechanisms of transactivation by T antigen nor the regions of T antigen required for this activity have been determined. To address the latter point, we have measured the ability of a set of SV40 large T antigen mutants to stimulate gene expression in CV-1 monkey kidney cells from the SV40 late promoter and Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter. Transactivation, although reduced, was retained by an N-terminal 138-amino-acid fragment of T …
Auxin-Regulated Gene Expression, Tom J. Guilfoyle
Auxin-Regulated Gene Expression, Tom J. Guilfoyle
Journal of the Iowa Academy of Science: JIAS
A number of cDNA clones to independent mRNAs that increase in amount after brief exposure to auxins have been isolated. From soybean, sequenced, and characterized. Increases in mRNA abundance can be detected as early as 2.5 minutes after auxin application to excised elongating regions of soybean hypocotyls. Increased transcription rates on the genes which encode the auxin-responsive mRNAs account, at least partly, for the accumulation of mRNAs. A variety of auxins induce the accumulation of the mRNAs, but other plant hormones have no effect on the mRNA abundance. Some of the genes which encode the mRNAs are clustered in the …
Temporal Analysis And Spatial Mapping Of Lymantria Dispar Nuclear Polyhedrosis Virus Transcripts And In Vitro Translation Polypeptides, James M. Slavicek
Temporal Analysis And Spatial Mapping Of Lymantria Dispar Nuclear Polyhedrosis Virus Transcripts And In Vitro Translation Polypeptides, James M. Slavicek
USDA Forest Service / UNL Faculty Publications
Genomic expression of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) was studied. Viral specific transcripts expressed in cell culture at various times from 2 through 72 h postinfection were identified and their genomic origins mapped through Northern analysis. Sixty-five distinct transcripts were identified in this analysis. Most viral transcripts were expressed late in infection, and originated from throughout the viral genome. Viral polypeptides expressed in infected 652Y cells were labeled with [35S]methionine and identified by autoradiography after separation by SDS polyacrylamide gel electrophoresis. Viral protein synthesis was found to occur in a sequential manner. Four proteins were …
Regulation Of Phytochrome Gene Expression, J. T. Colbert, S. A. Costigan, P. Avissar, Z. Zhao
Regulation Of Phytochrome Gene Expression, J. T. Colbert, S. A. Costigan, P. Avissar, Z. Zhao
Journal of the Iowa Academy of Science: JIAS
In etiolated oat seedlings exposure to red light results in a decrease in the transcription of the phytochrome genes, the abundance of phytochrome mRNA, and the level of phytochrome protein. Phytochrome itself serves as the photoreceptor for the response of decreased mRNA and transcription levels. The decrease in phytochrome mRNA is sensitive to low levels of Pfr. Even green "safelight" is capable of inducing a decrease in phytochrome mRNA abundance. Barley phytochrome mRNA abundance is also dramatically down-regulated in response to red light but other plant species vary in their ability to decrease phytochrome mRNA abundance after red light treatment. …