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A Technique For Physiologically Age-Grading Female Stable Flies, Stomoxys Calcitrans (L.), P. J. Scholl

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

A method of dissection followed by staining was initiated to allow physiological age- grading based on ovarian development in order to more completely describe the physiological development of adult female stable flies [Stomoxys calcitrans (L. )]. The gonotrophic developmental continuum from a non-differentiated cell in teneral females to mature eggs at the time of oviposition was first arbitrarily divided into six stages by using distinct landmarks within the developing oocyte . Then, nulliparous, uniparous, and biparous+ females were differentiated on the basis of the presence or absence of stained follicular relics in the ovariole sheaths. The combination of the …