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Isolation, Characterization, And Cross‐Amplification Of 20 Microsatellite Markers For Conospermum Undulatum (Proteaceae), Nicola Delnevo, Andrea Piotti, Eddie J. Van Etten, William D. Stock, Margaret Byrne
Isolation, Characterization, And Cross‐Amplification Of 20 Microsatellite Markers For Conospermum Undulatum (Proteaceae), Nicola Delnevo, Andrea Piotti, Eddie J. Van Etten, William D. Stock, Margaret Byrne
Research outputs 2014 to 2021
PREMISE: Recent habitat fragmentation is posing a risk to the wavy‐leaved smokebush, Conospermum undulatum (Proteaceae), a rare plant species endemic to southwestern Western Australia. Microsatellite markers are required to characterize the genetic diversity and structure of the species for conservation purposes and to facilitate ecological studies.
METHODS AND RESULTS: Illumina MiSeq high‐throughput sequencing was used to develop 20 novel microsatellite markers for C. undulatum. Polymorphism at each locus was assessed using 72 individuals from three natural populations. Nineteen markers were polymorphic, with the number of alleles per locus ranging from two to 21, and observed and expected heterozygosity ranging from …