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Full-Text Articles in Life Sciences
Controls Of Nucleosome Positioning In The Human Genome, Daniel J. Gaffney, Graham Mcvicker, Athma A. Pai, Yvonne N. Fondufe-Mittendorf, Noah Lewellen, Katelyn Michelini, Jonathan Widom, Yoav Gilad, Jonathan K. Pritchard
Controls Of Nucleosome Positioning In The Human Genome, Daniel J. Gaffney, Graham Mcvicker, Athma A. Pai, Yvonne N. Fondufe-Mittendorf, Noah Lewellen, Katelyn Michelini, Jonathan Widom, Yoav Gilad, Jonathan K. Pritchard
Molecular and Cellular Biochemistry Faculty Publications
Nucleosomes are important for gene regulation because their arrangement on the genome can control which proteins bind to DNA. Currently, few human nucleosomes are thought to be consistently positioned across cells; however, this has been difficult to assess due to the limited resolution of existing data. We performed paired-end sequencing of micrococcal nuclease-digested chromatin (MNase-seq) from seven lymphoblastoid cell lines and mapped over 3.6 billion MNase-seq fragments to the human genome to create the highest-resolution map of nucleosome occupancy to date in a human cell type. In contrast to previous results, we find that most nucleosomes have more consistent positioning …
Condensin Ii Promotes The Formation Of Chromosome Territories By Inducing Axial Compaction Of Polyploid Interphase Chromosomes, Christopher R. R. Bauer, Tom A. Hartl, Giovanni Bosco
Condensin Ii Promotes The Formation Of Chromosome Territories By Inducing Axial Compaction Of Polyploid Interphase Chromosomes, Christopher R. R. Bauer, Tom A. Hartl, Giovanni Bosco
Dartmouth Scholarship
The eukaryotic nucleus is both spatially and functionally partitioned. This organization contributes to the maintenance, expression, and transmission of genetic information. Though our ability to probe the physical structure of the genome within the nucleus has improved substantially in recent years, relatively little is known about the factors that regulate its organization or the mechanisms through which specific organizational states are achieved. Here, we show that Drosophila melanogaster Condensin II induces axial compaction of interphase chromosomes, globally disrupts interchromosomal interactions, and promotes the dispersal of peri-centric heterochromatin. These Condensin II activities compartmentalize the nucleus into discrete chromosome territories and indicate …
Mecp2 Binds To Nucleosome Free (Linker Dna) Regions And To H3k9/H3k27 Methylated Nucleosomes In The Brain, Anita A. Thambirajah, Marlee K. Ng, Lindsay J. Frehlick, Andra Li, Jason J. Serpa, Evgeniy V. Petrotchenko, Begonia Silva-Moreno, Kristal K. Missiaen, Christoph H. Borchers, J. Adam Hall, Ryan Mackie, Frank Lutz, Brent E. Gowen, Michael Hendzel, Philippe T. Georgel, Juan Ausió
Mecp2 Binds To Nucleosome Free (Linker Dna) Regions And To H3k9/H3k27 Methylated Nucleosomes In The Brain, Anita A. Thambirajah, Marlee K. Ng, Lindsay J. Frehlick, Andra Li, Jason J. Serpa, Evgeniy V. Petrotchenko, Begonia Silva-Moreno, Kristal K. Missiaen, Christoph H. Borchers, J. Adam Hall, Ryan Mackie, Frank Lutz, Brent E. Gowen, Michael Hendzel, Philippe T. Georgel, Juan Ausió
Biological Sciences Faculty Research
Methyl-CpG-binding protein 2 (MeCP2) is a chromatin-binding protein that mediates transcriptional regulation, and is highly abundant in brain. The nature of its binding to reconstituted templates has been well characterized in vitro. However, its interactions with native chromatin are less understood. Here we show that MeCP2 displays a distinct distribution within fractionated chromatin from various tissues and cell types. Artificially induced global changes in DNA methylation by 3-aminobenzamide or 5-aza-2′-deoxycytidine, do not significantly affect the distribution or amount of MeCP2 in HeLa S3 or 3T3 cells. Most MeCP2 in brain is chromatin-bound and localized within highly nuclease-accessible regions. We …
Examination Of The Chromatin Structure Of Xlr3b Using The Chromosome Conformation Capture Assay, Sarah Elise Conderino
Examination Of The Chromatin Structure Of Xlr3b Using The Chromosome Conformation Capture Assay, Sarah Elise Conderino
Honors Scholar Theses
Imprinted genes contain epigenetic modifications that influence expression patterns based on parent-of-origin. Recent studies have shown that imprinted genes contribute to numerous human diseases and disorders. Xlr3b, an imprinted gene on the X chromosome, has been implicated in social and behavioral deficits characteristic of disorders such as Turner syndrome and autism. The imprinting mechanism of this gene is still unknown, and this study analyzed the native chromatin structure of Xlr3b through the chromosome conformation capture assay to determine if there are any long-range interactions that regulate the expression of this gene. Brain tissue from a mouse model of Turner …