Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

Antimicrobial

Selected Works

Articles 1 - 5 of 5

Full-Text Articles in Life Sciences

The New Penicillin?, Nikki Huynh Apr 2017

The New Penicillin?, Nikki Huynh

Nikki Huynh

Clostridium difficile infects and kills 30,000-40,000 people a year in the United States. In order to target and minimize the spread of C. difficile we are screening puupehenone, a natural marine product that is excreted from some species of sponges and coral as a defense mechanism, and repurposing its role for medical use. Our research primarily focuses on determining puupehenone’s antimicrobial properties. This was determined by testing the minimum inhibitory concentration (MIC) of puupehenone as it relates to a single strain of C. difficile, and then observing whether the antimicrobial properties hold across multiple strains of the bacterium. The strain …

Go to article

Phytochemical Analysis And Biological Activities Of Cola Nitida Bark, Durand Dah-Nouvlessounon, Mamoudou H. Dicko Prof., Lamine Baba-Moussa Jan 2015

Phytochemical Analysis And Biological Activities Of Cola Nitida Bark, Durand Dah-Nouvlessounon, Mamoudou H. Dicko Prof., Lamine Baba-Moussa

Pr. Mamoudou H. DICKO, PhD

The aim of this study is to investigate some biological effects of Cola nitida’s bark after phytochemical screening. The bark was collected, dried, and then powdered for the phytochemical screening and extractions. Ethanol and ethyl acetate extracts of C. nitida were used in this study. The antibacterial activity was tested on ten reference strains and 28 meat isolated Staphylococcus strains by disc diffusionmethod. The antifungal activity of three fungal strains was determined on the Potato-Dextrose Agar medium mixed with the appropriate extract. The antioxidant activity was determined by DPPH and ABTS methods. Our data revealed the presence of various potent …

Go to article

Recombinant Expression Of Indolicidin Concatemers In Escherichia Coli, K Morin, S Arcidiacono, Richard Beckwitt, C Mello Feb 2013

Recombinant Expression Of Indolicidin Concatemers In Escherichia Coli, K Morin, S Arcidiacono, Richard Beckwitt, C Mello

Richard D Beckwitt

Antimicrobial peptides are part of the innate immune system of vertebrates and invertebrates. They are active against gram-negative and gram-positive bacteria, fungi, and protozoa. Currently, most antimicrobial peptides are extracted from host organisms or produced by solidphase peptide synthesis. Recombinant protein expression in Escherichia coli is a tool for greater production yields at a decreased cost and reduces the use of hazardous materials. We have constructed a concatamer of indolicidin and successfully expressed a fusion product with thioredoxin in E. coli BL21DE3. Codons for methionine residues flanking individual indolicidin genes were incorporated for cyanogen bromide cleavage of the fusion protein …

Go to article

Conjugating Berberine To A Multidrug Resistance Pump Inhibitor Creates An Effective Antimicrobial, John Bremner, Siritron Samosorn, Anthony Ball, Gabriele Casadei, Terence Moy, Frederik Ausubel, Kim Lewis Jan 2013

Conjugating Berberine To A Multidrug Resistance Pump Inhibitor Creates An Effective Antimicrobial, John Bremner, Siritron Samosorn, Anthony Ball, Gabriele Casadei, Terence Moy, Frederik Ausubel, Kim Lewis

Kim Lewis

No abstract provided.

Go to article

Chemical Composition And Antimicrobial Activity Of Cymbopogon Citratus And Cymbopogon Giganteus Essential Oils Alone And In Combination, Aline Lamien-Meda, Balé Bayala, Clément L. Obamé, André J. Ilboudo, Christian Franz, J Novak, Roger C. Nebié, Mamoudou H. Dicko Prof. Sep 2011

Chemical Composition And Antimicrobial Activity Of Cymbopogon Citratus And Cymbopogon Giganteus Essential Oils Alone And In Combination, Aline Lamien-Meda, Balé Bayala, Clément L. Obamé, André J. Ilboudo, Christian Franz, J Novak, Roger C. Nebié, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

As part of ongoing research on the chemical composition and the antimicrobial properties of Burkinabe plants essential oils alone and in combination, essential oils (EOs) from leaves of Cymbopogon citratus and Cymbopogon giganteus from Burkina Faso were analyzed by GC–FID and GC–MS. Five constituents, which accounted for 96.3% of the oil, were identified in the EO of C. citratus. Geranial (48.1%), neral (34.6%) and myrcene (11.0%) were the major constituents. For C. giganteus a total of eight compounds were identified which represented 86.0% of the oils extracted. The dominant compounds were limonene (42%) and a set of monoterpene alcohols: trans-p-mentha-1(7),8-dien-2-ol …

Go to article